Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide

Shibata, Nobuyuki; Ichikawa, Tsutomu; Tojo, Minehiro; Takahashi, Michio; Ito, Norio; Okubo, Y; Suzuki, Shigeo

doi:10.1016/0003-9861(85)90511-9

Cited by 117 publications

(78 citation statements)

References 33 publications

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“…Remarkably, both F2, were essentially mannoproteins, similar in chemical immunoenhancing and immunosuppressive effects have composition to those obtained by others (Domer et a / . , been ascribed to this material (Carrow & Domer, 1985;Shibata et a/., 1985Shibata et a/., , 1986 (Podzorski et al, 1990). Despite the similarities in chemical composition, there must be important, immunologically relevant, molecular differences between the two mannoprotein fractions separated here, as only F2 had the whole spectrum of immunological activities shown by the crude extract.…”

Section: Discussionmentioning

confidence: 97%

Lymphoproliferative and cytotoxic responses of human peripheral blood mononuclear cells to mannoprotein constituents of Candida albicans

Torosantucci

Palma²,

Boccanera³

et al. 1990

Journal of General Microbiology

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Two major proteoglycan constituents (designated F1 and F2) of the cell wall of Candida albicans were separated by ion-exchange chromatography from a crude carbohydrate-rich extract (GMP), and investigated for their chemical and molecular composition, antigenicity and immunomodulatory properties in cultures of human peripheral blood mononuclear cells (PBMC). Both fractions consisted predominantly of Periodic acidSchitf (PAS) and concanavalin A (Con A)-reactive material consisting of > 90 % mannose, 3-5% protein and small amounts of phosphorus; each was recognized by an anti-Candida rabbit serum as well as by a monoclonal antibody (mAb AF1) directed against an oligosaccharide epitope present on the fungal cell surface. When F1 and F2 were subjected to SDS-PAGE, transblotted and stained with enzyme-conjugated mAb AF1 or Con A, most of the antibody or lectin bound to high molecular mass (> 200 kDa) polydisperse material, some of which was present in F2 (as in the starting GMP extract) but absent in F1. This difference was also observed in PAS-stained gels of the two fractions.The F2, but not the F1, constituent was as active as the unfractionated GMP extract in inducing lymphoproliferation, production of the cytokines interleukin-2 and interferon-y, and generation of cytotoxicity against a natural-killer-sensitive target cell line (K562). These immunomodulatory properties were, like those possessed by GMP, protease-sensitive and heat-stable. Treatment of PBMC cultures with a modulatory anti-Tcell receptor antibody abolished the lymphoproliferation induced by GMP and F2 but not that induced by phytohaemagglutinin, showing that the mannoprotein materials of C. albicans acted through interaction with the antigen receptor complex.

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Section: Discussionmentioning

confidence: 97%

Lymphoproliferative and cytotoxic responses of human peripheral blood mononuclear cells to mannoprotein constituents of Candida albicans

Torosantucci

Palma²,

Boccanera³

et al. 1990

Journal of General Microbiology

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“…The C albieans J-1012 (serotype A), NIH B-792 (serotype B), and Candida stellatoidea IFO 1397 (synonym of C albicans serotype B) strain cells were the same specimens used in the previous studies [6,17]. GDP-mannose was obtained from Sigma Products.…”

Section: Methodsmentioning

confidence: 99%

“…Candida albicans strains were divided into two serotypes, A and ~, by Hasenclever and Mitchell [3] from the antigenicity of cell vall mannans. A structural study of the mannans of the 2 albicans serotype A and B strains has been achieved during he last several years [4,5], and we have demonstrated the pres-'nce of phosphodiesterified fl-l,2-1inked oligomannosyl moieies as a group of common epitopes throughout the two seroype strains [6,7]. The major difference between the structural !eatures of mannans from C. albicans serotype A and B strain .…”

