Immunochemical detection of S-100 protein in non-nervous structures of the rabbit eye

Michetti, Fabrizio; Rende, Mario; Calogero, Giuseppe; Dell'Anna, Elisabetta; Cocchia, Domenico

doi:10.1016/0006-8993(85)90604-3

Cited by 19 publications

(8 citation statements)

References 21 publications

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“…B-and T-cells were YFPϪ in all locations (data not shown). Dendritic cells have been previously reported to express S100B (Cocchia et al, 1981Michetti et al, 1983Michetti et al, , 1985. In our material, however, many YFPϩ dendritic cells were not labeled by a polyclonal antibody to S100B, suggesting that the relationship between expression of the endogenous gene and the transgene is an artifact of transgenesis.…”

Section: Resident Macrophages In Muscles and Peripheral Nerves Exprescontrasting

confidence: 57%

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Zuo¹,

Lubischer²,

Kang³

et al. 2004

J. Neurosci.

141

158

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To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.

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Section: Resident Macrophages In Muscles and Peripheral Nerves Exprescontrasting

confidence: 57%

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Zuo¹,

Lubischer²,

Kang³

et al. 2004

J. Neurosci.

141

158

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“…Phakomatous choristoma cells also synthesize lens‐specific proteins and the anti‐lens protein antibodies α, β, and γ crystalline, which can be detected immunohistochemically 8 . The immunohistochemical profile of epithelial cells, which includes expression of vimentin, S‐100 protein, and negative staining for cytokeratins and epithelium membrane antigen, are identical to that of mature lens epithelium 9 …”

Section: Discussionmentioning

confidence: 92%

Phakomatous choristoma of the eyelid: a report of a case

et al. 2004

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Phakomatous choristoma is a rare congenital hamartoma of lens tissue. It presents in newborns or young infants as a subcutaneous mass in the medial lower eyelid. We present a case of phakomatous choristoma recently identified among old consultation files at Children's Hospital in Boston. Our case illustrates unique histological features of this entity which has to be included in differential diagnosis of skin lesions of the inferonasal eyelid.

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“…It is also observed in the non-pigmented ciliary epithelium, in the posterior epithelium of the iris, in Schwann cells along ciliary nerves, and in a number of as yet unidentified cell types in the iris stroma and ciliary body (Iwanaga et al 1985). Immunochemical studies have suggested, that S-100 proteinpositive cells might be present in the cornea and in the lens (Michetti et al 1985). Most malignant melanomas of the uveal tract also express S-100 protein (Nakajima et al 1982b;Cochran et al 1983).…”

mentioning

confidence: 98%

S‐100 protein in retinoblastoma revisited

Kivelä

Tarkkanen

1986

Acta Ophthalmologica

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Fifty formalin-fixed and paraffin-embedded retinoblastoma specimens were studied for the presence of S-100 protein in malignant tumour cells and in reactive glial cells. Its distribution in four normal human eyeglobes enucleated because of an orbital tumour was also studied. The laboratory method was a sensitive immunohistochemical staining procedure, and sections from human brain served as controls. Three anti-S-100 protein antisera were used to determine whether previously reported discrepancies could be related to differences in antibody specificity, but all gave identical results. In morphologically normal human retina, a strong reaction for S-100 protein was seen in astrocytes, and a faint, inconsistent positivity was detected in Müller's cells. All intraocular retinoblastomata studied contained cells positive for S-100 protein, which seemed to be reactive glial cells derived from infiltrated retina. Undifferentiated malignant retinoblastoma cells, as well as differentiated tumour cells in Flexner-Wintersteiner rosettes were negative for S-100 protein. Differentiated tumours appeared to contain more S-100 protein-positive glial cells than undifferentiated tumours, but this may be related to their different growth rates. The results do not support the theory that retinoblastoma cells express glial properties.

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Immunochemical detection of S-100 protein in non-nervous structures of the rabbit eye

Cited by 19 publications

References 21 publications

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Phakomatous choristoma of the eyelid: a report of a case

S‐100 protein in retinoblastoma revisited

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