Immunochemical Characterization of the Capsular Polysaccharide of Azospirillum irakense KBC1

Fedonenko, Yu. P.; Burygin, Gennady L.; Попова, И. А.; Sigida, Elena N.; Surkina, A. K.; Zdorovenko, Evelina L.; Коннова, С. А.

doi:10.1007/s00284-013-0346-1

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…A lipopolysaccharide (LPS) having an O-specific polysaccharide chain (OPS) called O antigen and capsular polysaccharide (K antigen) of Azospirillum , which is an extracellular form of LPS [ 8 – 9 ], are important for the interaction between bacteria and host plants. The cell-surface polysaccharides of Azospirillum are involved in overcoming of unfavorable conditions, including survival of bacteria under salinity [ 10 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

Sigida¹,

Fedonenko²,

Shashkov³

et al. 2016

Beilstein J. Org. Chem.

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SummaryAn O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide isolated by the phenol–water extraction from the halotolerant soil bacteria Azospirillum halopraeferens type strain Au4. The polysaccharide was studied by sugar and methylation analyses, selective cleavages by Smith degradation and solvolysis with trifluoroacetic acid, one- and two-dimensional 1H and 13C NMR spectroscopy. The following masked repeating structure of the O-specific polysaccharide was established: →3)-α-L-Rhap2Me-(1→3)-[β-D-Glcp-(1→4)]-α-D-Fucp-(1→2)-β-D-Xylp-(1→, where non-stoichiometric substituents, an O-methyl group (~45%) and a side-chain glucose residue (~65%), are shown in italics.

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Section: Introductionmentioning

confidence: 99%

Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

Sigida¹,

Fedonenko²,

Shashkov³

et al. 2016

Beilstein J. Org. Chem.

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“…Their ability to induce deformations of root hairs of wheat was shown for the first time (Yegorenkova et al, 2001). Novel information was obtained on the primary structures of repetitive PS units in surface lipopolysaccharides (LPSs) and capsular PSs of more than 40 Azospirillum strains of various origins (Fedonenko et al, 2013(Fedonenko et al, , 2015. In several strains isolated from plant roots on different continents, molecular mimicry of bacterial surface glycopolymers owing to the presence of identical or structurally similar repeating units in an O-specific PS (O-PS) was observed.…”

Section: The Key Results Obtained At the Ibppm Ras In Studies Of Azospirillum Bacteria As Model Objectsmentioning

confidence: 99%

The Collection of Rhizosphere Microorganisms: its importance for the study of associative plant-bacterium interactions

Turkovskaya

Голубев

2020

Vestn. VOGiS

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Microbial culture collections are very important components of biological science. They provide researchers with material for studies and preserve biological resources. One such collection is the Collection of Rhizosphere Microorganisms, kept at the Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences, Saratov (IBPPM). Its activity is primarily directed toward the isolation and preservation of microorganisms from the plant root zone. The international research interest in microorganisms from this ecological niche is not waning, because they are very important for plant growth and development and, consequently, for plant breeding. The group of bacteria with properties of significance for plants has been given the name “plant-growth-promoting rhizobacteria” (PGPR). This group includes nitrogen-fixing soil alpha-proteobacteria of the genus Azospirillum, which form the core of the IBPPM collection. First discovered by Brazilian scientists in the 1970s, azospirilla are now a universally recognized model object for studying the molecular mechanisms underlying plant-bacterium interactions. The broad range of useful properties found in these microorganisms, including the fixation of atmospheric nitrogen, production of phytohormones, solubilization of phosphates, control of pathogens, and formation of induced systemic resistance in the colonized plants, make these bacteria an all-purpose tool that has been used for several decades in basic and applied research. This article reviews the current state of Azospirillum research, with emphasis on the results obtained at the IBPPM. Scientific expeditions across the Saratov region undertaken by IBPPM microbiologists in the early 1980s formed the basis for the unique collection of members of this bacterial taxon. Currently, the collection has more than 160 Azospirillum strains and is one of the largest collections in Europe. The research conducted at the IBPPM is centered mostly on the Azospirillum structures involved in associative symbiosis with plants, primarily extracellular polysaccharide-containing complexes and lectins. The development of immunochemical methods contributed much to our understanding of the overall organization of the surface of rhizosphere bacteria. The extensive studies of the Azospirillum genome largely deepened our understanding of the role of the aforesaid bacterial structures, motility, and biofilms in the colonization of host plant roots. Of interest are also applied studies focusing on agricultural and environmental technologies and on the “green” synthesis of Au, Ag, and Se nanoparticles. The Collection of Rhizosphere Microorganisms continues to grow, being continually supplemented with newly isolated strains. The data presented in this article show the great importance of specialized microbial culture repositories, such as the IBPPM collection, for the development and maintenance of the microbial research base and for the effective solution of basic and applied tasks in microbiology.

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“…Ранее нами выделен и охарактеризован по химическому составу ЛПБК из капсульного материала A. irakense КВС1. Выяснено, что ЛПБК в капсуле является экстраклеточной формой ЛПС, и изолированный из него полисахарид идентичен О-специфическому полисахариду (ОПС) из ЛПС KBC1 и построен из разветвленных гексасахаридных повторяющихся звеньев, содержащих остатки D-галактозы, L-рамнозы и D-маннозы [12]. С целью установления вклада белковой составляющей в реализацию иммуностимулирующей активности гликополимера капсулы азоспирилл ЛПБК KBC1 обрабатывали протеиназой К, после чего оценивали его иммуномодулирующий эффект.…”

Section: результаты и их обсуждениеunclassified

“…Различное влияние протеиназной обработки на биологическую активность ЛПБК КВС1 in vivo и in vitro можно объяснить тем, что ЛПС [6] и ЛПБК A. irakense КВС1 являются активными индукторами синтеза оксида азота в спленоцитах мышей. Установлено, что удаление белковой компоненты из ЛПБК КВС1 приводило к снижению продукции NO в 2 раза по сравнению с нативным препаратом [12]. Значительная стимуляция синтеза NO может отражать развитие токсигенной реакции макроорганизма на эндотоксин, что может служить объяснением наблюдаемой корреляции между результатами по индукции NO и значениями LD 50 для ЛПБК КВС1 и ЛПБК КВС1 ПрК .…”

Section: результаты и их обсуждениеunclassified

Glycopolymers from Bacteria of the Genus Azospirillum as Promising Antagonists of Classic Endotoxins

Surkina¹,

Коннова²,

Fedonenko³

et al. 2013

CBE

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Проведено сравнительное исследование агонистических и антагонистических свойств, а также токсичности гликополимеров бактерий рода Azospirillum. Впервые для азоспирилл показано, что их гликаны обладают антагонистическими свойствами в отношении классических эндотоксинов. Установлено, что существенный вклад в реализацию этой активности вносят отдельные жирные кислоты в составе липидов А. Показано влияние конформации исследованных гликанов на их летальную токсичность и ингибирующее действие в отношении эндотоксина Escherichia coli O55:B5. Среди изученных препаратов наивысшую антагонистическую активность проявлял липополисахарид бактерий A. lipoferum Sp59b.

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Immunochemical Characterization of the Capsular Polysaccharide of Azospirillum irakense KBC1

Cited by 8 publications

References 16 publications

Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

The Collection of Rhizosphere Microorganisms: its importance for the study of associative plant-bacterium interactions

Glycopolymers from Bacteria of the Genus Azospirillum as Promising Antagonists of Classic Endotoxins

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