Immunoassay for the Quantitation of Low Molecular Weight Compounds in Plants

Weiler, Elmar W.

doi:10.1007/978-3-642-74846-2_2

Cited by 8 publications

(2 citation statements)

References 356 publications

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“…1). It is a common observation (for examples, see Weiler, 1990) that exposed hapten structures are dominant epitopes selecting for higher-affinity antibody paratopes. This was also observed in the case of the MAB descnbed For the final experiments, leaves of P. vulgaris were either worked up directly or were allowed to lose 10-15 % of their water at ambient temperature after excision from the plant.…”

Section: Discussionmentioning

confidence: 99%

The Determination of Phaseic Acid by Monoclonal Antibody‐Based Enzyme immunoassay

et al. 1993

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A monoclonal antibody PA3‐2‐B3, IgG1 (Λ) is described which specifically recognizes phaseic acid and shows very little cross‐reactivity (0.14%) with abscisic acid or dihydrophaseic acid (0.88%). Based on this antibody, an enzyme immunoassay was developed which displays a linearity range from 15 pg to 3 ng of phaseic acid. Results obtained with this assay agree with those obtained by gas chromatography‐electron capture detection. Using the novel enzyme immunoassay, as well as an established immunoassay for abscisic acid, levels of these two compounds in leaves of Phaseolus vulgaris were determined as a function of plant age, water stress, recovery from stress, and feeding of abscisic acid through the transpiration stream. The production of phaseic acid in a microsomal system from bean leaves was demonstrated. The results show a regulation of the plant's capacity to metabolize abscisic acid to phaseic acid as a function of water stress.

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Section: Discussionmentioning

confidence: 99%

The Determination of Phaseic Acid by Monoclonal Antibody‐Based Enzyme immunoassay

et al. 1993

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“…For the determination of polychlorinated biphenyls, a DAIA was shown, using a catalytic dechlorination to obtain a single product (biphenyl), which was detected by immunoassay (36). In other immunoassays (e.g., for indole-3-acetic acid) the methylation by diazomethane of the analyte was used to improve selectivity and sensitivity of the analytical procedure (37,38). In this paper, the generation of antibodies to NAG and their characterization are described.…”

Section: Introductionmentioning

confidence: 99%

Development of Antibodies for the Detection of N-Acetyl-glufosinate

Weller

Diemer

Wersching

et al. 2003

J. Agric. Food Chem.

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Glufosinate is a widely used herbicide, which is difficult to detect by conventional analytical techniques. For many other herbicides, suitable antibodies have been raised for immunoassay development. Unfortunately, glufosinate is a very small molecule and difficult to immunize with. Thus, a derivatization-assisted immunoassay (DAIA) using the target analyte N-acetyl-glufosinate (NAG) was constructed. The activated hapten was synthesized by a new approach, using a homobifunctional cross-linker suberic acid bis(N-hydroxysuccinimide ester). The preparation of a suitable conjugate, the immunization, and the characterization of polyclonal antibodies are shown. The determination of the conjugation density (hapten density) of the immunogens was performed by four different methods (high-performance liquid chromatography with a refractive index detector, total reflection X-ray fluorescence, inductively coupled plasma mass spectrometry, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry), which gave similar results. The limit of detection was 17 mug/L NAG in water for the direct competitive enzyme immunoassay. NAG is also a main metabolite of glufosinate in resistant transgenic plants. The antibodies might be useful for the selective detection of NAG in the presence of the parent compound glufosinate (cross-reactivity 0.13%) and other metabolites.

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Development of a one-step enzyme immunoassay for the quantitation of gibberellin A3 in barley seeds

Bounaix

Doumas²

1995

Plant Growth Regul

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Immunoassay for the Quantitation of Low Molecular Weight Compounds in Plants

Cited by 8 publications

References 356 publications

The Determination of Phaseic Acid by Monoclonal Antibody‐Based Enzyme immunoassay

The Determination of Phaseic Acid by Monoclonal Antibody‐Based Enzyme immunoassay

Development of Antibodies for the Detection of N-Acetyl-glufosinate

Development of a one-step enzyme immunoassay for the quantitation of gibberellin A3 in barley seeds

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