Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors

A novel ratiometric fluorescence strategy is developed for specific detection of folic acid (FA) by using 11-mercaptoundecanoic acid protected gold nanoclusters (AuNCs@MUA). In this design, the fluorescence color of the probe can be switched among red, pink, violet and blue by varying the concentration of FA.AuNCs@MUA possesses strong fluorescence peaking at 612 nm (R-signal) and FA exhibits blue emissive auto-fluorescence at 446 nm (B-signal), showing a large emission shift of $170 nm. When AuNCs@MUA approaches FA through electrostatic binding, the R-signal decreases while the B-signal increases with titration of FA. Based on the above phenomenon, a radiometric analysis platform is constructed for FA target detection, with a wide linear response range from 0 to 20 mM, and an excellent detection limit of 26 nM. This new ratiometric strategy exhibits low background, and wide signal changes in a low concentration range, which presents obvious advantages over most previous FA detections based on single-responsive fluorescence methods. Furthermore, the proposed method is successfully applied to determine FA in human serum samples.

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“…As-synthesized AuNCs@MUA in this work are prepared according to one-pot reduction method by using NaBH 4 . The TEM image in Fig.…”

Section: Synthesis and Characterization Of Auncs@muamentioning

confidence: 99%

“…[1][2][3][4] FA is involved in the formation of red blood cells, as well as the acquisition, transport and enzymatic processing of one carbon unit for amino acid and nucleic acid metabolism. 5,6 Meanwhile, many reports have demonstrated that FA and vitamin B 12 play essential roles in the synthesis of DNA and RNA.…”

Section: Introductionmentioning

confidence: 99%

11-Mercaptoundecanoic acid capped gold nanoclusters as a fluorescent probe for specific detection of folic acid via a ratiometric fluorescence strategy

et al. 2018

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“…This improves the sensitivity of the assay and allows to reduce the reaction times and to use smaller volumes of solution on the working electrode surface. Moreover, numerous standard procedures for the functionalization of the MBs with different linkage groups of distinct nature are available [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Screen‐Printed Electrochemical 96‐Well Plate: a High‐Throughput Platform for Multiple Analytical Applications

et al. 2014

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A new screen‐printed electrochemical array formed by 96 three‐electrode electrochemical cells with carbon‐based working electrodes (DRP‐96X110) was developed by DropSens. The electrochemical and electro(analytical) performance of this multiple‐analysis plate is herein presented. Different proofs of concept relying on reference benchmark redox compounds, streptavidin‐biotin high affinity interactions and magnetic‐based immunoassays were carried out. The results showed the electrochemical plate versatility and usefulness as an innovative high‐throughput transducer surface for multiple and decentralized applications.

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“…Recently, it has been developed different composite materials based on different types of polymeric matrices, obtaining interesting electrochemical applications by using graphite powder as conductive phase [1,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Influence of raw carbon nanotubes diameter for the optimization of the load composition ratio in epoxy amperometric composite sensors

et al. 2014

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In this work, it is reported the necessity to characterize the raw carbon materials before their application in composite electrodes based on multiwall carbon nanotubes (MWCNTs) dispersed in epoxy resin for the development of improved amperometric sensors. These sensors must contain an optimum MWCNT/epoxy ratio for their best electroanalytical response. The main drawback in MWCNTs composite materials resides in the lack of homogeneity of the different commercial nanotubes largely due to different impurities content, as well as dispersion in their diameter/length ratio and state of aggregation. The optimal composite electrode composition takes into account the high electrode sensitivity, low limit of detection, fast response, and electroanalytical reproducibility. These features depend on carbon nanotube physical properties as the diameter. Three different commercial carbon nanotubes with different diameters were characterized by transmission electron microscopy and the results were significantly different from the ones provided by the manufacturers. Then, the three MWCNTs were used for the MWCNT/epoxy sensors construction. After an accurate electrochemical characterization by cyclic voltammetry and electrochemical impedance spectroscopy, they were employed as working electrodes using ascorbic acid as a reference analyte. Percolation theory was applied in order to verify the electrochemical results. It is demonstrated that the optimum interval load of raw carbon material in the optimized-composite electrodes closely depends on the MWCNTs diameter, needing 5 % in carbon content for the narrowest MWCNTs containing composite electrodes versus 12 % for the widest MWCNTs.

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Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors

Cited by 78 publications

References 20 publications

11-Mercaptoundecanoic acid capped gold nanoclusters as a fluorescent probe for specific detection of folic acid via a ratiometric fluorescence strategy

11-Mercaptoundecanoic acid capped gold nanoclusters as a fluorescent probe for specific detection of folic acid via a ratiometric fluorescence strategy

Screen‐Printed Electrochemical 96‐Well Plate: a High‐Throughput Platform for Multiple Analytical Applications

Influence of raw carbon nanotubes diameter for the optimization of the load composition ratio in epoxy amperometric composite sensors

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