Immunochemical method for sulfasalazine determination in human plasma

Pastor-Navarro, Nuria; Gallego-Iglesias, Ester; Maquieira, Ángel; Puchades, Rosa

doi:10.1016/j.aca.2006.10.028

Cited by 35 publications

(17 citation statements)

References 13 publications

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“…Recently, the development of immunoassays capable of measuring multiple targets during a single test have been investigated, and these immunoassays are called broad-specificity, generic, group-or class-specific, or multi-analyte assays [68][69][70]. For a class-specific assay, selection of a hapten consisting of those features common to all the structures within the group to be targeted is required.…”

Section: Prediction Of Rational Hapten Designmentioning

confidence: 99%

Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review

Shen

Beier

et al. 2009

Analytica Chimica Acta

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Section: Prediction Of Rational Hapten Designmentioning

confidence: 99%

Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review

Shen

Beier

et al. 2009

Analytica Chimica Acta

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“…Due to the fact, that one of sulfonamides (i.e. STZ) reacts with the reducing sugars in honey [18], an extraction procedure based on SPE was required for samples analysis [26]. Honey samples (1 g) were spiked with SMX or SP at different concentrations in the range 0 -200 ng/mL.…”

Section: Honey Samples Treatment and Solid Phase Extractionmentioning

confidence: 99%

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

et al. 2010

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In this work an electrochemical immunoassay, based on a direct competitive assay, was developed using magnetic beads as solid phase and carbon screen-printed arrays as transducers for the detection of sulfonamides in food matrices such as honey. Magnetic beads coated with protein A were modified by immobilisation of specific antibodies and then the competition between the target analyte and the corresponding analyte-labelled with an enzyme was carried out; after the immunosensing step, beads were captured by a magnet onto the working surfaces of a screenprinted eight-electrodes array for a multiple electrochemical detection. Screen-printed eight-electrodes arrays were chosen as transducers due to the possibility to repeat multiple analysis and to test different samples simultaneously. Alkaline Phosphatase (AP) was used as enzyme label and Differential Pulse Voltammetry (DPV) as fast electrochemical technique. Calibration curves demonstrate that the developed electrochemical immunoassay was able to detect this class of drugs in standard solutions at low concentrations (ng/mL levels). The short incubation times (25 min) and the fast electrochemical measurement (10 sec) make of these systems a possible alternative to classic ELISA tests. The developed electrochemical immunoassay was coupled to Solid Phase Extraction (SPE) for the analysis of these compounds in a food matrix such as honey.

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“…Sulfasalazine 5-[4-(2-pyridylsulfamoyl)phenylazo]salicylic acid was designed by Svartz in 1942 by combining an antibiotic sulfapyridine with an anti-inflammatory agent 5-aminosalicylic acid through an azo bond [1]. Sulfasalazine is a bright-yellow or brownish-yellow fine powder, practically insoluble in water, very slightly soluble in alcohol, practically insoluble in methylene chloride, but well soluble in dilute solutions of alkali hydroxides [2].…”

Section: Introductionmentioning

confidence: 99%

“…Different analytical methods were applied for the determination of sulfasalazine in pharmaceutical preparations and biological fluids. For the assessment of sulfasalazine in several biological matrices, an enzyme-linked immunosorbent assay method based on polyclonal antibodies was developed and characterized [1]. Ultraviolet (UV) spectrophotometric method was applied for the quantitative determination of sulfasalazine in tablet dosage form [5].…”

Section: Introductionmentioning

confidence: 99%

TLC—Densitometric Determination of Sulfasalazine and Its Possible Impurities in Pharmaceutical Preparations

Kwiecień

Piątek

Żmudzki

et al. 2015

Acta Chromatographica

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Summary.A simple, selective, and sensitive thin-layer chromatographic-densitometric method has been developed for the determination of sulfasalazine besides its possible impurities in pharmaceutical preparations. The mobile phase was composed of ethyl acetate-methanol-ammonia 25% 10:7:3 (v/v/v), and the stationary phase was aluminum plates precoated with silica gel 60 F 254 that enabled to obtain well resolved peaks of sulfasalazine and its impurities. The developed chromatograms were analyzed densitometrically at λ = 360 nm. R F values and ultraviolet (UV) spectra were used to identify the compounds. The developed method is highly sensitive (limit of detection [LOD] = 17.11 ng spot −1 , limit of quantitation [LOQ] = 51.84 ng spot −1 ), precise (relative standard deviation [RSD] = 1.43%-4.28%), and accurate (RSD = 1.64%-4.27%). The linearity of the method was checked within the range 20-120 ng spot −1 . The method was successfully applied for the determination of sulfasalazine in pharmaceutical preparations besides its impurities. The structures of impurities present in the standard substance and in pharmaceutical preparations were established by ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS) technique.

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Immunochemical method for sulfasalazine determination in human plasma

Cited by 35 publications

References 13 publications

Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review

Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review

Development of an Electrochemical Immunoassay Based on the Use of an Eight‐Electrodes Screen‐Printed Array Coupled with Magnetic Beads for the Detection of Antimicrobial Sulfonamides in Honey

TLC—Densitometric Determination of Sulfasalazine and Its Possible Impurities in Pharmaceutical Preparations

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