Immunochemical characterization of anti-H monoclonal antibodies obtained from a mouse immunized with human saliva

Nakajima, Tamiko; Yazawa, Shin; Miyazaki, Seiko; Furukawa, Ken

doi:10.1016/0022-1759(93)90165-4

Cited by 22 publications

(29 citation statements)

References 18 publications

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“…The specificity of MAb 3A5 has been demonstrated to be specific for the H Type 2 structure using a series of synthetic oligosaccharides (Liu et al 1996a (Nakajima et al 1993). UEA-I agglutinin conjugated with horseradish peroxidase (HRP) was purchased from EY Laboratories (San Mateo, CA).…”

Section: Antibodies and Lectinmentioning

confidence: 99%

“…In the present study, we compared the stainings of the H antigens in cell types of human submandibular gland by two anti-H MAbs (MAb 3A5 and MAb 1E3, which are specific for H Type 2 and for H Types 1-4, respectively) (Nakajima et al 1993;Liu et al 1996a). We developed a competitive inhibition method against H Type 2 by absorption of MAb 1E3 with synthetic H Type 2-albumin complex or red blood cell ghosts and we obtained MAb 1E3 fairly specific for H Type 1 antigen.…”

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confidence: 99%

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Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

Liu

Fujitani

Koda

et al. 1998

J Histochem Cytochem.

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We have examined the immunohistochemical distribution of H Type 1 and of H Type 2 substances of the ABO blood group system in human submandibular gland using either of the two anti-H monoclonal antibodies MAb 1E3 and MAb 3A5. MAb 3A5 was specific for H Type 2, and MAb 1E3 reacted with each of H Type 1-H Type 4 artificial antigens. We have developed a competitive inhibition method against H Type 2 and have obtained MAb 1E3, which is fairly specific for H Type 1 under certain conditions. Mucous cells from secretors were strongly stained by 1E3 and weakly by 3A5, whereas those from nonsecretors showed no reaction with 1E3 and 3A5. Serous cells from both secretors and nonsecretors were stained neither by 1E3 nor by 3A5. Striated and interlobular duct cells were strongly stained by 1E3 and by 3A5, regardless of the secretor status. These results indicated that the expressions of the H Type 1 and H Type 2 in different cell types of the submandibular gland were controlled by different genes. In addition, we have determined the acceptor specificity of two alpha(1,2)fucosyltransferases (H and Se enzymes) after transient expressions of the FUT1 and FUT2 in COS7 cells, and found that the H enzyme activity was similar for both Type 1 and Type 2 precursors, and that Se enzyme activity with the Type 1 precursor was higher than that with the Type 2 precursor. Expression of the H Type 1 antigen in mucous cells was found to be dependent on the Se gene, whereas expressions of the H Type 1 and H Type 2 antigens in striated and interlobular duct cells were dependent on the H gene. (J Histochem Cytochem 46:69-76, 1998)

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Section: Antibodies and Lectinmentioning

confidence: 99%

mentioning

confidence: 99%

Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

Liu

Fujitani

Koda

et al. 1998

J Histochem Cytochem.

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“…(25) In summary, we have generated an anti-BG-H2 antibody L9E10, discovered the expression of BG-H2 antigen in colon CSCs, and demonstrated the inhibition effect of MAb L9E10 on colon cancer NSY cell migration and invasion. Despite the fact that antibodies to BG-H2 have been reported, (26)(27)(28)(29)(30) some of the antigen was identified based only on agglutination assays of red blood cells and Ulex Europaeus lectin assays. (28)(29)(30) In this study, we used a carbohydrate array to identify the MAb L9E10 binding antigen as a BG-H2.…”

Section: Resultsmentioning

confidence: 99%

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

Wang

Gildersleeve

et al. 2010

Hybridoma

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The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal antibody designated as MAb L9E10. The binding antigen of MAb L9E10 was identified as blood group (BG) H2 antigen using carbohydrate array and erythrocyte agglutination assays. In immunofluorescence study, we found that BG-H2 was expressed on the surfaces of both colon cancer stem cells and their differentiated progeny. In a functional study, we observed that MAb L9E10 inhibited tumor cell migration and invasion at a concentration of 10 mg/mL in vitro. This result suggests that MAb L9E10 could be used to study cancer biology, particularly cancer stem cell biology. In addition, it is potentially useful for studying gastric diseases caused by Helicobacter pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. Finally, MAb L9E10 is an ideal biological reagent for identifying Bombay blood type in which erythrocytes have no BG-H2 antigen expression.

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“…Nakajima et al [14] have reported that anti-H 1E3 reacted with the terminal disaccharide structure (Fucal-2Gal), that is, reacted with the type 1, 2, 3 and 4 chains of the H antigen but not with Leb. As shown in figures la and 2a, anti-H 1E3 reacted with both H type 1 and H type 2 chains with a lower reactivity with H type 2, at about 40% that with H type 1, but did not react with the Lea or Leb.…”

Section: Resultsmentioning

confidence: 99%

“…Anti-Lea and anti-Leb MoAbs (Immunocor, Norcross, Ga., USA) were used for the hemagglutination test to determine the Le phenotypes and for the immunoassay tests. We used the monoclonal anti-H 1E3 antibody (a generous gift from Dr. Furukawa of the Department of Legal Medicine, Gunma University School of Medicine) [14] for the immunoassay to determine H type 1 antigen. Affinity-purified goat anti-mouse IgM (p chain-specific) was obtained from Cappel, USA.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Analysis of Lea and Le^b Antigens in Human Saliva

Wang¹,

Akiyama²,

Kimura³

1994

Vox Sanguinis

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We have measured the H type 1, Le^a and Le^b antigens in the saliva from 129 Japanese individuals by a time-resolved europium ion fluorometric immunoassay using artificial antigen-albumin complexes as the reference substances. We confirmed that the amount of Le^b was larger than that of Le^a in the saliva from secretors (Le^a-b+) and vice versa in the saliva from nonsecretors (Le^a+b-). Unexpectedly, we discovered appreciable amounts of Le^b with small amounts of H type 1 in the saliva from the nonsecretors. The concentration of Le^b was about 10, 6 and 35% of the concentration of the Le^a in the saliva from the nonsecretors of the A, B and O groups, respectively. The possible formation of Le^b from Le^a in addition to the formation of Le^b from H type 1, in the salivary glands is discussed.

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Immunochemical characterization of anti-H monoclonal antibodies obtained from a mouse immunized with human saliva

Cited by 22 publications

References 18 publications

Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

Quantitative Analysis of Lea and Le^b Antigens in Human Saliva

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