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confidence: 89%

Detection of β‐1,2‐mannosyltransferase in Candida albicans cells

et al. 1995

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A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% ~1 riton X-100. Incubation of this fraction with a mannopentaose, Man~l-->2Manotl-->(2Manal-Qz2Man (al~Mans), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by ~H NMR indicates that a~Man5 was changed to Man~l--~ 2Manl~ll--~2Manal-~(2Manal--~)z 2Man (a~lMan6). This gl-l,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn 2÷ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albieans NIH B-792 (serotype B) strain calls, the mannan of which does not possess both the otl3Mans and airMan6 side chains, also exhibited the same/3-1,2-ManTase II activity. /(ey words: Candida albicans; fl-1,2-Linked mannose; Mannosyltransferase ~. IntroductionCandidiasis is an infectious disease frequently seen in early hildhood and in adults with predisposing conditions such as ,tiabetes, cancer, AIDS, and immuno-suppressive therapy [1,2]. Candida albicans strains were divided into two serotypes, A and ~, by Hasenclever and Mitchell [3] from the antigenicity of cell vall mannans. A structural study of the mannans of the 2 albicans serotype A and B strains has been achieved during he last several years [4,5], and we have demonstrated the pres-'nce of phosphodiesterified fl-l,2-1inked oligomannosyl moieies as a group of common epitopes throughout the two seroype strains [6,7]. The major difference between the structural !eatures of mannans from C. albicans serotype A and B strain .',ells is the presence of a fl-1,2-1inked mannose unit attached to m ~-l,2-1inked one in the side chains of the former mannan ,train [8,9], but lacking in the latter ones. We also reported in 'ecent papers that the serotype A-specific fl-1,2-1inked mannose mit in the mannans of C. albicans disappeared by cultivation )fthe cells under acidic pH [10] dida and several other yeasts. Thus, the detection of the circulating mannan antigen containing fl-l,2-1inkages in patients' sera by immunological procedures is important for the diagnosis of invasive candidiasis. Furthermore, several workers reported that the fl-1,2-linkage-containing side chains participate in adherence of C albicans cells to mammalian cells in the initial step of Candida infection [14,15]. Therefore, we tried to detect and characterize a fl-l,2-mannosyltransferase (ManTase) which is responsible for the biosynthesis of the serotype A specific epitope of C. albicans mannan. The biosynthetic pathway of the serotype A-specific side chain can be depicted as shown in Fig. 1 based on the structural analysis findings of C. albicans mannans [4]. Namely, the first transfer of the fl-l,2-linked mannose unit from GDP-mannose to the non-reducing terminal site of the ~-l,2-1inked mannotetraosyl side chain takes place by participation of the fl-l,2-ManTase I. However,...

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“…b-M are not only associated to the non-covalently bound molecules phosphopeptidomannan (PPM) and phospholipomannan (PLM), but also to other CWMPs either through O-or N-glycosylation. 58,60,61 The hyphal specific GPI-anchored protein Hwp1 contains b-M that are associated through O-glycosylation. 58 This protein is highly expressed under filamentous growth inducing conditions 62 and it was analyzed in this study.…”

Section: Cells (mentioning

confidence: 99%

The Cek1‑mediated MAP kinase pathway regulates exposure of α‑1,2 and β‑1,2‑mannosides in the cell wall ofCandida albicansmodulating immune recognition

et al. 2016

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Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide

Cited by 117 publications

References 33 publications

Lymphoproliferative and cytotoxic responses of human peripheral blood mononuclear cells to mannoprotein constituents of Candida albicans

Lymphoproliferative and cytotoxic responses of human peripheral blood mononuclear cells to mannoprotein constituents of Candida albicans

Detection of β‐1,2‐mannosyltransferase in Candida albicans cells

The Cek1‑mediated MAP kinase pathway regulates exposure of α‑1,2 and β‑1,2‑mannosides in the cell wall ofCandida albicansmodulating immune recognition

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