Immunoblot analysis of the humoral immune response to cytomegalovirus in non-pregnant and pregnant guinea pigs

Bu, Fang; Griffith, Brigitte P.

doi:10.1007/bf01311292

Cited by 2 publications

(1 citation statement)

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“…The ELISA titer was defined as the reciprocal of the highest dilution that produced an absorbance >0.10 and was twice the absorbance observed against the control antigen. Western blots were performed on sera as described elsewhere [30]. …”

Section: Methodsmentioning

confidence: 99%

Intravaginal cytomegalovirus (CMV) challenge elicits maternal viremia and results in congenital transmission in a guinea pig model

Olejniczak

Choi

McVoy

et al. 2011

Virol J

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BackgroundThe objective of this study was to compare intravaginal (ivg) and subcutaneous (sc) administration of the guinea pig cytomegalovirus (GPCMV) in pregnant and non-pregnant guinea pigs. These studies tested the hypotheses that ivg infection would elicit immune responses, produce maternal viremia, and lead to vertical transmission, with an efficiency similar to the traditionally employed sc route.ResultsFour groups of age- and size-matched guinea pigs were studied. Two groups were pregnant, and two groups were not pregnant. Animals received 5x105 plaque-forming units (PFU) of a GPCMV reconstituted from an infectious bacterial artificial chromosome (BAC) construct containing the full-length GPCMV genome. Seroconversion was compared by IgG ELISA, and viremia (DNAemia) was monitored by PCR. In both pregnant and non-pregnant animals, sc inoculation resulted in significantly higher serum ELISA titers than ivg inoculation at 8 and 12 weeks post-infection. Patterns of viremia (DNAemia) were similar in animals inoculated by either sc or ivg route. However, in pregnant guinea pigs, animals inoculated by both routes experienced an earlier onset of DNAemia than did non-pregnant animals. Neither the percentage of dead pups nor the percentage of GPCMV positive placentas differed by inoculation route.ConclusionsIn the guinea pig model of congenital CMV infection, the ivg route is as efficient at causing congenital infection as the conventional but non-physiologic sc route. This finding could facilitate future experimental evaluation of vaccines and antiviral interventions in this highly relevant animal model.

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Section: Methodsmentioning

confidence: 99%