Immunochemical study of chromatin non-histone proteins

Ermekova, V M; Melkonyan, O. S.; Nanazashvili, Michael G.; Umansky, Samuil R.

doi:10.1007/bf00223303

Mol Cell Biochem

1984

DOI: 10.1007/bf00223303

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Immunochemical study of chromatin non-histone proteins

V M Ermekova

O. S. Melkonyan

Michael G. Nanazashvili

et al.

Abstract: Rabbit antibodies against rat thymus and liver chromatin are obtained. Antithymus IgG are found to interact only with homologous chromatin, while antiliver IgG interact with both liver and thymus chromatin. After preincubation of antiliver IgG with thymus chromatin the antibodies interact with homologous chromatin only. Thus chromatin of both organs contains tissue-specific proteins. Antiliver and antithymus IgG are used to investigate a distribution of tissue-specific and tissue-non-specific immunogenic prote… Show more

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Cited by 2 publications

References 26 publications

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Nuclear matrix and the regulation of gene expression: Tissue specificity

Getzenberg

1994

J of Cellular Biochemistry

135

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Tissue specific regulation of gene expression by a single transcription factor or group of transcription factors cannot be explained simply by DNA sequence alone. For example, in the same animal a particular transcription factor is capable of interacting with DNA in the nucleus of many different cell types, resulting in unique gene expressions despite the presence of a similar genome in all cells. Historically, these differences in response to a single type of factor within target tissues in the same animal have been suggested to occur through different alterations in chromatin structure. Recent, data has demonstrated that combinations of hormones and transcription factors working together may cooperatively play a role in the regulation of gene expression [Pearce and Yamamoto (1 993): Science 259:1161-I 1651. However, the molecular mechanisms of this tissue specific regulation of gene expression still remains largely unexplained. Current evidence suggests that in different cell types the interplay between the specific threedimensional organization of the genome and the structural components of the nucleus, the nuclear matrix, may accomplish the regulation of specific gene expression.

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Nuclear matrix and the regulation of gene expression: Tissue specificity

Getzenberg

1994

J of Cellular Biochemistry

135

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Tissue Specificity of the Hormonal Response in Sex Accessory Tissues Is Associated with Nuclear Matrix Protein Patterns

Getzenberg

Coffey

1990

Molecular Endocrinology

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The DNA of interphase nuclei have very specific three-dimensional organizations that are different in different cell types, and it is possible that this varying DNA organization is responsible for the tissue specificity of gene expression. The nuclear matrix organizes the three-dimensional structure of the DNA and is believed to be involved in the control of gene expression. This study compares the nuclear structural proteins between two sex accessory tissues in the same animal responding to the same androgen stimulation by the differential expression of major tissue-specific secretory proteins. We demonstrate here that the nuclear matrix is tissue specific in the rat ventral prostate and seminal vesicle, and undergoes characteristic alterations in its protein composition upon androgen withdrawal. Three types of nuclear matrix proteins were observed: 1) nuclear matrix proteins that are different and tissue specific in the rat ventral prostate and seminal vesicle, 2) a set of nuclear matrix proteins that either appear or disappear upon androgen withdrawal, and 3) a set of proteins that are common to both the ventral prostate and seminal vesicle and do not change with the hormonal state of the animal. Since the nuclear matrix is known to bind androgen receptors in a tissue- and steroid-specific manner, we propose that the tissue specificity of the nuclear matrix arranges the DNA in a unique conformation, which may be involved in the specific interaction of transcription factors with DNA sequences, resulting in tissue-specific patterns of secretory protein expression.

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Immunochemical study of chromatin non-histone proteins

Cited by 2 publications

References 26 publications

Nuclear matrix and the regulation of gene expression: Tissue specificity

Nuclear matrix and the regulation of gene expression: Tissue specificity

Tissue Specificity of the Hormonal Response in Sex Accessory Tissues Is Associated with Nuclear Matrix Protein Patterns

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