Immunochemical identification of a new embryo-specific?-globulin in the blood serum of human embryos, fetuses, and neonates

Tatarinov, Yu. S.; Masyukevich, V. N.

doi:10.1007/bf00808980

Cited by 21 publications

(29 citation statements)

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“…The human CEA family, recognized by CD66 and CD67 antibodies, consists of two major subfamilies, one termed the CEA subgroup, containing CEA, NCA, biliary glycoprotein (41), CGM1 (CGM1a, CGM1b, and CGM1c) (24,42), CGM2, CGM6, and CGM7 (CGM ϭ CEA gene family member). The second subfamily consists of the pregnancy-specific glycoproteins (43)(44)(45).…”

Section: Microbiology: Chen and Gotschlichmentioning

confidence: 99%

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins

Chen

Gotschlich

1996

Proc. Natl. Acad. Sci. U.S.A.

160

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Section: Microbiology: Chen and Gotschlichmentioning

confidence: 99%

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins

Chen

Gotschlich

1996

Proc. Natl. Acad. Sci. U.S.A.

160

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“…Originally, they were discovered independently by Tatarinov and Masyukevich (1970) and Bohn (1971) as one of the pregnancy-specific proteins. These proteins can be produced in human intestinal tissue (Shupert and Chan, 1993), T lymphocytes (Wu et al, 1993) and male reproductive tract (Richardson et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

An enzyme immunoassay for pregnancy-specific β-1 glycoprotein (SP1), employing monoclonal antibodies

Lee

Kim²,

Chung³

1996

Exp Mol Med

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Two-site binding enzyme immunoassay for pregnancy-specific -1 glycoprotein (SP1) has been developed using two monoclonal antibodies which can recognize all the three forms of SP1 from human sera. This assay system detects a minimal concentration of 1.25 ng/ml, and shows a linear dose-response curve at a range between 1.4 and 45.0 ng/ml. The recovery rates ranged from 90.0% to 113.3%, and intra-assay coefficients of variation were 15.8% and 8.7% for two different samples. When compared with a commercial EIA kit, this assay system had high correlation coefficient (r = 0.92) but with somewhat lower values. K e y w o r d s ; enzyme immunoassay, monoclonal antibody, pregnancy-specific β-1 glycoprotein (SP1)

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“…At term, they represent the major placental protein species secreted into the maternal blood (Lin et al, 1974). These proteins were originally discovered by Tatarinov and Masyukevich (1970) and Bohn (1971) and are heterogeneous with respect to their sizes (Watanabe and Chou, 1988). Using antisera, PSGs have been located to the syncytiotrophoblast (Horne et al 1976).…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal expression of pregnancy‐specific glycoprotein gene rnCGM1 in rat placenta

Rebstock

Lucas

Weiß

et al. 1993

Developmental Dynamics

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As a basis towards a better understanding of the role of the pregnancy-specific glycoprotein (PSG) family in the maintenance of pregnancy, detailed investigations are described on the expression of a recently identified rat PSG gene (rnCGMl) at the mRNA and protein levels. Using specific oligonucleotide primers, rnCGMl transcripts were identified after reverse transcription, polymerase chain reaction, and hybridization with a radiolabelled, internal oligonucleotide. Transcripts were only found in significant amounts in placenta. In situ hybridization visualized rnCGM1 transcripts at day 14 post coitum (P.c.), in secondary trophoblast giant cells and in the spongiotrophoblast. Only those secondary giant cells lining the maternal decidua were positive. In contrast, primary giant cells did not contain rnCGMl mRNA. At day 18 P.c., rnCGMl transcripts were almost exclusively detectable in the spongiotrophoblast. No rnCGMl transcripts were found in rat embryos of these two developmental stages. Rabbit antisera were generated against the amino-terminal immunoglobulin variable-like domain and against a synthetic peptide containing the last 13 carboxy-terminal amino acids of rnCGMI. Both antisera recognized a 124 kDa protein in day 18 rat placental extracts as identified by Western blot analysis. The anti-peptide antiserum recognized a 116 kDa protein in the serum of a 14 day p.c. pregnant rat that is absent from the sera of non-pregnant females. Taken together, these results confirm exclusive expression of rnCGMl in the rat trophoblast, but unlike human PSG, negligible or no expression is found in other organs, such as fetal liver or salivary glands, indicating a more specialized function of rnCGM1. Its spatiotemporal expression pattern is conducive with a potential role of PSG in protecting the fetus against the maternal immune system andlor in regulating the invasive growth of trophoblast cells. 0 1993 Wiley-Liss, Inc.

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Immunochemical identification of a new embryo-specific?-globulin in the blood serum of human embryos, fetuses, and neonates

Cited by 21 publications

References 1 publication

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins

An enzyme immunoassay for pregnancy-specific β-1 glycoprotein (SP1), employing monoclonal antibodies

Spatiotemporal expression of pregnancy‐specific glycoprotein gene rnCGM1 in rat placenta

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