Immunochemical Study of Gangliosides at the Cell Surface of Sea Urchin Embryos

Михайлов, А. И.; Zvezdina, N. D.; Kocharov, Sergey L.; Мальченко, Л. А.; Buznikov, G. A.; Bergel'son, L. D.

doi:10.1111/j.1432-0436.1981.tb01102.x

Cited by 8 publications

(4 citation statements)

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“…As shown earlier, antisera to individual GI and G, erossreacted with these two gangliosides [5]. Therefore, in the present study, we used an antiserum to the total ganglioside fraction, anti-(G1 +G2) serum.…”

Section: Resultsmentioning

confidence: 80%

“…Using these antisera, certain changes in the localization of G1 and G2 were observed on the cell surface of S. intermedius embryos during the first cleavage divisions. A temporary increase of gangliosidc immunofluorescence occurred in the zone of interblastomer contacts [5]. It was also shown that eggs and early embryos of S. intermedius secreted gangliosides into the medium [7] and that these gangliosidcs lowcr the sensitivity of the embryos to some drugs including serotonin and its antagonists [5, 71. In this communication we describe the influence of antisera to gangliosides GI and G2 on the early embryogenesis of S. intermedius.…”

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confidence: 99%

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Gangliosides of sea urchin embryos

Zvezdina

Sadykova

Martynova

et al. 1989

European Journal of Biochemistry

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The influencc of antibodies to gangliosides of sea urchin Strongylocentrotus intermecliius eggs on early embryos of this species was studied. ?;-Globulins wcre isolated from rabbit anti-ganglioside serum by micropreparative electrophoresis. These y-globulins produced anomalies in the development of embryos pcrmeabilized in Triton X-100. The anomalies were not observed when anti-ganglioside y-globulins were added to the incubation medium together with gangliosides or when the permeabilized embryos were incubated with y-globulins of normal rabbit serum.Pretreatment of S. intevmedius embryos with serotonin, tryptamine or some other indole derivatives led to the disappearance of ganglioside determinants from the cell surface and sharply increased immunofluorescence within the cell. Such pretreatment of embryos increased the amount of cell-associated gangliosides more than threefold as compared to untreated embryos. Serotonin was shown to bind specifically to sea urchin gangliosides immobilizcd on octyl-Sepharose.These observations suggest that cell-surface gangliosides, after binding drugs, are internalized and that serotonin and its antagonists inhibit the transport of newly synthesized gangliosides to the cell-surface membrane.Gangliosides are known to play an essential and manifold role in cell interactions (for reviews see [l-31). These substances are localized predominantly in the outcr layer of the plasma membrane and can interact directly with exogenous physiologically active ligands or modulate their interaction with receptor proteins on the cell surface.Data obtained earlier by some of us suggested that gangliosides may be involved in the development of sea urchin embryos [4, 51. The eggs and early embryos of sea urchin are characterized by a very high (about 30 nmol/mg protein) content of gangliosides comparable to that in neural tissue of mammals [4].The chemical structure of two major gangliosides of sea urchin Strongylocentrofus intevmedius eggs and embryos was established. GI is N-glycoloylneuraminosyl-(a2 + 6) -glucosyl-rdmide; G 2 is GI with a sulfate ester group at C-8 of the terminal sialic acid [4, 61. Rabbit antisera against these gangliosides were obtained [4, 51. Using these antisera, certain changes in the localization of G1 and G2 were observed on the cell surface of S. intermedius embryos during the first cleavage divisions. A temporary increase of gangliosidc immunofluorescence occurred in the zone of interblastomer contacts [5]. It was also shown that eggs and early embryos of S. intermedius secreted gangliosides into the medium [7] and that these gangliosidcs lowcr the sensitivity of the embryos to some drugs including serotonin and its antagonists [5, 71. In this communication we describe the influence of antisera to gangliosides GI and G2 on the early embryogenesis of S. intermedius. We also show that 'prenervous' transmitters (see [S]) and some of their cytostatic analogs influencc the ganglioside content and compartmentalization in the embryonic cells. MATERIALS AND METHODS Sea urch...

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Section: Resultsmentioning

confidence: 80%

mentioning

confidence: 99%

Gangliosides of sea urchin embryos

Zvezdina

Sadykova

Martynova

et al. 1989

European Journal of Biochemistry

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“…Antisera were used after one or two injections of ganglioside. Activity and specificity of the antisera were tested by immunodiffusion (Mikhaylov et al, 1981).…”

Section: Methodsmentioning

confidence: 99%

Gangliosides Influence Experimental Influenza Virus Infection in Mice

Bukrinskaya

Petrova

Podchernyaeva

et al. 1989

Journal of General Virology

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“…The idea on the exchange with chemical signals between blastomeres suggested itself because of accumulation of various data on unusual concentrations of transmitters and related substances as gangliosides [109,110] and products of adenylate cyclase activity [103] in the contact area of blastomeres. Suggestion on the localization of transmitter receptors at the surface membrane of blastomeres [81][82][83] became impulse to elaborate the new concept.…”

Section: Protosynapsementioning

confidence: 99%