Immunochromatographic enhancement strategy for SARS-CoV-2 detection based on nanotechnology

Sun, Qingwen; Ning, Qihong; Li, Tangan; Jiang, Qixia; Feng, Shaoqing; Tang, Ning; Cui, Daxiang; Wang, Kan

doi:10.1039/d3nr02396f

Cited by 2 publications

(1 citation statement)

References 127 publications

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“…It has been reported that some commercially available rapid antigen detection kits are 10 2 -10 5 times less sensitive than RT-PCR [27][28][29]. Great efforts have been made to enhance the sensitivity of LFIA for detecting SARS-CoV-2 [30]. For example, Ruantip et al incorporated an enhancement pad to a conventional LFIA and achieved a 10-fold improvement in sensitivity (i.e., 0.5 ng/mL N protein) [31].…”

Section: Discussionmentioning

confidence: 99%

Development of Detection Antibody Targeting the Linear Epitope in SARS-CoV-2 Nucleocapsid Protein with Ultra-High Sensitivity

Wu,

Jiang,

Yang

et al. 2024

IJMS

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The COVID-19 pandemic caused by SARS-CoV-2 highlighted the importance of reliable detection methods for disease control and surveillance. Optimizing detection antibodies by rational screening antigens would improve the sensitivity and specificity of antibody-based detection methods such as colloidal gold immunochromatography. In this study, we screened three peptide antigens with conserved sequences in the N protein of SARS-CoV-2 using bioinformatical and structural biological analyses. Antibodies that specifically recognize these peptides were prepared. The epitope of the peptide that had the highest binding affinity with its antibody was located on the surface of the N protein, which was favorable for antibody binding. Using the optimal antibody that can recognize this epitope, we developed colloidal gold immunochromatography, which can detect the N protein at 10 pg/mL. Importantly, this antibody could effectively recognize both the natural peptide antigen and mutated peptide antigen in the N protein, showing the feasibility of being applied in the large-scale population testing of SARS-CoV-2. Our study provides a platform with reference significance for the rational screening of detection antibodies with high sensitivity, specificity, and reliability for SARS-CoV-2 and other pathogens.

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Section: Discussionmentioning

confidence: 99%

Development of Detection Antibody Targeting the Linear Epitope in SARS-CoV-2 Nucleocapsid Protein with Ultra-High Sensitivity

Wu,

Jiang,

Yang

et al. 2024

IJMS

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show abstract

Advances in design and preparation of nanozymes and their applications for constructing higher sensitive lateral flow assays

Li,

Peng,

Huang

et al. 2024

Coordination Chemistry Reviews

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Immunochromatographic enhancement strategy for SARS-CoV-2 detection based on nanotechnology

Abstract: This review summarizes new methods for improving the sensitivity of immunochromatographic SARS-CoV-2 detection based on nanotechnology.

Cited by 2 publications

References 127 publications

Development of Detection Antibody Targeting the Linear Epitope in SARS-CoV-2 Nucleocapsid Protein with Ultra-High Sensitivity

Development of Detection Antibody Targeting the Linear Epitope in SARS-CoV-2 Nucleocapsid Protein with Ultra-High Sensitivity

Advances in design and preparation of nanozymes and their applications for constructing higher sensitive lateral flow assays

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