Immunochemical and single molecule force spectroscopy studies of specific interaction between the laminin binding protein and the West Nile virus surface glycoprotein E domain II

Bogachek, Maria V.; Протопопова, Е. В.; Loktev, V. B.; Зайцев, Б. Н.; Favre, M.; Sekatskii, S. K.; Dietler, Giovanni

doi:10.1002/jmr.866

Cited by 16 publications

(19 citation statements)

References 42 publications

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“…We found that laminin bound to the dengue envelope protein, and several laminin binding proteins and receptors have already been suggested to act as receptors or cofactors for both dengue and West Nile virus entry in mosquito and mammalian cells (Bogachek et al, 2008; Sakoonwatanyoo, Boonsanay, and Smith, 2006). This indicates that the TAP method can accurately identify host proteins known to bind components of the virion surface by using the envelope protein.…”

Section: Discussionmentioning

confidence: 76%

Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector

et al. 2011

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West Nile and dengue viruses are (re)emerging mosquito-borne flaviviruses that cause significant morbidity and mortality in man. The identification of mosquito proteins that associate with flaviviruses may provide novel targets to inhibit infection of the vector or block transmission to humans. Here, a tandem affinity purification (TAP) assay was used to identify 18 mosquito proteins that interact with dengue and West Nile capsid, envelope, NS2A or NS2B proteins. We further analyzed the interaction of mosquito cadherin with dengue and West Nile virus envelope protein using co-immunoprecipitation and immunofluorescence. Blocking the function of select mosquito factors, including actin, myosin, PI3-kinase and myosin light chain kinase, reduced both dengue and West Nile virus infection in mosquito cells. We show that the TAP method may be used in insect cells to accurately identify flaviviral-host protein interactions. Our data also provides several targets for interrupting flavivirus infection in mosquito vectors.

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Section: Discussionmentioning

confidence: 76%

Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector

et al. 2011

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“…5). Ribosomal protein p40 [VectorBase: AAEL008083/AAEL013694] is a precursor of the high affinity laminin receptor, a putative receptor for the entry of DENV into host cells [25,47]. While mature laminin receptors are located on the cell surface, p40 has been detected in different compartments of cells, and is suggested to be a shuttle protein between the cytoplasm and the nucleus [48].…”

Section: Resultsmentioning

confidence: 99%

Response of the mosquito protein interaction network to dengue infection

Guo

Bian

et al. 2010

BMC Genomics

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BackgroundTwo fifths of the world's population is at risk from dengue. The absence of effective drugs and vaccines leaves vector control as the primary intervention tool. Understanding dengue virus (DENV) host interactions is essential for the development of novel control strategies. The availability of genome sequences for both human and mosquito host greatly facilitates genome-wide studies of DENV-host interactions.ResultsWe developed the first draft of the mosquito protein interaction network using a computational approach. The weighted network includes 4,214 Aedes aegypti proteins with 10,209 interactions, among which 3,500 proteins are connected into an interconnected scale-free network. We demonstrated the application of this network for the further annotation of mosquito proteins and dissection of pathway crosstalk. Using three datasets based on physical interaction assays, genome-wide RNA interference (RNAi) screens and microarray assays, we identified 714 putative DENV-associated mosquito proteins. An integrated analysis of these proteins in the network highlighted four regions consisting of highly interconnected proteins with closely related functions in each of replication/transcription/translation (RTT), immunity, transport and metabolism. Putative DENV-associated proteins were further selected for validation by RNAi-mediated gene silencing, and dengue viral titer in mosquito midguts was significantly reduced for five out of ten (50.0%) randomly selected genes.ConclusionsOur results indicate the presence of common host requirements for DENV in mosquitoes and humans. We discuss the significance of our findings for pharmacological intervention and genetic modification of mosquitoes for blocking dengue transmission.

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“…The same covalent attachment of proteins onto tip and sample surfaces without special linkers has been used in our previous force spectroscopy researches with WNV protein E (or its fragments) and its putative cell receptors (Bogachek et al ., , ). This similarity is very important for a direct comparison of the results obtained in these aforementioned works and in the present study.…”

Section: Methodsmentioning

confidence: 99%

Force‐induced globule–coil transition in laminin binding protein and its role for viral–cell membrane fusion

Зайцев

Benedetti

Mikhaylov

et al. 2014

J of Molecular Recognition

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The specific interactions of the pairs laminin binding protein (LBP)-purified tick-borne encephalitis viral surface protein E and certain recombinant fragments of this protein, as well as West Nile viral surface protein E and certain recombinant fragments of that protein, are studied by combined methods of single-molecule dynamic force spectroscopy (SMDFS), enzyme immunoassay and optical surface waves-based biosensor measurements. The experiments were performed at neutral pH (7.4) and acid pH (5.3) conditions. The data obtained confirm the role of LBP as a cell receptor for two typical viral species of the Flavivirus genus. A comparison of these data with similar data obtained for another cell receptor of this family, namely human αVβ3 integrin, reveals that both these receptors are very important. Studying the specific interaction between the cell receptors in question and specially prepared monoclonal antibodies against them, we could show that both interaction sites involved in the process of virus-cell interaction remain intact at pH 5.3. At the same time, for these acid conditions characteristic for an endosome during flavivirus-cell membrane fusion, SMDFS data reveal the existence of a force-induced (effective already for forces as small as 30-70 pN) sharp globule-coil transition for LBP and LBP-fragments of protein E complexes. We argue that this conformational transformation, being an analog of abrupt first-order phase transition and having similarity with the famous Rayleigh hydrodynamic instability, might be indispensable for the flavivirus-cell membrane fusion process.

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Immunochemical and single molecule force spectroscopy studies of specific interaction between the laminin binding protein and the West Nile virus surface glycoprotein E domain II

Cited by 16 publications

References 42 publications

Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector

Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector

Response of the mosquito protein interaction network to dengue infection

Force‐induced globule–coil transition in laminin binding protein and its role for viral–cell membrane fusion

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