Immunoassay of Parathyroid Hormone by Quantitative Complement Fixation12

Tashjian, Armen H.; Levine, Lawrence; Munson, Paul L.

doi:10.1210/endo-74-2-244

Cited by 38 publications

(30 citation statements)

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“…(range 0-08-0-24). Compared with other CF assay procedures (Tashjian et al 1964;Brody, 1966), the precision of the present method appears to be nearly the same as that reported by Tashjian et al (1964).…”

Section: Discussionsupporting

confidence: 78%

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Immunoassay of Follicle-Stimulating Hormone in Human Urine and Serum by Quantitative Complement Fixation

Mori¹

1968

Journal of Endocrinology

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An immunoassay method for follicle-stimulating hormone (FSH) in human urine and serum by means of complement fixation is described in detail. The assay detects 0\m=.\6\p=n-\1\m=.\5m-u. FSH, and does not require any modification of antigens. Good agreement is shown between the biological and immunological estimations of FSH activity of urinary and serum extracts. Immunological estimations of the urinary FSH excretion in 15 normal men and 24 normal postmenopausal women gave the mean values of 8\m=.\5 (range 2\ m=. \ 6\ p=n-\ 15\m=.\4) i.u. and 116\m=.\7 (range 30\m=.\1\p=n-\213\m=.\3)i.u./24 hr., respectively. The mean serum FSH levels determined immunologically in ten normal men and 14 normal postmenopausal women were 6\m=.\2 (range 3\ m=. \ 3\ p=n-\ 13\ m=. \ 9) m-u. and 58\m=.\1 (range 39\ m=. \ 7\ p=n-\ 94\ m=. \ 0)m-u./ml., respectively. The pattern of daily urinary FSH excretion during the menstrual cycle of a normal woman showed an insignificant rise during the follicular phase and a peak immediately after the thermal nadir.

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Section: Discussionsupporting

confidence: 78%

“…Preliminary aspects of an immunoassay for FSH by CF have been described by Butt (1967). The same CF technique has been employed for immunoassays of human chorionic gonadotrophin (Brody & Carlström, 1961;Brody, 1966) and of parathyroid hormone (Tashjian, Levine & Munson, 1964).…”

Section: Introductionmentioning

confidence: 99%

Immunoassay of Follicle-Stimulating Hormone in Human Urine and Serum by Quantitative Complement Fixation

Mori¹

1968

Journal of Endocrinology

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“…The hormone is anti genic, and sensitive immunoassays have been developed based on comple ment fixation (126) or radioimmunoassay (127). The intravenous infusion of antibodies to bovine PTH caused hypocalcemia in rats and rabbits but not in guinea pigs (128), The response in the first two species paralleled that following parathyroidectomy and was presumably due to neutralization of endogenous hormone.…”

Section: Parathyroid Hormonementioning

confidence: 99%

Calcitonin and Parathyroid Hormone

Copp¹

1969

Annu. Rev. Pharmacol.

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“…The radioimmunoassay for PTH (89,90) is proving to be a useful tool in physiologic studies concerned with secretion of the hormone and circulating concentrations in blood (91,92). One of the major difficulties in obtaining a workable immunoassay for PTH appears to be in the production of high titer antisera.…”

Section: Assaymentioning

confidence: 99%