Immuno-hematologic parameters following rodent spinal cord contusion are negatively influenced by high-fat diet consumption

Person, Jon M.; Welch, Bradley A.; Spann, Redin A.; Harris, Kwamie K.; Pride, Yilianys; Tucci, Michelle; Taylor, Erin B.; Grayson, Bernadette E.

doi:10.1016/j.jneuroim.2020.577226

Cited by 4 publications

(7 citation statements)

References 40 publications

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“… 13 , 29 These metabolic parameters are worsened with consumption of HFD, 13 and saturated fat consumption is further damaging to the immune system after SCI. 15 We have previously reported the chronic impact SCI has on circulating peripheral leukocytes and immune organs such as the spleen and thymus. 15 The complex way in which metabolic health and inflammation are interrelated suggest that improving metabolic health would have direct and indirect positive influence on immune health and vice versa.…”

Section: Discussionmentioning

confidence: 99%

“… 15 We have previously reported the chronic impact SCI has on circulating peripheral leukocytes and immune organs such as the spleen and thymus. 15 The complex way in which metabolic health and inflammation are interrelated suggest that improving metabolic health would have direct and indirect positive influence on immune health and vice versa. Obtaining a clear view of the markers that could inform us concerning their temporal progression could refine holistic health management of the SCI individual and provide new avenues of intervention for long-term care.…”

Section: Discussionmentioning

confidence: 99%

“…tSCI surgeries were performed as previously described. 13 - 15 Briefly, following exposure of the thoracic level 10 (T10) spinal cord, a laminectomy was performed while grasping the ventral surface of the lateral spinous processes at vertebral levels T9 and T11. Using an Infinite Horizon Spinal Impactor Device (Precision Systems and Instrumentation, LLC), moderate contusion/compression injuries were delivered to the T10 spinal cord using 150 kdynes of force with a 1-second dwell.…”

Section: Methodsmentioning

confidence: 99%

“…As previously described, 13 , 15 rats received one dose of buprenorphine SR (sustained release; 1.0 to 1.2 mg/kg SQ; ZooPharm) and, 72 hours later, single-dose buprenorphine for postsurgical pain management (0.025 mg/kg, twice daily for a period of 2 days, then as needed). In addition, rats also receive (1) antibiotic, Naxcel (5 mg/kg SQ, Zoetis) once daily for a period of 5 days, and (2) 3 to 5 mL of 0.9% saline, twice daily for a period of 3 days to ensure hydration.…”

Section: Methodsmentioning

confidence: 99%

“…These were performed as previously described. 13,15 Body Weight and Composition Following surgery, animals were weighed daily for the first 14 days and then weekly after that. Lean and fat body mass composition was analyzed using Echo Magnetic Resonance Imaging (echoMRI; EchoMedical Systems) before euthanasia for each cohort.…”

Section: Hindlimb Locomotor Function Assessmentmentioning

confidence: 99%

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Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion

Santos

Welch

Edwards

et al. 2020

Global Spine Journal

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Study Design: Basic science animal research study. Objectives: Using T10 spinal contused rats, we sought to identify molecular and circulating, metabolic and immune biomarkers during the subchronic and chronic recovery periods that may inform us concerning neurorehabilitation. Methods: Gene expression of the cord and ELISA were performed in 28 and 100 days in T10 injured rats and compared to sham-injured rats. Hundred-day injured rats were placed on either a low-fat or high-fat diet following the recovery phase. Linear regression analysis was performed between markers and locomotor score, body weight, body composition, and blood cholesterol and triglycerides. Results: Gene expression in the thoracic cord for complement marker, C1QC, dendritic cell marker, ITGAX, and cholesterol biosynthesis genes, FDFT1, HMCGR, LDLR, and SREBP1, were significantly associated with BBB score, body weight, composition, and other metabolic parameters. Circulating levels of these proteins, however, did not vary by injury or predict the level of locomotor recovery. Conclusions: Identification of reliable circulating biomarkers that are durable and based on level of spinal injury are complicated by immune and metabolic comorbidities. Continued work is necessary to identify stable markers of disease progression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Hindlimb Locomotor Function Assessmentmentioning

confidence: 99%

See 3 more Smart Citations

Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion

Santos

Welch

Edwards

et al. 2020

Global Spine Journal

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Aging with spinal cord injury: A narrative review of consequences and challenges

Guı́zar-Sahagún¹,

Grijalva²,

Franco-Bourland³

et al. 2023

Ageing Research Reviews

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Altered chronic glycemic control in a clinically relevant model of rat thoracic spinal contusion

et al. 2022

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The lifetime risk for Type-2 diabetes mellitus remains higher in people with spinal cord injuries than in the able-bodied population. However, the mechanisms driving this disparity remain poorly understood. The goal of the current study was to evaluate the impact of a palatable high-fat diet (HFD) on glycemic regulation using a rodent model of moderate thoracic contusion. Animals were placed on either Chow or HFD and tolerance to glucose, insulin, and ENSURE mixed meal were investigated. Important targets in the gut-brain axis were investigated. HFD consumption equally induced weight gain in SCI and naïve rats over CH rats. Elevated blood glucose was observed during intraperitoneal GTT in HFD-fed rats over CH-fed rats. ITT was unremarkable amongst the three groups. Gavage of ENSURE resulted in high GLP-1 release from SCI rats over naïve controls. An elevation in terminal total GLP-1 was measured, with a marked reduction in circulating dipeptidyl peptidase 4 (DPP4), the GLP-1 cleaving enzyme, in SCI rats, compared to naïve. Increased glucagon mRNA in the pancreas and reduced immunoreactive glucagon-positive staining in the pancreas in SCI rats compared to controls suggested increased glucagon turnover. Finally, GLP-1 receptor gene expression in the ileum, the primary source of GLP-1 production and release, in SCI rats suggests the responsivity of the gut to altered circulating GLP-1 in the body. In conclusion, the actions of GLP-1 and its preprohormone, glucagon, are markedly uncoupled from their actions on glucose control in the SCI rat. More work is required to understand GLP-1 in the human.

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Immuno-hematologic parameters following rodent spinal cord contusion are negatively influenced by high-fat diet consumption

Cited by 4 publications

References 40 publications

Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion

Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion

Aging with spinal cord injury: A narrative review of consequences and challenges

Altered chronic glycemic control in a clinically relevant model of rat thoracic spinal contusion

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