Immuno-enhancement effects of Yifei Tongluo Granules on cyclophosphamide-induced immunosuppression in Balb/c mice

Wang, Yongjie; Qi, Qiuchen; Li, Ang; Yang, Min; Huang, Weizhen; Xu, Hongya; Zhao, Zhongxi; Li, Siying

doi:10.1016/j.jep.2016.08.046

Cited by 72 publications

(52 citation statements)

References 48 publications

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“…Similar changes were also shown in the present Cy-induced mouse model, indicating that the immunosuppression model was built successfully. The spleen is an important immune organ in nonspecific immune regulation, where the T and B cells colonize, synthesize biologically active substances, and produce an immune response [25]. Cy injection caused a decrease in the spleen index, suggesting that the immune function of spleen decreased, and the spleen itself shrank.…”

Section: Discussionmentioning

confidence: 99%

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

et al. 2020

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Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions. Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear. Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model. In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined. The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates. Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin. For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α). The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis. SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus). The results of this study suggest an innovative application of citrus pectin as an immunomodulator.

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Section: Discussionmentioning

confidence: 99%

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

et al. 2020

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“…To investigate whether the LVFP possessed immunomodulatory functions, a Cy-induced immunosuppressed mouse model was established. Cy is a chemotherapeutic agent and has been used to establish immunosuppressive animal models [32,33]. At the level of immune organs, the results indicated that the LVFP significantly increased spleen and thymus indexes in Cy-induced immunosuppressive mice.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit

Liu

Wang

Ren

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Ligustrum vicaryi L. is a hybrid of Ligustrum ovalifolium Hassk. var. aureo-marginatum and Ligustrum vulgale L., belonging to the Oleaceae family. It is often used as an ornamental shrub due to its golden leaves. However, its medical value is yet to be discovered. Recently, plant polysaccharides have attracted comprehensive attention owing to their biological properties, including immunomodulatory and antioxidant activities. This study aimed to extract, purify, and characterize the polysaccharide from the Ligustrum vicaryi L. fruit and investigate its immunomodulatory and antioxidant activities. The Ligustrum vicaryi L. fruit polysaccharide (LVFP) was obtained by ultrasonic extraction, ethanol precipitation, macroporous resin separation, and dialysis bag purification. The physicochemical properties of the LVFP were elucidated using Fourier-transform infrared spectrometry, high-performance ion chromatography, and high-performance gel filtration chromatography. The results indicated that the LVFP consisted of rhamnose, arabinose, galactose, and glucose in a ratio of 1.79 : 7.55 : 4.58 : 1.54, and its molecular weight was 88,949 Da. The immunomodulatory and antioxidant activities of the LVFP were investigated using a cyclophosphamide- (Cy-) induced immunosuppressed mouse model. The results demonstrated that the LVFP significantly increased spleen and thymus indexes, enhanced the phagocytic function of neutrophils, activated B and T lymphocytes, and upregulated serum levels of IL-10 and TNF-α. Moreover, we observed that the LVFP relieved Cy-induced liver damage by increasing superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) levels. These results suggested that the LVFP has the immunomodulatory and antioxidant activities, therefore laying a foundation for the application of the LVFP in the pharmaceutical and functional food industries.

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“…The YFTL extract is provided by Dr. Yanke Liu, Changsha Central Hospital (161 Shaoshan Rd. S, Yuhua Dist, Changsha China) and prepared as described in [ 19 ]. The conversion of the human equivalent dose (HED) to the mouse equivalent dose (MED) was calculated according to a previous study by using the following formula for dose translation based on body surface area: HED (mg/kg) = MED (mg/kg) × (Mouse km/Human km), in which mouse km = 3 and human (adult) km = 37.…”

Section: Methodsmentioning

confidence: 99%

“…Yifei Tongluo (YFTL) is a traditional Chinese medicine (TCM) formulation that contains multiple herbs for balance of Yin and Yang, a system of two opposites but complementary aspects of nature to maintain immune homeostasis. Treatment with YFTL with the combination of chemotherapy agents has improved the clinical symptoms of patients with MDR-TB in China [ 19 – 21 ]. However, the mechanisms underlying YFTL treatment of TB have not been investigated although a study shows that YFTL enhances the activities of natural killer cells and balances the ratio of Th1/Th2 cells of immune deficient naïve mice [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Treatment with YFTL with the combination of chemotherapy agents has improved the clinical symptoms of patients with MDR-TB in China [ 19 – 21 ]. However, the mechanisms underlying YFTL treatment of TB have not been investigated although a study shows that YFTL enhances the activities of natural killer cells and balances the ratio of Th1/Th2 cells of immune deficient naïve mice [ 19 ]. The purpose of this study is to verify the effects of YFTL on mycobacterial infection and dissect the mechanisms underlying the clinical improvement with a mouse model infected with Bacillus Calmette-Guérin (BCG).…”

Section: Introductionmentioning

confidence: 99%

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Protective immune mechanisms of Yifei Tongluo, a Chinese herb formulation, in the treatment of mycobacterial infection

Fan

Wang

et al. 2018

PLoS ONE

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Yifei Tongluo (YFTL) is a traditional Chinese medicine (TCM) formulation which has been shown clinical efficacy in treatment of patients with multidrug-resistant tuberculosis in China. However, the underlying mechanisms of the effects of YFTL are lacking. This study investigated the effects of YFTL on immune regulation with a mouse lung infection model with Bacille Calmette-Guérin (BCG). We found that compared with untreated mice, the lung mycobacterial load in YFTL-treated mice was significantly reduced, accompanied by alleviated pulmonary inflammation with reduction of pro-inflammatory cytokines and increase of prostaglandin E2 (PGE2). Flow cytometry analyses showed that Th1 cells were significantly higher in the lungs of YFTL-treated mice at early infection time. The results suggest that YFTL-treatment down-regulates pulmonary inflammation, which facilitates a rapid infiltration of Th1 cells into the lungs. Moreover, the Th1 cells in the lungs were resolved faster at later time concomitant with increased the regulatory T cells (Tregs). The reduction of mycobacterial burden associated with improved tissue pathology, faster Th1 cell trafficking, and accelerated resolution of Th1 cells in the lungs of YFTL-treated mice indicates that YFTL improves mycobacterial clearance by maintaining lung homeostasis and dynamically regulating T cells in the lung parenchyma, and suggests that YFTL can be used as host-directed therapies that target immune responses to mycobacterial infection.

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Immuno-enhancement effects of Yifei Tongluo Granules on cyclophosphamide-induced immunosuppression in Balb/c mice

Cited by 72 publications

References 48 publications

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit

Protective immune mechanisms of Yifei Tongluo, a Chinese herb formulation, in the treatment of mycobacterial infection

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