Immunochemical and immunocytochemical study of S-100 protein in rat adipocytes

Michetti, Fabrizio; Dell'Anna, Elisabetta; Tiberio, G; Cocchia, Domenico

doi:10.1016/0006-8993(83)91032-6

Cited by 109 publications

(50 citation statements)

References 23 publications

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“…B-and T-cells were YFPϪ in all locations (data not shown). Dendritic cells have been previously reported to express S100B (Cocchia et al, 1981Michetti et al, 1983Michetti et al, , 1985. In our material, however, many YFPϩ dendritic cells were not labeled by a polyclonal antibody to S100B, suggesting that the relationship between expression of the endogenous gene and the transgene is an artifact of transgenesis.…”

Section: Resident Macrophages In Muscles and Peripheral Nerves Exprescontrasting

confidence: 57%

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Zuo¹,

Lubischer²,

Kang³

et al. 2004

J. Neurosci.

142

158

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To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.

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Section: Resident Macrophages In Muscles and Peripheral Nerves Exprescontrasting

confidence: 57%

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Zuo¹,

Lubischer²,

Kang³

et al. 2004

J. Neurosci.

142

158

View full text Add to dashboard Cite

show abstract

“…The most probable origin of S100B in peripheral blood, as previously reported in several studies, is nervous tissue, although we cannot exclude the possibility that it may also be released from other sites of concentration, such as adipose tissue (20 ). However, data on the presence of the protein in adipose tissue at the ages studied here are not conclusive.…”

contrasting

confidence: 43%

Quantitative Spectrophotometric Microplate Assay for Angiotensin-converting Enzyme in Cerebrospinal Fluid

Erickson

Cousin

et al. 2003

Clinical Chemistry

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“…URINE S100B LEVELS AND NEONATAL DEATH However, the possibility that S100B may be released, at least in part, from other sites in which it is concentrated, such as adipose tissue (29) could also be considered. Unfortunately, data on the presence of the protein in adipocytes at this stage of maturity are not available.…”

mentioning

confidence: 99%

S100B Protein in Urine of Preterm Newborns with Ominous Outcome

et al. 2005

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Prematurity is an important cause of perinatal death, and no reliable biochemical/biophysical markers exist to identify newborns with an increased mortality risk. We aimed to use S100B concentrations in urine as an early indicator of risk of neonatal death. We did a cross-sectional study using urine obtained from 165 preterm newborns, of whom 11 suffered neonatal death within the first week, 121 displayed no overt neurologic syndrome, and 33 suffered neonatal hypoxia and intraventricular hemorrhage (IVH) but not ominous outcome. Urine S100B concentrations were determined at four time-points and corrected for gestational age by conversion to multiples of median (MoM) of healthy controls of the same gestational age. Ultrasound imaging was assessed within the first 72 h from birth. In infants that died within the first week, S100B levels in urine were already higher than controls at first urination and increased progressively between the 24 and 96-h time-points. Multiple logistic regression analysis showed a significant correlation between urine S100B protein concentrations and the occurrence of neonatal death. An S100B concentration cut-off of 12.93 MoM at first urination had a sensitivity of 100% and a specificity of 97.8% for predicting an ominous outcome. The positive predictive value was 78.6%, the negative predictive value was 100%. Measurement of urine S100B protein levels in preterm newborns could be useful to identify newborns at higher risk of neonatal death. Prematurity constitutes the main cause leading to perinatal death and about 10 -15% of high-risk newborns might have an ominous outcome (1,2). The possibility of early identification of infants at higher risk of death is to date limited, inasmuch as clinical, laboratory and standard monitoring procedures may be of no avail. There is thus a constant need for practical and sensitive markers able to identify patients at higher risk as early as possible, to take immediate preventive or therapeutic measures. The inclusion of such a marker in evidence-based guidelines is eagerly awaited; however, at present no effective biochemical or biophysical tools exist to predict newborns at higher risk of perinatal death (1-4).The presence in blood of elevated concentrations of a brain constituent such as S100B protein has been described as prognostic of death in adults, and it has been suggested that measurement of this protein be included among peri-mortem procedures (5). S100B is an acidic calcium-binding protein of the EF-hand family (6) that is highly concentrated in the nervous system; it has a half-life of about 1 h and is mainly eliminated in urine (7). In this respect, raised urine S100B concentrations are a consolidated marker of brain damage in infants (8 -10) and in preterm newborns with IVH (11), whereas increased amniotic fluid S100B concentrations are a marker of fetal death (12). Because urine is a clinically accessible fluid for the measurement of S100B when monitoring newborns at high risk, we aimed to evaluate whether

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Immunochemical and immunocytochemical study of S-100 protein in rat adipocytes

Cited by 109 publications

References 23 publications

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination

Quantitative Spectrophotometric Microplate Assay for Angiotensin-converting Enzyme in Cerebrospinal Fluid

S100B Protein in Urine of Preterm Newborns with Ominous Outcome

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