Immunochemical studies of SalmonellaDakar and SalmonellaTelaviv O-antigens (serogroup O:28)

Dziadziuszko, Halina; Kumirska, Jolanta; Muża, Sylwia; Czerwicka, Małgorzata; Lubecka, Emilia A.; Stepnowski, Piotr; Kunikowska, Danuta

doi:10.1111/j.1574-6968.2011.02431.x

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…It is likely that this terminal residue is also responsible for structural differences within the serogroup O28. Some aspects of the molecular basis for subdividing the O28 serogroup of the Kauffmann-White scheme into three subfactors O28, O28 1 and O28 2 has been recently explained [49]. Studies with monoclonal antibodies confirmed that the O28 1 —antigen specificity is attributed to the 3-substituted or 3,4-disubstituted α- d -Gal p NAc, which constitute the component of the main chain both S. Dakar and Salmonella Telaviv OPSs [49].…”

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confidence: 99%

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

et al. 2014

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Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16. ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep5Hex3HexN1Kdo1P1. Sugar and methylation analysis together with 1D and 2D 1H and 13C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→4)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. This composition was confirmed by mass spectrometry. The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H2O). Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.

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Section: Resultsmentioning

confidence: 99%

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

et al. 2014

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Serotypic Characterization of Salmonella Isolates in Meat and Poultry Products

Karmi

2014

Assiut Veterinary Medical Journal

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A total of 100 meat and poultry products samples were collected from shops and supermarkets in Aswan governorate; including 50 meat products samples and 50 poultry products. Samples were collected to determine the serotypes and antigenic structures of Salmonella isolates in meat and poultry products and to study the correlation between serotypes and type of meat. Salmonella was isolated by conventional methods and serotyping of Salmonella isolates was performed by slide agglutination method using antisera to Salmonella. It was observed that 16 % of samples were positive for Salmonella; 26% (13/50) in meat samples and 6% (3/50) in poultry samples. Serotypes of isolated Salmonella were Salmonella enteritidis 6.25% (1/16), Salmonella newland 18.75% (3/16), Salmonella Kentucky 12.5% (2/16), Salmonella heifa 6.25% (1/16), Salmonella bron 6.25% (1/16), Salmonella rissens 12.5% (2/16), Salmonella fortune 12.5% (2/16), Salmonella agona 6.25% (1/16), Salmonella hessarek 6.25% (1/16), Salmonella hoboken 6.25% (1/16), Salmonella veneziana 6. Meat and poultry products were contaminated with pathogenic Salmonella and the more prevalent serotypes were Salmonella newland, Salmonella rissens, Salmonella fortune, Salmonella Kentucky and the lesser prevalent isolates were Salmonella enteritidis, Salmonella heifa, Salmonella bron, Salmonella agona, Salmonella hessarek, Salmonella hoboken and Salmonella veneziana. Results showed that minced meat, kofta, liver and gizzard and sheeshtawook were more contaminated with Salmonellae. Meat and poultry products considered an important source of high risk serotypes of Salmonellae for human and hygienic measures should be undertaken to reduce contamination of meat and poultry products with Salmonella.

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Immunochemical studies of SalmonellaDakar and SalmonellaTelaviv O-antigens (serogroup O:28)

Cited by 2 publications

References 31 publications

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

Serotypic Characterization of Salmonella Isolates in Meat and Poultry Products

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