Immunochromatographic analysis of bovine growth hormone releasing factor involving reversed-phase high-performance liquid chromatography-immunodetection

Cho, Byung-Yun; Zou, Hanfa; Strong, Richard A.; Fisher, Daniel H.; Nappier, John; Krull, Ira S.

doi:10.1016/0021-9673(96)00356-1

Cited by 21 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This assay has been used with acridinium ester labels for the determination of parathyroid hormone in plasma [162,163]. A sandwich immunoassay has also been used for the postcolumn detection of bovine growth-hormone releasing factor [164]. A sandwich immunoassay has better selectivity than most competitive-binding immunoassay methods, due to its use of two antibodies for each analyte; a sandwich immunoassay also gives a signal that is directly proportional to the analyte's concentration.…”

Section: Use Of Silica and Affinity Ligands For Indirect Detectionmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

show abstract

Section: Use Of Silica and Affinity Ligands For Indirect Detectionmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Irth et al presented different strategies to accomplish immunochemical interactions in on-line post-column detection techniques (Irth et al, 1995). Either labeled antibodies (Irth et al, 1993;Oosterkamp et al, 1994aOosterkamp et al, 1998Irth et al, 1995;Miller and Herman, 1996;Cho et al, 1996) or labeled antigens (Oosterkamp et al, 1994b;Irth et al, 1995;Vanderlaan et al, 1995;Oosterkamp et al, 1996aOosterkamp et al, , 1996bOosterkamp et al, , 1997aOosterkamp et al, , 1997bCho et al, 1996) can be used to detect analytes eluting from the HPLC column. Bound and unbound labeled antibodies or labeled antigens are separated prior to detection.…”

Section: Post-column Immunoassay Coupled To Lcmentioning

confidence: 99%

“…Other formats of immunoassay used in the postcolumn mode include direct detection immunoassays (Vanderlaan et al, 1995;Cho et al, 1996), one-site immunoassays (Irth et al, 1993(Irth et al, , 1995Oosterkamp et al, 1994aOosterkamp et al, , 1997aOosterkamp et al, , 1998Miller and Herman, 1996), sandwich immunoassays (Cho et al, 1996) and competitive binding immunoassays (Oosterkamp et al, 1994b(Oosterkamp et al, , 1996a(Oosterkamp et al, , 1996b(Oosterkamp et al, , 1997a(Oosterkamp et al, , 1997bIrth et al, 1995;Shahdeo and Karnes, 1999;Shahdeo et al, 2000;Graefe et al, 2000).…”

Section: Post-column Immunoassay Coupled To Lcmentioning

confidence: 99%

Coupling immunoassays with chromatographic separation techniques

Tang

Karnes

2000

Biomed. Chromatogr.

View full text Add to dashboard Cite

Coupling immunoassays with HPLC separation techniques is becoming increasingly useful in the analysis of biological and nonbiological samples of both large and small molecules. This is because it provides both sensitivity and selectivity for molecular analysis at relatively low cost, low maintenance and with excellent potential for automation. This paper reviews application of this hyphenated approach both in the pre-column immunoextraction and post-column immunodetection modes. Systems in which immunoassays are interfaced to chromatographic separations in order to separate bound and free fractions of the immunoassay will not be included since these systems do not provide the enhanced selectivity common to hyphenated systems. Post-column immunodetection is based on various immunoassay formats such as direct detection, one-site, competitive and sandwich immunoassays. Homogeneous immunodetectors are more convenient than heterogeneous immunodectors since there are no separation and column regeneration steps involved in homogeneous immunoassays. On the other hand, heterogeneous immunoassays are generally more sensitive than homogeneous immunoassays since interfering substances are removed prior to immunodetection. Advantages and limitations for the various approaches will be discussed.

show abstract

“…By the proper choice of labels and detection system, the sensitivity of the system can also be enhanced. There are several examples of post-column immunodetection following HPLC (Irth et al, 1993;Oosterkamp et al, 1994Oosterkamp et al, , 1996aCho et al, 1996;Miller and Herman, 1996;Lutz et al, 1996). Some of these homogeneous assay formats used and others were performed in the heterogeneous mode.…”

Section: Introductionmentioning

confidence: 99%

Post-column reaction detection of biotin in human plasma ultrafiltrate based on laser-induced fluorescence energy transfer in the far-red spectral region

Shahdeo

Anderson

Karnes

2000

Biomed. Chromatogr.

View full text Add to dashboard Cite

High performance liquid chromatography followed by post-column reaction detection in the far-red spectral region provides added sensitivity and selectivity. A homogeneous fluorescence energy transfer assay in the competitive mode based on the binding of biotin and streptavidin was developed as an on-line post-column reaction detection system. The labels used for energy transfer were R-Phycoerythrin conjugated to biotin and Cyanine 5 labeled with streptavidin. The energy transfer peak was measured at 670 nm and excitation was achieved using the 488 nm line of an argon ion laser. The biotin concentration in plasma ultrafiltrate ranged from 0.024 to 6.12 ng/mL (n = 6). The precision of the two controls, 0.24 and 2. 44 ng/mL, was found to be 18.70% and 9.92% relative standard deviation respectively. Accuracy was 10.47% and 1.95% difference from spiked, respectively (n = 6). The limit of detection was 21.70 pg/mL (8.90 x 10(-11)M) calculated based on a factor of 2x the standard deviation of the blank (n = 6). The correlation coefficient for the calibration curve was found to be 0.9995. Recovery from plasma ultrafiltrate at 2.44 ng/mL was 103.40% (n = 6). Detection selectivity was indicated by the absence of background fluorescence in six different plasma samples collected from six individual donors. Endogenous levels were detected in two of the six pools of plasma ultrafiltrates.

show abstract

Immunochromatographic analysis of bovine growth hormone releasing factor involving reversed-phase high-performance liquid chromatography-immunodetection

Cited by 21 publications

References 20 publications

Applications of silica supports in affinity chromatography

Applications of silica supports in affinity chromatography

Coupling immunoassays with chromatographic separation techniques

Post-column reaction detection of biotin in human plasma ultrafiltrate based on laser-induced fluorescence energy transfer in the far-red spectral region

Contact Info

Product

Resources

About