Immunoassay for the detection and quantitation of infectious human retrovirus, lymphadenopathy-associated virus (LAV)

Definition of the range and distribution of human immunodeficiency virus macrophage tropism using PCR-based infectivity measurements

“…PBL infectivity assays were read after 6 to 9 days with any strain of HIV, whereas ELISA requires a 9 day incubation even with the most rapidly replicating laboratory isolates (McDougal et al, 1985). In assays performed with m~b, PCR produced comparatively stronger signals than ELISA, probably due to the low release of HIV virions by these cells Orenstein et al, 1988), and also to the tendency of HIV to accumulate unintegrated viral DNA in high copy number (Shaw et al, 1984;Pang et al, 1990).…”

Thermal inactivation of the acquired immunodeficiency syndrome virus, human T lymphotropic virus-III/lymphadenopathy-associated virus, with special reference to antihemophilic factor.

“…The microculture assay for the titration of infectious HTLV-HII/ LAV has been described in detail (3). Briefly, PHA-stimulated normal human lymphocytes are inoculated with serial 10-fold dilutions of virus inoculum and incubated for 18 h at 370C.…”

“…The supernates are then assayed for virus by an antigen capture assay (3). Infectious virus titer (ID-50) is defined as the reciprocal of the dilution at which 50% of the cultures are positive for virus (3,4). Previously established (3) features of the infectivity assay system relevant to the interpretation of the thermal inactivation experiments include the following: tht antigen capture assay is as sensitive as the RT assay for detecting supernate virus (3).…”

“…Previously established (3) features of the infectivity assay system relevant to the interpretation of the thermal inactivation experiments include the following: tht antigen capture assay is as sensitive as the RT assay for detecting supernate virus (3). The ID-50 assay, in turn, is several orders of magnitude more sensitive than either assay for detecting small amounts of virus (3). In the ID-O50 assay, active virus replication is required to obtain a measurable titer.…”

The tragic history of AIDS in the hemophilia population, 1982–1984

“…Dr Steve McDougal in my division at the CDC's immunology laboratory developed an assay for quantifying the virus in various samples in spring 1984 [32]. The author discussed with Dr McDougal the urgent need to determine whether the virus was heat sensitive.…”