Immunochemical analysis of HPV L1 capsid protein and p16 protein in liquid-based cytology samples from uterine cervical lesions

C, Yoshida; Sano, Takaaki; Kanuma, Tatsuya; Owada, N; Sakurai, Shunsuke; Fukuda, Toshio; Nakajima, Takashi

doi:10.1002/cncr.23366

Cited by 50 publications

(32 citation statements)

References 19 publications

(20 reference statements)

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“…The L1-positive/p16-positive and L1-negative/p16-positive patterns indicate that the lesion is in an early or advanced dysplastic phase, respectively. 35 Thus, the sequence of the L1/p16 expression status could change in the order of L1-negative/p16-negative, L1-positive/p16-negative, L1-positive/p16-positive, and L1-negative/p16-positive with increasing severity of the cervical lesion. 35 In our study, the L1-negative/p16-positive pattern was observed mainly in SCC and CIN-2/CIN-3 samples (Table 4), whereas the L1-positive/p16-positive pattern was observed in the minority of CIN-1 and CIN-2/CIN-3 samples.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Wang

Zhong

et al. 2010

Cancer Cytopathology

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BACKGROUND: This study was conducted to evaluate the reliability and role of cell block preparations in the diagnosis of neoplastic and preneoplastic lesions of the cervix and to improve the value of cell block preparations in diagnosing and predicting the prognosis of cervical lesions through immunostaining of p16INK4A (p16), Ki-67, and human papillomavirus (HPV) L1 capsid protein (HPV L1). METHODS: In total, 138 specimens were diagnosed on liquid-based cytology (LBC) and cell block preparations, and 63 specimens were subjected subsequently to tissue follow-up and immunostaining for p16, Ki-67, and HPV L1 on cell block sections. RESULTS: In 42 specimens that were diagnosed as low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) on cell blocks, 38 specimens (90.5%) were confirmed by histopathologic reports, and there was slightly better than 81.6% agreement between LBC and tissue follow-up. Immunointensity and cells that were positive for p16 were enhanced according to increased pathologic grade and differed statistically between cervical intraepithelial neoplasia 1 (CIN-1) and CIN-2/CIN-3 as well as SCC. The positive rates of HPV L1 decreased gradually according to the severity of cervical neoplasia, and HPV L1/p16 expression patterns were related to the severity of cervical lesions. CONCLUSIONS: The cell block preparation technique was complementary to LBC, and the authors concluded that the application of LBC combined with cell block preparations may improve the diagnostic accuracy of cytology. Immunostaining for p16 and Ki-67 on cell block preparations can help to improve the diagnostic accuracy of HSIL and SCC. A combined expression pattern of p16 and HPV L1 may serve as a valuable index for predicting prognosis and follow-up of cervical dysplastic lesions. Cancer (Cancer Cytopathol) 2010;118:47-55.

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Section: Discussionmentioning

confidence: 99%

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Wang

Zhong

et al. 2010

Cancer Cytopathology

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“…Recently, a novel technique for identifying HPV L1 capsid protein expression using immunohistologic staining has been used to predict prognostic information about early dysplastic lesions. 12,13 Yoshida et al 21 demonstrated that expression of L1 capsid protein decreased with lesion progression from LSIL to HSIL and SCC. The correlation between L1 and p16 expressions suggests that L1(−)/p16(+) has the potential for progression, whereas L1(+)/p16(−) and L1(−)/p16(−) may be nonprogressive lesions or potentially in remission.…”

Section: Discussionmentioning

confidence: 99%

Immunochemical Analysis of Human Papillomavirus L1 Capsid Protein in Liquid-Based Cytology Samples from Cervical Lesions

Xiao

Bian²,

Ma³

et al. 2010

Acta Cytologica

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“…Therefore, how to identify patients at risk of progressive cervical lesions from those infected with HPV to avoid the over treatment is a big issue in clinic. Yoshida et al [7][8][9][10][11][12] revealed that HPV L1 capsid protein and p16INK4a were prognostic biomarkers of HPV infection. In this study, we detected the expression of HPV L1 capsid protein and p16…”

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confidence: 99%

An analysis on the combination expression of HPV L1 capsid protein and p16^INK4a in cervical lesions

Huang

Nie

et al. 2010

Diagnostic Cytopathology

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Human papillomavirus (HPV) infection in cervix is the most important reason for cervical cancer, but only 2% cervical HPV infection will develop into cervical cancer. So how to identify patients at risk of progressive cervical lesions from those infected with HPV to avoid over treatment is a big issue in clinic. The aims of this study were to detect the expression of HPV L1 capsid protein and p16(INK4a) in cervical lesions and to investigate the combination expression of HPV L1 capsid protein and p16(INK4a) in cervical lesions and its diagnostic efficiency in clinic. Immunochemical method was used to detect the expression of HPV L1 capsid protein and p16(INK4a) in 169 cases of abnormal cytology. Histopathologic test was performed to identify cervical lesions of all the cases. chi(2) test and spearman's rank correlation were used for statistical analysis. The diagnostic sensitivity, specificity, positive predictive values (PPV), negative predictive values (NPV), accuracy, and the area under the receive operating characteristic (ROC) curve (denoted by A(Z)) were calculated with SPSS 13.0. All the statistical tests were two sided at the 5% level of significance. L1 expression decreased (P < 0.001), but p16(INK4a) expression increased (P < 0.001) with histopathologic diagnosis increasing. The expression rates of HPV L1 capsid protein, p16(INK4a), and L1(-)/p16(+) in cervical intraepithelial neoplasia (CIN)2, CIN3, and squamous-cell carcinoma were statistically different from those in CIN1 (P < 0.001). The expressions of HPV L1 capsid protein, L1(+)/p16(+), L1(+)/p16(-), and L1(-)/p16(-) were negatively correlated with the severity of cervical lesions (P < 0.001), whereas the expressions of p16(INK4a) and L1(-)/p16(+) were positively correlated with the severity of cervical lesions (P < 0.001). The specificity and A(Z) of combining L1 with p16 (INK4a) were statistically higher than L1 or p16 (INK4a) alone (P < 0.05). L1 and p16(INK4a) are useful biomarkers for the early diagnosis of cervical lesions. The combination of L1 and p16(INK4a) has a higher diagnostic accuracy than L1 or p16(INK4a) alone in diagnosis of cervical lesions.

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Immunochemical analysis of HPV L1 capsid protein and p16 protein in liquid-based cytology samples from uterine cervical lesions

Cited by 50 publications

References 19 publications

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Immunochemical Analysis of Human Papillomavirus L1 Capsid Protein in Liquid-Based Cytology Samples from Cervical Lesions

An analysis on the combination expression of HPV L1 capsid protein and p16^INK4a in cervical lesions

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Immunochemical analysis of HPV L1 capsid protein and p16 protein in liquid-based cytology samples from uterine cervical lesions

Cited by 50 publications

References 19 publications

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Diagnostic value of p16INK4A, Ki‐67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid‐based gynecologic cytology specimens

Immunochemical Analysis of Human Papillomavirus L1 Capsid Protein in Liquid-Based Cytology Samples from Cervical Lesions

An analysis on the combination expression of HPV L1 capsid protein and p16INK4a in cervical lesions

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An analysis on the combination expression of HPV L1 capsid protein and p16^INK4a in cervical lesions