Immunocharacterization of the DNA puff BhC4-1 protein of Bradysia hygida (Diptera: Sciaridae)

Monesi, Nádia; Silva, J.A; Martins, P.C.M.; Teixeira, Ana Beatriz Vilela; Dornelas, E.C; Moreira, Jorge E.; Larson, Maria Luisa Paçó

doi:10.1016/j.ibmb.2004.02.010

Cited by 13 publications

(21 citation statements)

References 38 publications

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“…The BhC4-1 protein is expressed at the time DNA puff C4 is formed and is found both in secretory granules, in the salivary gland, and in fibrous structures present in the saliva of fourth instar larvae (Monesi et al, 2004), a subcellular localization consistent with the proposed role for DNA puff proteins. It is noteworthy that in spite of the availability of complete genome sequences for different insect species, the only identified proteins that present similarity to the BhC4-1 protein are the products of the C8 and B2 DNA puffs of Rhynchosciara americana (Frydman et al, 1993;Penalva et al, 1997;Santelli et al, 2004), strongly suggesting that the BhC4-1 protein is a member of a protein family unique to sciarids.…”

mentioning

confidence: 56%

“…The biological purpose of gene amplification in Sciaridae appears to be the production of large quantities of the proteins that are employed to build the cocoon in which the larvae pupate (de-Almeida, 1997;Fontes et al, 1999;Monesi et al, 2004;Winter et al, 1977a,b). The BhC4-1 protein is expressed at the time DNA puff C4 is formed and is found both in secretory granules, in the salivary gland, and in fibrous structures present in the saliva of fourth instar larvae (Monesi et al, 2004), a subcellular localization consistent with the proposed role for DNA puff proteins.…”

mentioning

confidence: 99%

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Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Lecci

Malta

Flausino

et al. 2008

Genesis

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Summary: The sciarid DNA puff C4 BhC4-1 gene is amplified and transcribed in salivary glands at the end of the larval stage. In transgenic Drosophila, the BhC4-1 promoter drives transcription in prepupal salivary glands and in the ring gland of late embryos. A bioinformatics analysis has identified 162 sequences similar to distinct regions of the BhC4-1 proximal promoter, which are predominantly located either in 5 0 or 3 0 regions or introns in the Drosophila melanogaster genome. A significant number of the identified sequences are found in the regulatory regions of Drosophila genes that are expressed in the salivary gland. Functional assays in Drosophila reveal that the BhC4-1 proximal promoter contains both a 129 bp (2186/258) salivary gland enhancer and a 67 bp

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mentioning

confidence: 56%

mentioning

confidence: 99%

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Lecci

Malta

Flausino

et al. 2008

Genesis

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“…The BhC4-1 protein is a 43 kDa polypeptide secreted by the salivary gland at the time DNA puff C4 is formed. Immunoelectron microscopy experiments revealed the presence of the BhC4-1 protein both in salivary gland secretory granules and in fibrous structures present in the saliva (Monesi et al, 2004). The BhC4-1 protein is similar to the products of the C8 and B2 DNA puffs of the sciarid Rhynchosciara americana (Frydman et al, 1993;Penalva et al, 1997;Santelli et al, 2004).…”

Section: Introductionmentioning

confidence: 92%

Broad‐Complex, E74, and E75 early genes control DNA puff BhC4‐1 expression in prepupal salivary glands

Basso

Neves

Monesi

et al. 2006

Genesis

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The DNA puff BhC4-1 gene of the sciarid Bradysia hygida is induced in salivary glands prior to the pupal molt as a secondary response to the increase in ecdysone titers. Previous studies demonstrated that the BhC4-1 promoter is activated in transgenic Drosophila melanogaster salivary glands as a late response to the ecdysone peak that triggers metamorphosis, revealing that this aspect of BhC4-1 transcriptional regulation is conserved in the Drosophila background. To identify regulators of BhC4-1 expression, we utilized a candidate gene approach and tested the roles of the ecdysone-induced genes BR-C, E74, and E75. Our results reveal that the BR-C Z3 isoform is essential for BhC4-1-lacZ induction in prepupal salivary glands and constitute the first demonstration of the participation of early genes products on DNA puff genes regulation.

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“…The biological purpose of DNA amplification appears to be the production of abundant DNA templates that enables the synthesis of large amounts of protein during a short period of time (de Almeida, 1997;Laicine et al, 1984;Winter et al, 1977a,b). Accordingly, DNA puff proteins are expressed in the salivary glands at the same time as DNA puffs are formed and are present in the salivary gland secretion used by the larvae to build the cocoon (Fontes et al, 1999;Monesi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Amplification and expression of a salivary gland DNA puff gene in the prothoracic gland of Bradysia hygida (Diptera: Sciaridae)

Candido-Silva

Machado

Hartfelder

et al. 2015

Journal of Insect Physiology

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Immunocharacterization of the DNA puff BhC4-1 protein of Bradysia hygida (Diptera: Sciaridae)

Cited by 13 publications

References 38 publications

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Broad‐Complex, E74, and E75 early genes control DNA puff BhC4‐1 expression in prepupal salivary glands

Amplification and expression of a salivary gland DNA puff gene in the prothoracic gland of Bradysia hygida (Diptera: Sciaridae)

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