Immunoassays in Therapeutic Drug Monitoring

Blecka, Lawrence J.; Jackson, Gloria

doi:10.1016/s0272-2712(18)30749-2

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…When a "free" tracer compound (i.e., unbound to an antibody) is excited by linearly polarized light, its rotation is much faster than the corresponding tracer-antibody conjugate and the molecules are more randomly oriented; therefore, the emitted light is depolarized. Thus, fluorescent polarization provides a quantitative means for measuring the amount of tracerantibody conjugate produced in a competitive binding immunoassay (5).…”

Section: Introductionmentioning

confidence: 99%

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Immunoassay Reagents for Thyroid Testing. 1. Synthesis of Thyroxine Conjugates

Adamczyk

Fino²,

Fishpaugh³

et al. 1994

Bioconjugate Chem.

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Section: Introductionmentioning

confidence: 99%

“…N-Acetyl-L-thyroxine (5) (1.0 g, 1.2 mmol) was dissolved in tetrahydrofuran (50 mL). iV-Hydroxysuccinimide (170 mg, 1.5 mmol) and 1,3-dicyclohexylcarbodiimide (300 mg, 1.5 mmol) were added and the reaction stirred under nitrogen for 3 days.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay Reagents for Thyroid Testing. 1. Synthesis of Thyroxine Conjugates

Adamczyk

Fino²,

Fishpaugh³

et al. 1994

Bioconjugate Chem.

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“…Traditional methods for determining drug concentrations are based on automated immunoassays [31] or liquid chromatography. Immunoassays can be affected by matrix interferences and lack of specificity [31].…”

Section: Discussionmentioning

confidence: 99%

24/7 Therapeutic Drug Monitoring of Beta-Lactam Antibiotics with CLAM-2000

Khromov,

Dihazi,

Brockmeyer

et al. 2023

Antibiotics

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Background: The aim of this study was to evaluate the CLAM-2000 automated preanalytical sample preparation module with integrated liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS) as a method for 24/7 therapeutic drug monitoring (TDM) of beta-lactam antibiotics in routine clinical diagnostics. Methods: Method validation was performed using quality control samples. Method comparison was performed with routine samples from patients treated with beta-lactam antibiotics. Results: The determination of piperacillin, meropenem, ceftazidime, flucloxacillin, and cefotaxime was performed using D5-piperacillin and D6-meropenem as internal standards. The linearity of the method was within the therapeutic range of beta-lactam antibiotics. The imprecision and accuracy data obtained from quality control samples were within 15%, and the imprecision of patient samples on the instrument was less than the 5% coefficient of variation (CV). Internal standards stored in the instrument at 9 °C for at least one week were stable, which facilitated reagent use and storage. Conclusion: The CLAM-2000 (Shimadzu, Kyoto, Japan) provides reproducible results as an established routine instrument and is a useful tool for 24/7 TDM of beta-lactam antibiotics in routine clinical diagnostics.

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“…Therapeutic drug monitoring has been performed using immunoassays for a long time [15][16][17][18][19][20]. However, the selectivity of immunoassays is limited.…”

Section: Introductionmentioning

confidence: 99%

LC–MS3 Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring

Cao

et al. 2022

Molecules

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This study developed a detection method based on the strategy of HPLC/MS3 and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS3) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP® 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D2N15 were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 μL of sample plasma could obtain the result of LLOD 0.5 µg/mL. The intra-day and inter-day precision was <8.23%, and accuracy was between −1.74% and 2.92%. This method was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment.

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Immunoassays in Therapeutic Drug Monitoring

Cited by 8 publications

References 21 publications

Immunoassay Reagents for Thyroid Testing. 1. Synthesis of Thyroxine Conjugates

Immunoassay Reagents for Thyroid Testing. 1. Synthesis of Thyroxine Conjugates

24/7 Therapeutic Drug Monitoring of Beta-Lactam Antibiotics with CLAM-2000

LC–MS3 Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring

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