Immunoassays for trifloxystrobin analysis. Part II. Assay development and application to residue determination in food

Mercader, Josep V.; López-Moreno, Rosario; Esteve‐Turrillas, Francesc A.; Abad‐Somovilla, Antonio; Abad‐Fuentes, Antonio

doi:10.1016/j.foodchem.2014.04.053

Cited by 10 publications

(6 citation statements)

References 19 publications

(2 reference statements)

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“…According to the determined results for spiked orange samples, the recovery rates of the new ELISA assay were 82.73-104.35% for kresoxim-methyl and 91.1-92.43% for trifloxystrobin, which were the values consistent with those of the above spectrometry examination. In Mercader's study, the ELISA method was developed for the detection of trifloxystrobin and kresoxim-methyl, and the results were obtained for strawberry, tomato, and cucumber samples spiked with trifloxystrobin, which showed the limit of detection below 0.1-0.3 ng/ml and better than in our study, and excellent recovery was at 0.01 g/kg (Mercader et al 2008(Mercader et al , 2014b. The difference in detection limit could come from different antibodies in the two above-mentioned ELISA assays.…”

Section: Discussionsupporting

confidence: 47%

“…In Europe and in the USA, MRLs for kresoxim-methyl range between 0.05 and 1.5 mg/kg Czech J. Food Sci., 34, 2016(5): 429-438 doi: 10.17221/275/2015 in most fruits and vegetables (Mercader et al 2014a), and for trifloxystrobin they range from 0.05 mg/kg to 30 mg/kg in authorised crops (Mercader et al 2014b). The most stringent limits of trifloxystrobin and kresoxim-methyl concentrations in oranges are 0.02-2.0 and 0.05 mg/kg, respectively, in the European Union, and 0.05-10.0 and 0.03 mg/kg in Japan (Campillo et al 2010).…”

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

“…Enzyme-linked immunosorbent assay (ELISA) was the preferred assay for monitoring pesticide active ingredients and metabolites among the immunoassay formats. Mercader developed the ELISA methods for the detection of trifloxystrobin and kresoxim-methyl, and the results showed that the limit of detection was 0.3 and 0.1 ng/ml for kresoxim-methyl and trifloxystrobin, respectively (Mercader et al 2008(Mercader et al , 2014b. However, Mercader's method could not simultaneously detect two strobilurin fungicides.…”

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

See 2 more Smart Citations

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Yang¹,

Zhu²,

Liu³

et al. 2016

Czech J. Food Sci.

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Yang W., Zhu J., Liu M.Q., Dai X.J. (2016): Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges. Czech J. Food Sci., 34: 429-438.To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active group, (E)-2-(2-bromophenyl)-2-(methoxyimino) acetic acid methyl ester (OEBr) in kresoxim-methyl and trifloxystrobin. The immunogen and coating antigen were respectively prepared through conjugating the above-mentioned hapten with BSA and OVA by the mixed anhydride and activated ester methods, and polyclonal antibodies were produced by immunised rabbits. An enzyme-linked immunosorbent assay was developed for simultaneous detection of kresoxim-methyl and trifloxystrobin. In ELISA, the antiserum showed high affinity and sensitivity to kresoxim-methyl and trifloxystrobin, and their IC 50 value and detection limit (expressed as IC 10 ) were 14.7 and 0.0044 µg/ml, respectively, for kresoxim-methyl, and 22.9 and 0.014 µg/ml, respectively, for trifloxystrobin. The cross-reaction rate was below 0.1% for other strobilurin fungicides. Recovery study of ELISA from spiked samples of homogenised peeled oranges (final concentrations of 100, 10, and 1 µg/ml) resulted in recovery levels in the range of 82-104%.

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Section: Discussionsupporting

confidence: 47%

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Yang¹,

Zhu²,

Liu³

et al. 2016

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…Conventional techniques such as gas chromatography [3], high-performance liquid chromatography [4], thin-layer chromatography [5], supercritical fluid chromatography [6], gas and liquid chromatography combined with mass spectrometry [5,7], capillary electrophoresis [8,9], enzyme inhibition method [5,10], immunoassay method [5,11], and bio-sensor method [5,12], are also used to measure the concentration of pesticide residue in agricultural products.…”

Section: Introductionmentioning

confidence: 99%

Developing a Vis/NIR spectroscopic system for fast and non-destructive pesticide residue monitoring in agricultural product

Jamshidi

Mohajerani

Jamshidi³

2016

Measurement

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In this research, an optical system based on fibre optic Vis/NIR spectroscopy combined with chemometrics methods and software as a graphical user interface (GUI) was developed and presented for fast and non-destructive detection and determination of pesticide residues in agricultural products (a case study on diazinon in intact cucumbers). Vis/NIR spectra of cucumber samples without and with different concentrations of diazinon residue were analyzed at the range of 450-1000 nm. Partial least squares (PLS) regression models were developed based on chemical reference measurements and the spectral information of the samples after performing different pre-processing methods. Moreover, partial least squares-discriminant analysis (PLS-DA) models were developed based on different spectral pre-processing techniques to classify cucumbers with contents of diazinon below and above the maximum residue limits (MRL) as safe and unsafe samples, respectively. Finally, user-friendly software as a GUI was created based on the best PLS and PLS-DA models developed for prediction of diazinon contents in the samples and for classification of intact cucumbers by the absence/presence of diazinon residues, respectively. Evaluation of the system and software designed based on the best developed PLS and PLS-DA models indicated good performance for measuring and detection of

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“…In addition, gas chromatography (GC) (Jyot et al, 2010;Kaur et al, 2012;Mohapatra, 2014;Sahoo et al, 2012), liquid chromatography with tandem mass spectrometry (LC-MS/MS) (Chen et al, 2014), ASE-LC-MS/MS (Chen et al, 2008) and immunochemical methods (Mercader et al, 2014) have been developed to analyze trifloxystrobin in foodstuffs. Nevertheless, few methodologies were available for rapid analysis of trifloxystrobin in environmental samples (Chen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Track of fate and primary metabolism of trifloxystrobin in rice paddy ecosystem

Cao

Wang

et al. 2015

Science of The Total Environment

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Immunoassays for trifloxystrobin analysis. Part II. Assay development and application to residue determination in food

Cited by 10 publications

References 19 publications

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Developing a Vis/NIR spectroscopic system for fast and non-destructive pesticide residue monitoring in agricultural product

Track of fate and primary metabolism of trifloxystrobin in rice paddy ecosystem

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