Immunoaffinity beads for selective removal of cholesterol from human plasma

Abstract

Anti-low density lipoprotein antibody (anti-LDL antibody) attached poly(2-hydroxyethyl methacrylate-methacryloylamidophenylalanine) (poly(HEMA-MAPA)) beads were prepared for selective removal of cholesterol from hypercholesterolemic human plasma. Poly(HEMA-MAPA) beads were produced by a modified suspension polymerization and then characterized by swelling tests and SEM. Blood-compatibility tests were also investigated. The water swelling ratio of the poly(HEMA-MAPA) beads increased significantly (68%) compared with pHEMA (55%). All the clotting times increased when compared with poly(HEMA) beads. Loss of platelets and leukocytes was very low. The maximum anti-LDL antibody attachment was achieved at pH 7.0. Attachment of anti-LDL antibody was 29.6 mg/g. There was a very low non-specific cholesterol binding onto the poly(HEMA-MAPA) beads, about 0.74 mg/g. Anti-LDL antibody attached beads adsorbed in the range of 13.3-16.0 mg cholesterol/g from hypercholesterolemic human plasma. Up to 92% of the adsorbed LDL was desorbed. The binding-elution cycle was repeated 10 times using the same beads. There was no significant loss of binding capacity.

All-in-one paper-based sampling chip for targeted protein analysis

“…Additionally, immunoaffinity 90 and MIP 91 have been demonstrated in on-line LCMS systems making for automated and highly selective sample preparation. Other platforms have also been introduced such as chip-based MIP 92 and immunoaffinity 93 , immunoaffinity pipette tips (MSIA) 94 , beads 89,[95][96][97] and 96well plates 98 .…”

Considerations for non-invasive in-flight monitoring of astronaut immune status with potential use of MEMS and NEMS devices

“…The nanoparticles used for probing and labeling include quantum dots, colloidal gold and silver, polymer beads, dye-doped nanoparticles, and organic nanoparticles (Seydack, 2005). Out of these, quantum dots (which are semiconducting light-emitting nanocrystals) and polymer beads have been widely used in the study of cancer (Gao et al, 2004;Sage, 2004), bacteria (Wenhua et al, 2004), immunological and cellular interactions (Michalet et al, 2005;Stsiapura et al, 2004;Yavuz and Denizli, 2003), and multiplexed detection.…”

Hydrophilic Core‐Shell Microspheres: A Suitable Support for Controlled Attachment of Proteins and Biomedical Diagnostics

“…Yavuz et al prepared poly(2‐hydroxyethylmethacrylate‐methacryloylamidophenylalanine) (poly(HEMA‐MAPA)) microspheres with attached antibodies against low density lipoprotein (anti‐LDL antibody) and used them for the selective removal of cholesterol from hypercholesterolemic human plasma 85. Particles with a surface concentration of anti‐LDL antibody equal to 29.6 mg · g −1 were used for the removal of cholesterol.…”