Immunochemically-active cross-linked polystyrene preparations

Rapid Assays for the Detection and Determination of Sparse Populations of Bacteria and Bacteriophage T7 with Radioactively Labelled Homologous Antibodies

“…The yield of soluble protein was about 3 mg. Quantitative precipitin tests (Kabat & Mayer, 1961) showed that the immunological activity of the purified antibody was only eight-to tenfold that of the parent antiserum. This was a relatively low purification factor, but no higher specific immunological activity was obtained with immunoadsorbants of rabbit globulins polymerized with polystyrene (Kent & Slade, 1960) or 'Immunothiol' (Stephen, Gallop & Smith, 1966).…”

Production and characterization of a genetically engineered anti-caffeine camelid antibody and its use in immunoaffinity chromatography

“…To further investigate the antibody's tolerance to extremes of pH and ionic strength, additional stability screens were performed over a wide range of pH (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and salt concentrations (0-1000 mM). Fig.…”

“…Applications range from diagnostics, e.g., immunoassays, arrays, and sensors, to therapeutics, e.g., biodrugs and cancer-specific drug delivery agents [1][2][3][4][5][6]. As early as five decades ago, solid-phase immobilized antibodies were first utilized for the adsorption and separation, respectively, of protein antigens [7][8][9][10]. Although, especially in the early days of immunoaffinity chromatography, polyclonal antibodies have successfully been used for the separation and purification of both high-and low-molecular weight targets, monoclonal antibodies quickly became the receptor molecules of choice after their introduction by Köhler and Milstein in 1975 [11].…”

Separation of antigens by immunological specificity. Use of cellulose-linked antibodies as immunosorbents

“…McDuffie & Sutherland (1963) coagulated antigenantibody complexes by heating and by chemical treatment, and claimed that these preparations were capable of specific adsorption and subsequent desorption of the homologous antigen. Antibodies have also been linked covalently to diazotized amino-polystyrene (Kent & Slade, 1960) and to cellulose (Jagendorf, Patchornick & Sela, 1963), but with both these preparations the adsorption of antigen was not specific. Recently DeCarvalho, Lewis, Rand & Uhrick (1964) have developed immunosorbents in which bisdiazotized benzidine was used as a cross-linking agent for immune y-globulins, and Olovnikov (1964) has made similar preparations with 4,4'-diaminodiphenylamine.…”