Immunochemical characterization of seed proteins of some species of the genusArachis L.

Klozová, Eva; Turková, Věra; Smartt, J.; Pitterová, Květa; Švachulová, Jiřina

doi:10.1007/bf02902103

Cited by 15 publications

(9 citation statements)

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“…The protein profiles in the species/accessions representing seven different sections of genus Arachis broadly appear similar, corroborating earlier findings of conservative protein profile patterns in the genus Arachis (Klozova et al, 1983a(Klozova et al, , 1983bSingh et al, 1991). These differences and similarities observed support the broad sub-classification of genus Arachis proposed by Gregory et al (1980) and Krapovickas (1990).…”

Section: Discussionsupporting

confidence: 79%

“…Triseminalae and Extranervosae are the farthest (Table 3). These relationships support the earlier conclusions of Cherry (1975) based on electrophoretic protein and enzyme profile, and of Neucere & Cherry (1975) and Klozova et al (1983aKlozova et al ( , 1983b) based on immunochemical similarity. The accessions belonging to sections phylogenetically closer to section Arachis have greater prospects in genetic improvement of groundnut than distant ones.…”

Section: Discussionsupporting

confidence: 78%

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Phylogenetic relationships in the genus Arachis based on seed protein profiles

1993

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Seed protein profiles of 19 accessions representing seven sections of the genus Arachis were studied using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The general profile showed appreciable homology between these taxa, supporting their classification based on morphology and cross-compatibility relationships. The accessions of section Arachis expressed a high variation confirming inferences from earlier studies. Variation between accessions of a species is limited. Accessions of the section Ambinervosae and Caulorhizae formed one cluster and accessions of sect. Erectoides and Procumbensae formed another. Whereas the representative accessions of sect. Triseminalae and Extranervosae formed two independent clusters. Using the percentage of dissimilarity in electrophoretic bands as a statistical genetic distance between accessions, sect. Arachis (containing the cultivated groundnut, A. hypogaea) is phylogenetically closest to sect. Erectoides followed by Procumbensae, Ambinervosae, Caulorhizae, Triseminalae and Extranervosae, respectively.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 78%

Phylogenetic relationships in the genus Arachis based on seed protein profiles

1993

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“…This is because crossing between these diploid species results in the production of a fully fertile diploid F 1 hybrid (Stalker & Wynne, 1979;Singh & Moss, 1984a) that can propagate without any necessity for doubling of chromosomes to regain fertility. Secondly A. cardenasii has been found immunologically distant from A. hypogaea (Klozová et al, 1983).…”

Section: Congruency Of Various Hypotheses With Resultsmentioning

confidence: 99%

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Singh

Smartt

1998

Genetic Resources and Crop Evolution

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Arachis hypogaea, the cultivated groundnut is a tetraploid with an AABB genomic constitution. The available literature on the origin of groundnut reveals that there is general agreement that the cultivated groundnut has evolved from the wild tetraploid species A. monticola, with which it crosses freely to produce fertile hybrids. However, the issue of actual diploid ancestors of A. monticola is still unresolved. Both cytogenetic and molecular evidences support A. duranensis being the most probable progenitor and donor of the A genome to A. hypogaea. For the B genome, the cytogenetic evidence suggests A. batizocoi to be the most probable progenitor, but the RFLP banding pattern indicates that A. batizocoi is more distantly related to A. hypogaea than other species of section Arachis. RFLP banding pattern indicates A. ipaensis to be one of the closest species to A. hypogaea and the possible donor of the B genome. The present article critically analyzes the available data, which suggests that until an amphidiploid is produced synthetically between A. duranensis A. ipaensis and crossed successfully with A. hypogaea to produce a fertile hybrid, this issue would remain unresolved. A. batizocoi would remain the most probable donor of the B genome because of its directly demonstrable cytogenetic affinity.

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“…monticola (Smartt et al, 1978 ;Smartt, 1985) . Attempts have been made to use isozymes and seed proteins of Arachis species to establish species interrelationships (Cherry, 1975(Cherry, , 1977Klozova et al ., 1983aKlozova et al ., , 1983b . Cherry (1975Cherry ( , 1977 showed that the diploid Arachis species belonging to the section Axenomorphae and the series Annuae and Perennes demonstrated broadly a similar native arachin pattern with that of A .…”

Section: Introductionmentioning

confidence: 99%

“…Klozova et al . (1983aKlozova et al . ( , 1983b indicated from their immuno-electrophoresis and double diffusion studies with seed proteins of Arachis species that strong relationship exists between A .…”

Section: Introductionmentioning

confidence: 99%

The probable genome donors to Arachis hypogaea L. based on arachin seed storage protein

Krishna

Mitra

1988

Euphytica

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Interrelationships among six diploid species (A. chacoense, A. villosulicarpa, A . batizocoi, A . correntina, A . duranensis and A . cardenasii), tetraploid wild groundnut A . monticola and four accessions of A . hypogaea were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of total seed proteins and arachin immunoprecipitates . Arachin in A . monticola and A . hypogaea cv . Trombay Groundnut 9 (TG-9) was composed of four acidic subunits (47 .5 kd, 45 .1 kd, 42 .6 kd and 41 .2 kd) and one major basic subunit (21 .4 kd) . The arachin subunit pattern in cv . Spanish Improved (SP) and TG-1 was almost similar to the pattern observed in A . monticola with the exception that the SP lacked the 41 .2 kd and TG-1 lacked the 42.6 kd acidic subunits of A. monticola. Seed extracts of all diploid species studied reacted with the anti-arachin antibodies raised against SP arachin . The electrophoretic analysis of immunoprecipitates of diploid species showed a range of acidic subunits from 46 .2 kd to 41 .2 kd and one or two basic subunits of 21 .4 kd and 20 .2 kd . None of the diploid species showed the 47 .5 kd subunit found in A . monticola or A . hypogaea . Of the diploid species, A. duranensis and A . cardenasii demonstrated two acidic subunits of 45 .1 kd and 41.2 kd and a basic subunit of 21 .4 kd as found in A . monticola . Likewise A . batizocoi showed two acidic subunits of 45 .1 kd and 42 .6 kd and the basic subunit of 21 .4 kd as was observed in A . monticola. Based on electrophoretic data, our research supports the earlier conclusion that the probable genome donors to A . monticola are A. batizocoi and A . duranensis or A . cardenasii.

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Immunochemical characterization of seed proteins of some species of the genusArachis L.

Cited by 15 publications

References 14 publications

Phylogenetic relationships in the genus Arachis based on seed protein profiles

Phylogenetic relationships in the genus Arachis based on seed protein profiles

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The probable genome donors to Arachis hypogaea L. based on arachin seed storage protein

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