Immunochemical Characterization of Tomato Peel and Pulp Extracts

Carnés, Jerónimo; López-Matas, M. Ángeles; Ferrer, A.; Larramendi, Carlos H.; Huertas, Jaqueline Mena; Casanovas, Miguel; Fernández-Caldas, Enrique

doi:10.1016/j.jaci.2005.12.1192

Cited by 3 publications

(3 citation statements)

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“…Canary tomato variety was selected due to its higher protein content compared to other tomato varieties [ 11 ] and to its high sensitivity in SPT, inducing the largest wheals when compared with other varieties [ 16 ]. There are no studies comparing manufactured prick tests and prick-prick tests with fresh tomatoes, but our results replicate those found by several authors with different fruits (apple and peach for example) [ 3 , 4 , 7 ].…”

Section: Discussionmentioning

confidence: 99%

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Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method

Ferrer

Huertas²,

Larramendi

et al. 2008

Clin Mol Allergy

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BackgroundCommercial available skin prick test with fruits can be negative in sensitized or allergic patients due to a reduction in biological activity during the manufacturing process. Prick-prick tests with fresh foods are often preferred, but they are a non-standardized procedure. The usefulness of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method in the diagnosis of tomato sensitization has been analyzed.The objective of the study was to assess the potential diagnostic of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method.MethodsTwo groups of patients were analyzed: Group I: 26 individuals reporting clinical symptoms induced by tomato contact or ingestion. Group II: 71 control individuals with no symptoms induced by tomato: 12 of them were previously skin prick test positive to a tomato extract, 39 were atopic and 20 were non-atopic. All individuals underwent prick-prick with fresh ripe peel Canary tomatoes and skin prick tested with freeze-dried peel and pulp extracts obtained from peel and pulp of Canary tomatoes at 10 mg/ml. Wheal sizes and prick test positivity (≥ 7 mm2) were compared between groups.ResultsIn group I, 21 (81%) out of 26 patients were prick-prick positive. Twenty patients (77%) had positive skin prick test to peel extracts and 12 (46%) to pulp extracts. Prick-prick induced a mean wheal size of 43.81 ± 40.19 mm2 compared with 44.25 ± 36.68 mm2 induced by the peel extract (Not significant), and 17.79 ± 9.39 mm2 induced by the pulp extract (p < 0.01).In group II, 13 (18%) out of 71 control patients were prick-prick positive. Twelve patients (all of them previously positive to peel extract) had positive skin prick test to peel and 3 to pulp. Prick-prick induced a mean wheal size of 28.88 ± 13.12 mm2 compared with 33.17 ± 17.55 mm2 induced by peel extract (Not significant), and 13.33 ± 4.80 mm2 induced by pulp extract (p < 0.05 with peel extract and prick-prick).ConclusionCanary peel tomato extract seems to be as efficient as prick-prick tests with ripe tomatoes to diagnose patients sensitized to tomato. The wheal sizes induced by prick-prick and peel extracts were very similar and showed a high correlation coefficient.

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Section: Discussionmentioning

confidence: 99%

“…The Canary Island tomato variety was selected due to its frequent consumption in Spain [ 10 ] and its high allergenicity [ 11 ]. Ripe tomatoes were purchased at a local market 2 days before preparing the extract, washed in bidistilled water and carefully peeled.…”

Section: Methodsmentioning

confidence: 99%

Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method

Ferrer

Huertas²,

Larramendi

et al. 2008

Clin Mol Allergy

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“…The Canary Island tomato variety was selected for the study because of its frequent consumption in Spain [1] and its high allergenicity [29]. Ripe tomatoes were purchased at a local market, washed in distilled water and carefully peeled; 217.33 g of peel and 360.8 g of pulp were used to manufacture two different extracts of peel and pulp following previously described methods [30].…”

Section: Tomato Extractsmentioning

confidence: 99%

Sensitization to tomato peel and pulp extracts in the Mediterranean Coast of Spain: prevalence and co‐sensitization with aeroallergens

Larramendi

Ferrer

Huertas³

et al. 2007

Clin Experimental Allergy

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The prevalence found of skin test sensitivity to tomato is high. Peel extracts detected most of the sensitized subjects. Most of the sensitized subjects were asymptomatic and some patients reported symptoms without skin test sensitivity. Positive subjects were very frequently sensitized to pollens, suggesting allergen cross-reactivity. Regional differences may exist, possibly related to the pattern of sensitization to cross-reacting pollens.

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Emerging Allergens and the Future

Achouri

Boye

2010

Allergen Management in the Food Industry

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Immunochemical Characterization of Tomato Peel and Pulp Extracts

Cited by 3 publications

References 0 publications

Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method

Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method

Sensitization to tomato peel and pulp extracts in the Mediterranean Coast of Spain: prevalence and co‐sensitization with aeroallergens

Emerging Allergens and the Future

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