Immunoassay detection of tumor-associated autoantibodies using protein G bioconjugated to nanomagnet-silica decorated with Au@Pd nanoparticles

Adeniyi, Omotayo; Sicwetsha, Simbongile; Adesina, Abiola Olanike; Mashazi, Philani

doi:10.1016/j.talanta.2021.122127

Cited by 12 publications

(9 citation statements)

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“…. The fabrication of Au‐PEAmbutylAc was reported [40–43] for the immobilization of p53 antigen. The amide coupling reaction was used for the covalent attachment of the 4‐aminophenylboronic (APBA).…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of amine functionalized magnetic-silica nanoparticles has been reported before [41,42] and is shown in the Supplementary Information (SI). In this work, the subsequent steps involved the synthesis of 4-(5carboxypentylamido)phenylboronic acid (3) from 4-aminophenylboronic acid (1) and glutaric anhydride (2) as Scheme 1.…”

Section: Preparation Of Anti-crp-pab Magnetic-silica Nanoparticles Sc...mentioning

confidence: 99%

“…The fabrication of Au-PEAmbutylAmPBA-anti-CRP-mAb/glucose immunosensor was followed as shown in Scheme 2. The fabrication of Au-PEAmbutylAc was reported [40][41][42][43] for the immobilization of p53 antigen. The amide coupling reaction was used for the covalent attachment of the 4-aminophenylboronic (APBA).…”

Section: Fabrication Of Immunosensor Au-peambutylampba-anti-crp-mab/g...mentioning

confidence: 99%

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Nanomagnet Bioconjugates with anti‐CRP Polyclonal Antibodies as Nanobioprobes for Enhanced Impedimetric Detection of CRP

2022

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Selective purification of biological materials for their detection in complex sample matrix is a general challenge for many researchers working in the field of diagnostics. Magnetic nanoparticles functionalized with biological molecules that impart biomolecular selectivity are therefore of major interest as capture probes thus allowing for magnetic separations. Nanoparticles can also be used for the enhanced detection of biomarkers. In this work, an ultrasensitive sandwich-based impedimetric immunosensor was fabricated for the detection of Creactive protein antigen (CRPAg). Stable and oriented immobilization of anti-CRP monoclonal antibody was achieved onto electrografted phenylethylamine derivat-ized with succinic anhydride and phenylboronic acid via carbodiimide chemistry. The detection of CRPAg was achieved using Electrochemical Impedance Spectroscopy (EIS). The enhancement of the impedance charge-transfer resistance (R CT ) signal was achieved using the sandwich approach. The anti-CRP polyclonal antibody was immobilized in an oriented manner onto magnetic nanoparticles functionalized with phenylboronic acid. The increase in the change in charge-transfer resistance (ΔR CT ) values was linearly proportional to the concentration of CRPAg in the range 10 to 200 ng mL À 1 covering the clinical range for CRPAg detection and with a detection limit of 0.34 ng mL À 1 .

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Anti-crp-pab Magnetic-silica Nanoparticles Sc...mentioning

confidence: 99%

Section: Fabrication Of Immunosensor Au-peambutylampba-anti-crp-mab/g...mentioning

confidence: 99%

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Nanomagnet Bioconjugates with anti‐CRP Polyclonal Antibodies as Nanobioprobes for Enhanced Impedimetric Detection of CRP

2022

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“…Sometimes, nanozymes themselves also show ultraviolet–visible (UV–vis) absorption, generating a high background signal and a low signal-to-noise (S/N) ratio. Therefore, detecting the change in UV–vis absorption (ΔA) is necessary [ 27 , 28 ]. A colorimetric immunosensor was developed for the detection of tumor-associated anti-p53 autoantibodies (antip53aAbs), using protein G functionalized nanomagnet-silica nanoparticles decorated with Au@Pd (Fe 3 O 4 @SiO 2 -NH 2 -Au@Pd 0.30 NPs-protG) as the nano-biological probe.…”

Section: Immunoassay Based On Nanozymesmentioning

confidence: 99%

“…A colorimetric immunosensor was developed for the detection of tumor-associated anti-p53 autoantibodies (antip53aAbs), using protein G functionalized nanomagnet-silica nanoparticles decorated with Au@Pd (Fe 3 O 4 @SiO 2 -NH 2 -Au@Pd 0.30 NPs-protG) as the nano-biological probe. Fe 3 O 4 @SiO 2 -NH 2 -Au@Pd 0.30 NPs-protG not only had high affinity for the captured anti-P53AAbs but also exhibited a great catalytic performance for the oxidation of 3,3,5, 5-tetramethylbenzidine (TMB) [ 27 ].…”

Section: Immunoassay Based On Nanozymesmentioning

confidence: 99%

Recent Advances in the Immunoassays Based on Nanozymes

et al. 2022

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As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to the merits of high specificity and sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay, which can provide high detection sensitivity since the enzyme labels can promote the generation of catalytically amplified readouts. However, the natural enzyme labels usually suffer from low stability, high cost, and difficult storage. Inspired by the advantages of superior and tunable catalytic activities, easy preparation, low cost, and high stability, nanozymes have arisen to replace the natural enzymes in immunoassay; they also possess equivalent sensitivity and selectivity, as well as robustness. Up to now, various kinds of nanozymes, including mimic peroxidase, oxidase, and phosphatase, have been incorporated to construct immunosensors. Herein, the development of immunoassays based on nanozymes with various types of detection signals are highlighted and discussed in detail. Furthermore, the challenges and perspectives of the design of novel nanozymes for widespread applications are discussed.

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Electrochemical detection of the cardiac biomarker cardiac troponin I

Qin,

Li,

Qin

et al. 2024

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Acute myocardial infarction (AMI) is a major cause of cardiovascular disease‐related death. It is essential for patients with cardiovascular disease to receive an early diagnosis of AMI. The most popular technique for the early detection of AMI is the use of biosensors to monitor the concentration of pertinent biomarkers, such as cardiac troponin I (cTnI), in the blood. The electrochemical detection methods hold great promise because of their simplicity, miniaturization, ease of integration, high sensitivity, and rapid response. The prime motive of this review is to present a comprehensive understanding of the pros and cons of methodologies employed for the electrochemical approaches toward the detection of cTnI. A detailed summary is provided for the immunosensors, aptamer sensors, molecular imprinting sensors, and peptide sensors based on various affinity elements. We enumerate the modified electrode materials for electrochemical sensors as well as popular detection techniques. Furthermore, this paper reviews some recent significant advances in point‐of‐care assays for rapid, accurate detection of cTnI as a smart integrated device for home monitoring. The accumulation of knowledge about these functions will lead to new insights into and concepts for the design of portable miniature sensors for cardiovascular patients at risk of AMI. It is anticipated that the interdisciplinary collaboration can bring more enlightenment to the progress of cardiac biomarkers sensor in the future.

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Immunoassay detection of tumor-associated autoantibodies using protein G bioconjugated to nanomagnet-silica decorated with Au@Pd nanoparticles

Cited by 12 publications

References 50 publications

Nanomagnet Bioconjugates with anti‐CRP Polyclonal Antibodies as Nanobioprobes for Enhanced Impedimetric Detection of CRP

Nanomagnet Bioconjugates with anti‐CRP Polyclonal Antibodies as Nanobioprobes for Enhanced Impedimetric Detection of CRP

Recent Advances in the Immunoassays Based on Nanozymes

Electrochemical detection of the cardiac biomarker cardiac troponin I

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