Immunochromatographic assay for T-2 toxin based on luminescent quantum dot beads

Qie, Zhiwei; Shi, Jinmiao; Yan, Wenliang; Gao, Zichen; Wu, Meng; Xiao, Rui; Wang, Shengqi

doi:10.1039/c9ra06689f

Cited by 7 publications

(1 citation statement)

References 27 publications

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“…Lateral flow immunoassay performance was evaluated in the sense of pre-treatment given to the sample pad. Many reports showed that when the sample pad was pre-treated with a dilution buffer with surfactants, such as Tween 20 or PEG, it increased the sample flow, eliminated the background, and increased the sensitivity and stability of the LFIA [ 35 ]. In our study, we evaluated the sample running buffer by testing the buffer with the different concentrations of Tween-20 ranging from 0.01 to 0.15% and finally, a pre-treatment solution consisting of 0.1% Tween-20 was selected ( Supplementary Figure S2 ).…”

Section: Resultsmentioning

confidence: 99%

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker

Natarajan

DeRosa

Shah

et al. 2021

Sensors

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The diagnosis, prognosis, and control of chronic kidney disease rely on an understanding of the glomerular filtration rate (GFR). The renal clearance of the cystatin-C is closely associated with the GFR. Cystatin-C is a more suitable GFR marker than the commonly used creatinine. General techniques for cystatin-C calculation, such as particle-enhanced turbidimetric and nephelometric assay, are time-consuming and tedious. Here, we propose a rapid, quantitative immunoassay for the detection of cystatin-C. A fluorescence-based lateral-flow kit was developed in a sandwich format by using a monoclonal antibody. A Linear calibration was obtained over the clinical diagnostic range of 0.023–32 µg/mL and the limit of detection (LOD) was 0.023 µg/mL and the limit of quantification (LOQ) was 0.029 µg/mL. Average recoveries from spiked urine samples ranged from 96–100% and the coefficient of variation was less than 4% for both intra and inter-day assays with excellent repeatability. With the comparison with an ELISA kit, the developed kit is highly sensitive, performs well over the detection range, provides repeatable results in a short time, and can easily be used at point-of-care (POC), making it an ideal candidate for rapid testing in early detection, community screening for renal function disorders.

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Section: Resultsmentioning

confidence: 99%

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker

Natarajan

DeRosa

Shah

et al. 2021

Sensors

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show abstract

Development of two immunochromatographic test strips based on signal amplification and selenium nanoparticles for the rapid detection of T-2 mycotoxin

et al. 2023

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An electrochemiluminescence biosensor based on target-responsive DNA hydrogel for T-2 toxin

Cai,

Li,

Luo

et al. 2024

Microchemical Journal

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Immunochromatographic assay for T-2 toxin based on luminescent quantum dot beads

Abstract: A quantum dot bead based immunochromatographic assay was established for T-2 toxin with a limit of detection of 0.08 ng mL−1.

Cited by 7 publications

References 27 publications

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker

Development of two immunochromatographic test strips based on signal amplification and selenium nanoparticles for the rapid detection of T-2 mycotoxin

An electrochemiluminescence biosensor based on target-responsive DNA hydrogel for T-2 toxin

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