Immunoadsorption with protein A sepharose or silica

Belák, M; Widder, RandolfA; Brunner, Richard; Borberg, H.; Haupt, WalterF

doi:10.1016/s0140-6736(94)91868-6

Cited by 16 publications

(4 citation statements)

References 3 publications

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“…Severe side effects did not occur. They are not typical for adsorption therapy in general and if they occur they appear to depend on the character of the different materials used [20].…”

Section: Discussionmentioning

confidence: 99%

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Widder¹,

Brunner²,

Engels³

et al. 1998

Blood Purif

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Background: Selective adsorption is an extracorporeal treatment able to reduce high-molecular-weight proteins and lipids. We evaluated its efficacy in lowering hemorheological parameters to achieve a better microcirculation of the retina. Patients and Methods: Ten patients suffering from maculopathies of various origin underwent a selective plasma adsorption procedure using the TR-350. Plasma and whole blood viscosity, erythrocyte aggregation and proteins and lipids were determined before and 24 h after therapy. Results: Selective adsorption therapy reduced the high-molecular-weight proteins and lipids. Plasma viscosity, standardized whole blood viscosity and erythrocyte aggregation were significantly lowered to 87, 88 and 65%, respectively, of their values prior to treatment. An improvement of visual acuity was achieved in 6/10 patients. Minor side effects were noted in 2/10 patients. Conclusions: Selective adsorption using the TR-350 adsorber is a safe technique, showing a high impact on blood rheology. The changes of hemorheological parameters led to clinical improvement in 6/10 patients suffering from retinal disorders.

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“…Severe side effects did not occur. They are not typical for adsorption therapy in general and if they occur they appear to depend on the character of the different materials used [20].…”

Section: Discussionmentioning

confidence: 99%

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Widder¹,

Brunner²,

Engels³

et al. 1998

Blood Purif

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“…Плазма, очищена від імуноглобулінів, повертається пацієнту. При проходженні через колонку для А-ІА одного об'єму циркулюючої плазми видаляється близько 90 % імуноглобулінів класу G і приблизно 55 % імуноглобулінів класів M і A. При цьому не спостерігається значне зниження рівня фібриногену [14,15].…”

Section: основні методи елімінації анти-A/b антитілunclassified

Methods of anti-A/B antibodies elimination in preparation for ABО incompatible kidney transplantation

Zograbian

Zakordonetz

Malyk

et al. 2021

KIDNEYS

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Kidney transplantation is considered the gold standard in the treatment of end-stage renal disease and can be performed if cadaveric or living related donor organ is available. AB0 incompatible kidney transplantation can lead to antibody-mediated rejection. There are a number of methods in the world for the elimination of anti-A/B antibodies to prepare for AB0 incompatible kidney transplantation, each of which has its advantages and disadvantages. Therefore, further researches are needed in this direction to determine the optimal method in each case. Particular attention is paid to modern methods of anti-A/B antibodies elimination, protocols for managing such patients, and their comparison. The possibilities of using these methods in Ukraine are analyzed.

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“…1,2 Protein A immunoadsorption column composes of an activated carrier and protein A, which is bonding to the surface of the carrier. Agarose gel is the carrier of protein A immunoadsorption column and its activation reaction process in the stirred reactor is the key to achieve larger scale industrial production of blood purification materials.…”

Section: Introductionmentioning

confidence: 99%

Simulation of Solid–liquid Suspension and Scale-Up of Agarose Gel Activation Reactor

Zhou

Liu²,

Yu³

et al. 2016

J. Mech. Med. Biol.

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Agarose gel activation reaction, which is of great importance in preparing the carrier of the column packing material for blood purification, would be significantly influenced by the configuration and parameter of reactor. In order to optimize the structure design of the reactor and operating parameters of the process, the characteristics of suspension system composed of agarose gel, NaOH, water, 3-allyl bromide, and activated alumina were simulated numerically utilizing an Eulerian multiphase flow model and multi-reference frame (MRF) approach. The effect of impeller configuration was studied with three typical impellers, including Rushton disk turbine (DT), pitched blade downflow turbine (PBTD45), and pitched blade upflow turbine (PBTU45). The results showed that the optimum solid suspension was obtained using PBTD45 impeller with a diameter of 100 mm and critical suspension speed of 570 rpm in a 20 L stirred reactor. In addition, the critical suspension speeds using the three impellers were calculated § Corresponding author. and the errors were all within the engineering allowance. Finally, the feasibility of scaleup design for agarose gel activation reactor was preliminarily discussed. The results would be useful to the optimization and scale-up of relevant reactor design. NomenclatureB : Impeller width, mm C : Impeller off-bottom clearance, mm C D0 : Drag coefficient in still liquid C D : Drag coefficient in turbulent liquid d P : Particle diameter, m D : Impeller diameter, mm H : Liquid height, mm k : Turbulent kinetic energy, m 2 /s 2

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Immunoadsorption with protein A sepharose or silica

Cited by 16 publications

References 3 publications

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Methods of anti-A/B antibodies elimination in preparation for ABО incompatible kidney transplantation

Simulation of Solid–liquid Suspension and Scale-Up of Agarose Gel Activation Reactor

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