Immuno-impedimetric Biosensor for Onsite Monitoring of Ascospores and Forecasting of Sclerotinia Stem Rot of Canola

Shoute, Lian C. T.; Anwar, Afreen; MacKay, Scott; Abdelrasoul, Gaser N.; Lin, Donghai; Yan, Zhimin; Nguyen, Anh H.; McDermott, Mark T.; Shah, Manzoor Ahmad; Yang, Jian; Chen, Jie; Li, Xiujie S.

doi:10.1038/s41598-018-30167-5

Cited by 17 publications

(14 citation statements)

References 51 publications

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“…Although PCR can achieve the detection of a few nanograms of S. sclerotiorum DNA, its complex operation and instrumentation hinder their potential application in the field . Our proposed method is simple, portable, and sensitive to single spores, which can allow us to provide an accurate and early warning system to avoid SSR outbreaks.…”

Section: Resultsmentioning

confidence: 99%

“…Nano- and micromachined devices emerge as an alternative to provide such a platform. 20 , 24 In this study, we designed a microfluidic device based on a nanoelectrode-activated microwell array for the capture and quantification of S. sclerotiorum spores. Microwell arrays have been commonly used for high-throughput cell sequencing 25 − 29 and cell pairing, 30 , 31 as well as for cancer cell identification and characterization.…”

Section: Introductionmentioning

confidence: 99%

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Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

et al. 2021

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In this study, we present a microdevice for the capture and quantification of Sclerotinia sclerotiorum spores, pathogenic agents of one of the most harmful infectious diseases of crops, Sclerotinia stem rot. The early prognosis of an outbreak is critical to avoid severe economic losses and can be achieved by the detection of a small number of airborne spores. However, the current lack of simple and effective methods to quantify fungal airborne pathogens has hindered the development of an accurate early warning system. We developed a device that remedies these limitations based on a microfluidic design that contains a nanothick aluminum electrode structure integrated with a picoliter well array for dielectrophoresis-driven capture of spores and on-chip quantitative detection employing impedimetric sensing. Based on experimental results, we demonstrated a highly efficient spore trapping rate of more than 90% with an effective impedimetric sensing method that allowed the spore quantification of each column in the array and achieved a sensitivity of 2%/spore at 5 kHz and 1.6%/spore at 20 kHz, enabling single spore detection. We envision that our device will contribute to the development of a low-cost microfluidic platform that could be integrated into an infectious plant disease forecasting tool for crop protection.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

et al. 2021

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“…EIS’s working principle is based on the measure of the opposition to the flow of the current that results from redox reactions and molecular interactions at the electrode surface when voltage is applied to the cell membrane ( Shoute et al, 2018 ). Khater et al recently developed a labelless impedimetric biosensor for the detect the nucleic acid of citrus tristeza virus and the experiment demonstrated that this method of a high potential can be used in fields to detect plant pathogens.…”

Section: Biosensor Technologies For Plant Pathogen Detectionmentioning

confidence: 99%

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

et al. 2021

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Plant pathogens are a major reason of reduced crop productivity and may lead to a shortage of food for both human and animal consumption. Although chemical control remains the main method to reduce foliar fungal disease incidence, frequent use can lead to loss of susceptibility in the fungal population. Furthermore, over-spraying can cause environmental contamination and poses a heavy financial burden on growers. To prevent or control disease epidemics, it is important for growers to be able to detect causal pathogen accurately, sensitively, and rapidly, so that the best practice disease management strategies can be chosen and enacted. To reach this goal, many culture-dependent, biochemical, and molecular methods have been developed for plant pathogen detection. However, these methods lack accuracy, specificity, reliability, and rapidity, and they are generally not suitable for in-situ analysis. Accordingly, there is strong interest in developing biosensing systems for early and accurate pathogen detection. There is also great scope to translate innovative nanoparticle-based biosensor approaches developed initially for human disease diagnostics for early detection of plant disease-causing pathogens. In this review, we compare conventional methods used in plant disease diagnostics with new sensing technologies in particular with deeper focus on electrochemical and optical biosensors that may be applied for plant pathogen detection and management. In addition, we discuss challenges facing biosensors and new capability the technology provides to informing disease management strategies.

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“…As previously mentioned, conventional laboratory techniques have the sensitivity and specificity for the detection of microbial pathogens in water. However, due to lack of rapid analysis, overall cost, and experimental complexity they are unsuitable for the purpose of on-site detection [113]. Nonetheless, the development of truly portable and autonomous diagnostic devices remains a major task.…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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Immuno-impedimetric Biosensor for Onsite Monitoring of Ascospores and Forecasting of Sclerotinia Stem Rot of Canola

Cited by 17 publications

References 51 publications

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

Biosensor Technologies for Early Detection and Quantification of Plant Pathogens

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

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