Immunoaffinity pre-column for selective on-line sample pre-treatment in high-performance liquid chromatography determination of 19-nortestosterone

Farjam, A.; Jong, Gerhardus J. de; Frei, R.W.; Brinkman, U.A.Th.; Haasnoot, W.; Hamers, A.R.M.; Schilt, R.; Huf, F.A.

doi:10.1016/s0021-9673(01)81465-5

Cited by 78 publications

(18 citation statements)

References 13 publications

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“…The hyphenation of IA-SPE with LC [27] and GC [28] in determination of some anabolic steroids, though not testosterone, in bovine urine has been presented. In this study, we prepared a testosterone-specific immunosorbent to extract testosterone in male urine.…”

Section: Introductionmentioning

confidence: 99%

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

et al. 2007

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Conventional methods for the determination of testosterone in body fluids typically suffer from poor recovery, lack of specificity, complex sample pretreatment, or the need for derivatization. Here, a simple, specific, and fast analysis method for testosterone was developed, with a methodology based on testosterone-specific immunoaffinity SPE (IA-SPE) and subsequent analysis by partial filling MEKC (PF-MEKC). An immunosorbent consisting of a recombinant antitestosterone Fab fragment covalently attached to activated Sepharose was prepared. IA-SPE and PF-MEKC were set up in hyphenated and off-line constructions, and the applicability of the two constructions in analysis of testosterone in male urine was investigated. The results obtained with the hyphenated construction proved to be only indicative of the presence of testosterone. The off-line IA-SPE and PF-MEKC construction, however, was successfully used in the determination of free testosterone in male urine samples after enzymatic hydrolysis of the glucuronide conjugates. Except for the hydrolysis reaction, no sample pretreatment was required. After hydrolysis, the overall analysis time per sample was only 14 min. The off-line IA-SPE and PF-MEKC method proved to be robust, sensitive (LOQ 35 mg/L), and specific, enabling separation of testosterone from four related steroids. Thus, it provides attractive features when compared to traditional methods for determination of testosterone in male urine.

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Section: Introductionmentioning

confidence: 99%

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

et al. 2007

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“…Analyte-antibody interactions were used to design immunosorbent, which can be used as a classical SPE material. In this approach antibody produced against a target compound is immobilized on a solid phase to achieve selective extraction [92][93][94][95][96][97]. One group of classical-type SPE system includes the use of macrocyclic chelants, such as crown ethers, immobilized on a silica or polymer support which was designed to attain selectivity in the separation process [98][99][100][101][102][103][104][105][106][107].…”

Section: Development Of Spe Materials: Activated Carbon To Macrocyclesmentioning

confidence: 99%

Selective separation of elements from complex solution matrix with molecular recognition plus macrocycles attached to a solid-phase: A review

Rahman

Begum

Hasegawa

2013

Microchemical Journal

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Microchemical Journal, 110: 485-493, 2013 The original publication is available at: http://dx.doi. org/10.1016/j.microc.2013.06.006 Abstract Solid-phase extraction (SPE) approach was introduced approximately five decades ago, and until then development of SPE materials is seamlessly continued. Lately, the SPE-based research is increasingly focused in developing more explicit materials to achieve meticulous separation of elements from complex solution matrices with high concentrations of interfering ions. One group of SPE materials includes those with macrocyclic ligands immobilized on a solid-phase, which are capable of selective separation and preconcentration of elements, and such selectivity in metal retention is generally termed as molecular recognition. In the process, the designed 'host' material possesses a high degree of recognition to specific elements or groups of elements called 'guest', and the recognition capability remains effective at the very low concentrations of the 'guest' species or when those present in complex matrices. The routes to the development of element-selective SPEs, the operating principles, applications and limitations are discussed in this review. KeywordsSeparation; element-selectivity; Macrocycles; Solid-phase extraction; Molecular recognition 2 Microchemical Journal, 110: 485-493, 2013 The original publication is available at: http://dx.doi.org/10.1016/j.microc.2013.06.006 IntroductionAlthough metals and metalloids are ubiquitous in nature, the environmental concentrations of both toxic and essential elements have been largely increased mostly pursuant to anthropogenic activities related to the industrial development and improved living standards in modern societies. The growing extent of metal pollution also initiated a number of legislative measures, such as, RoHS or ELV directive has been imposed to specify the limit of trace elements in the industrial products, while the EU directive defined the acceptable concentrations of different elements in cultivable soils.Sensitive analytical techniques such as, flame atomic absorption spectrometry, electrothermal atomic absorption spectrometry, inductively coupled plasma mass spectrometry, inductively coupled plasma optical emission spectrometry, and so forth are available for precise analysis of trace elements in solution. An overall analytical process comprises a number of succeeding steps, including sampling, sample preparation, separation and quantification. Among the aforementioned steps, sample preparation is by far the most important error source in modern analytical method development due to the low species concentration or heterogeneous distribution of the analytes in the matrix as well as the complex nature of the sample matrices. Therefore, a clean-up/separation step is often recommended before the analytical determination of trace elements in effluent or industrial waste waters to avoid the interfering effect from the matrix ions or to facilitate preconcentration due to their low concentrations in samples. The t...

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“…Improved selectivity can be obtained by introducing proper flushing steps d~dng desorption where undesirable compounds are eluted prior to or after the analytes of interest. The use of more selective sorbents containing, e.g., immobilized antibodies [68] is a good alternative.…”

Section: Solidphase Extraction-liquid Desorptionmentioning

confidence: 99%

Trace level analysis of micropollutants in aqueous samples using gas chromatography with on-line sample enrichment and large volume injection

Mol

Janssen

Cramers

et al. 1995

Journal of Chromatography A

106

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Immunoaffinity pre-column for selective on-line sample pre-treatment in high-performance liquid chromatography determination of 19-nortestosterone

Cited by 78 publications

References 13 publications

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

Selective separation of elements from complex solution matrix with molecular recognition plus macrocycles attached to a solid-phase: A review

Trace level analysis of micropollutants in aqueous samples using gas chromatography with on-line sample enrichment and large volume injection

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