ImmunoCAP and HY•TEC enzyme immunoassays in the detection of allergen‐specific IgE compared with serial skin end‐point titration by receiver operating characteristic analysis

Abstract

The 2 in vitro testing systems are comparable for most of the allergens, except for A fumigatus, English plantain, lamb's-quarter, and white ash tree; ImmunoCAP and HY*TEC would benefit from improved technology for detecting these specific IgE antibodies. The HY*TEC assay did not give acceptable results for A fumigatus. The overall assay characteristics were good to excellent for each system tested.

Use of a chimeric ELISA to investigate immunoglobulin E antibody responses to Der p 1 and Der p 2 in mite‐allergic patients with asthma, wheezing and/or rhinitis

“…In addition, some medications can suppress positivity of skin tests [11]. Alternatively, allergen‐specific serum IgE can be measured by in vitro methods [12–19], including the classic radioallergosorbent test (RAST) originally described in 1967 [20], which requires utilization of radioactive reagents. Results of methods previously used for assaying IgE antibodies, including RAST, were expressed in arbitrary units and did not accurately determine the levels of IgE antibody.…”

Oral Challenges with Four Apple Cultivars Result in Significant Differences in Oral Allergy Symptoms

“…At the time of testing, all subjects were free from allergic symptoms and healthy with no ongoing upper respiratory tract -disease or other treatments that could possible interfere with allergic reactions. The number of circulating IgE antibodies to apple and specific birch allergens was measured in the blood of 11 individuals with ImmunoCAP which is a Fluor immunoassay (Phadia AB, Uppsala, Sweden) [16]. All assays were performed at the Department of Immunology, University Hospital Skane.…”