Immunoassay of Carcinogen Modified DNA

Strickland, Paul T.; Boyle, John M.

doi:10.1016/s0079-6603(08)60374-8

Cited by 57 publications

(13 citation statements)

References 168 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunochemical methods can be used to quantitate DNA damage if alternative assay methods are available for the damage of interest. This approach has been extensively used to quantitate base damage produced in DNA by chemical carcinogens (for reviews see (51,52)) and more recently for irradiated DNA (for review see (53)). …”

Section: Resultsmentioning

confidence: 99%

Immunochemical Quantitation of Thymine Glycol in Oxidized and X-Irradiated DNA

Hubbard¹,

Huang²,

Laspia³

et al. 1989

Radiation Research

View full text Add to dashboard Cite

The present study demonstrates the usefulness of immunochemical assays for quantitating modified bases in oxidized and X-irradiated DNA. Escherichia coli, phi X174 RF I, PM2, and M13 DNA containing thymine glycols introduced by OsO4 oxidation were used as antigens in a direct enzyme-linked immunosorbent assay (ELISA). The number of thymine glycols per DNA molecule was determined by reactivity with antithymine glycol antibody standardized either to the acetol fragment assay or to the number of Escherichia coli endonuclease III-sensitive sites. The number of thymine glycols was also determined in phi X174 RF I DNA X-irradiated in either phosphate or Tris buffer under air. Using a direct ELISA with phi X174 RF I DNA irradiated in a phosphate buffer solution, the anti-thymine glycol antibody detected damage at the level of 40 Gy. The immunochemical assay was sensitive, specific, quantitative, and independent of DNA structure.

show abstract

Section: Resultsmentioning

confidence: 99%

Immunochemical Quantitation of Thymine Glycol in Oxidized and X-Irradiated DNA

Hubbard¹,

Huang²,

Laspia³

et al. 1989

Radiation Research

View full text Add to dashboard Cite

show abstract

“…The two most frequently employed DNA adduct methodologies, immunoassays and 32P-postlabeling, will be discussed briefly. Antisera elicited against DNA adducts and carcinogen-modified DNA samples (17)(18)(19) have been widely utilized to quantify and localize xenobiotic-induced DNA damage (20)(21)(22)(23) and to measure DNA adduct formation in human tissues (24,25 (70,71) and PAHs (72,73), while GC-MS is routinely applied for 4-aminobiphenyl (74), N-nitrosamines (12,(75)(76)(77)(78), and tobacco-specific nitrosamines (79). Atomic absorbance spectrometry is used for cisplatin (80,81) and electrochemical detection is used for oxidative DNA damage (82,83 dosimetry of aflatoxin exposure, 42 individuals in the Guanxi region of China (89) were studied.…”

Section: Methodological Approaches To Human Dna Adduct Monitoringmentioning

confidence: 99%

DNA adducts as exposure biomarkers and indicators of cancer risk.

Poirier

1997

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…Tetrahexylammonium hydrogen sulphate (THAHSO,) (purity > 98%) was from Fluka (Buchs, Switzerland). Heptachlor (1,4,5,6,7,8,8-heptachloro-3~,4,7,7a-tetrahydr0-4,7-methano-1 H-indene) (99 %) was obtained from Lab. Dr S. Ehrenstorfer (Augsburg, Germany).…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

Electrophoric labelling of nucleosides for sensitive analysis by negative ion chemical ionization gas chromatography/mass spectrometry

Teixeira

Werken

Stavenuiter

et al. 1992

Biol. Mass Spectrom.

View full text Add to dashboard Cite

The formation and analytical utility of several electrophoric derivatives of native and oxidatively modified nucleosides of deoxythymidine and deoxyguanosine were studied. Derivatives include the incorporation of trimethylsilyl, acetyl or perfluoroacyl groups together with pentafluorobenzyl. Acetylation followed by pentafluorobenzylation was the mmt favourable method. In general, the combined information from the electron impact, positive ion chemical ionization and negative ion chemical ionization spectra was well suited for structure elucidation purposes. The acetyl-pentafluorobenzyl derivative of deoxythymidine was prepared in quantitative yield and was very sensitive on analysis by gas chromatography negative ion chemical ionization. Injection of 0.45 fmol of Y,S-bis(O-acetyl)-S pentafluorobenzyldeoxythymidine, in multiple ion detection experiments, gave a response with a signal-to-noise ratio larger than 10.

show abstract

Immunoassay of Carcinogen Modified DNA

Cited by 57 publications

References 168 publications

Immunochemical Quantitation of Thymine Glycol in Oxidized and X-Irradiated DNA

Immunochemical Quantitation of Thymine Glycol in Oxidized and X-Irradiated DNA

DNA adducts as exposure biomarkers and indicators of cancer risk.

Electrophoric labelling of nucleosides for sensitive analysis by negative ion chemical ionization gas chromatography/mass spectrometry

Contact Info

Product

Resources

About