Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups

Preparation of morphology-controllable PGMA-DVB microspheres by introducing Span 80 into seed emulsion polymerization

“…For instance, the introduction of Fe 3 O 4 nanoparticles, uorescent molecules, catalyst or proteins may help us to obtain a series of different functional hybrid microspheres. 42,[47][48][49] When the amount of Span 80 was reached to 0.9 mL, the morphology of particles changed dramatically. As show in Fig.…”

PES magnetic microspheres: preparation and performance for the removal of endocrine disruptor-BPA

“…In this way they can be separated directly from the environment. Magnetic microspheres have attracted much attention in recent years and will be potentially used in many elds, such as wastewater treatment, 19,20 enzyme immobilization, 21 magnetic resonance imaging (MRI) 22 and cell separation, 23 etc. Conventionally, the preparation methods include monomer polymerization 24,25 and embedding method, 26,27 both involving the synthesis of magnetic particles as well as the microspheres and the following binding of particles and microspheres.…”

Oriented immobilization of antibody through carbodiimide reaction and controlling electric field

“…Ab is a special class of glycoproteins containing approximately 3 % carbohydrate located on Fc region [10]. Carbohydrate can be oxidized by sodium periodate to form aldehyde groups, and Ab is consequently bound to amino group of support surface via the Schiff-base reaction [11][12][13]. Oxidation method was a convenient and effective oriented immobilization technique, while it may result in excessive damage to Ab reactivity [2].…”