Immunochemical activity of the Yersinia pseudotuberculosis B-antigen

Byvalov, A. A.; Dudina, L. G.; Chernyad’ev, A. V.; Konyshev, I. V.; Litvinets, Sergey G.; Ovodov, Yu. S.

doi:10.3103/s0891416815020032

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…This assumption is confi rmed by the use MAb 5-9, complementary, as shown earlier [2], to protein epitopes of Yersinia for bacteriophage receptor blockade. MAb 9 and probably MAb 8 did not infl uence phage adsorption effi ciency.…”

Section: Resultsmentioning

confidence: 93%

Immunochemical Nature of Receptors of Pseudotuberculosis Diagnostic Bacteriophage

et al. 2016

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The effect of treatment of Yersinia pseudotuberculosis cells with antibodies of various specificities on adhesiveness of pseudotuberculosis bacteriophage was analyzed by competitive inhibition technique. Bacteriophage adsorption to bacteria was sterically inhibited by monoclonal antibodies to protein epitopes of Y. pseudotuberculosis outer membrane. These results suggest that receptors of pseudotuberculosis diagnostic bacteriophage are localized on the LPS core of microbial cell.

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Section: Resultsmentioning

confidence: 93%

Immunochemical Nature of Receptors of Pseudotuberculosis Diagnostic Bacteriophage

et al. 2016

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“…The revealed phenomenon of vesicle formation in Y. pseudotuberculosis [ 126 ] has been confirmed [ 140 ], and the protein composition and some biological properties of vesicles produced by bacteria of two Y. pseudotuberculosis strains were determined. It has been shown that the vesicles produced by YPIII carry a cytotoxic necrotizing factor on their surface, inducing actin rearrangement, multinucleation, and loss of viability of HeLa cells.…”

Section: Omvs-based Vaccines Against Y Pestismentioning

confidence: 99%

“…An immunochemically detectable increase in the amount of B antigen excreted into the liquid nutrient medium is observed with a decrease in the cultivation temperature of the producer strain from 37 °C to 27 °C [ 125 ]. Only very recently, during the electron-microscopic study of B-antigen preparations, it was shown that they include large-sized formations, which, as it turned out, are OMVs [ 126 ]. Figure 2 shows a micrograph of a Y. pseudotuberculosis cell with an already budded vesicle.…”

Section: Omvs-based Vaccines Against Y Pestismentioning

confidence: 99%

“…For a more detailed immunochemical characterization of B antigen, a panel of hybridomas producing monoclonal antibodies (MAbs) raised against Y. pseudotuberculosis surface antigens, including antigen B, was generated. As it turned out, among them were MAbs to the O-side chains of lipopolysaccharide (LPS) [ 129 ], as well as MAbs against different epitopes of membrane proteins of the pathogen [ 126 ]. The first of them reacted with whole bacterial cells, B antigen and LPS preparations.…”

Section: Omvs-based Vaccines Against Y Pestismentioning

confidence: 99%

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Yersinia Outer Membrane Vesicles as Potential Vaccine Candidates in Protecting against Plague

et al. 2020

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Despite the relatively low incidence of plague, its etiological agent, Yersinia pestis, is an exceptional epidemic danger due to the high infectivity and mortality of this infectious disease. Reports on the isolation of drug-resistant Y. pestis strains indicate the advisability of using asymmetric responses, such as phage therapy and vaccine prophylaxis in the fight against this problem. The current relatively effective live plague vaccine is not approved for use in most countries because of its ability to cause heavy local and system reactions and even a generalized infectious process in people with a repressed immune status or metabolic disorders, as well as lethal infection in some species of nonhuman primates. Therefore, developing alternative vaccines is of high priority and importance. However, until now, work on the development of plague vaccines has mainly focused on screening for the potential immunogens. Several investigators have identified the protective potency of bacterial outer membrane vesicles (OMVs) as a promising basis for bacterial vaccine candidates. This review is aimed at presenting these candidates of plague vaccine and the results of their analysis in animal models.

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Force interactions between Yersiniae lipopolysaccharides and monoclonal antibodies: An optical tweezers study

Konyshev

Byvalov

Ананченко

et al. 2020

Journal of Biomechanics

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Immunochemical activity of the Yersinia pseudotuberculosis B-antigen

Cited by 5 publications

References 12 publications

Immunochemical Nature of Receptors of Pseudotuberculosis Diagnostic Bacteriophage

Immunochemical Nature of Receptors of Pseudotuberculosis Diagnostic Bacteriophage

Yersinia Outer Membrane Vesicles as Potential Vaccine Candidates in Protecting against Plague

Force interactions between Yersiniae lipopolysaccharides and monoclonal antibodies: An optical tweezers study

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