Immunization with recombinant ORF2 p551 protein protects common marmosets (Callithrix jacchus) against homologous and heterologous hepatitis E virus challenge

Gordeychuk, Ilya; Kyuregyan, Karen K.; Kondrashova, A. S.; Bayurova, Ekaterina; Gulyaev, Stanislav A.; Gulyaeva, Tatiana V.; Potemkin, Ilya А.; Karlsen, Anastasia A.; Isaeva, Olga V.; Belyakova, A. V.; Lyashenko, Anna; Sorokin, Aleksei; Chumakov, Alexey; Ia, Morozov; Isaguliants, Maria; Ishmukhametov, Aydar A.; Михайлов, М. И.

doi:10.1016/j.vaccine.2021.11.042

Cited by 5 publications

(3 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to natural susceptibility to different hepatotropic viruses such as hepatitis E virus [ 10 ], and other viruses causing liver pathology such as lymphocytic choriomeningitis virus (causative agent of Callitrichid hepatitis) [ 36 ] and Lassa virus [ 37 ], the development of CM hepatocytes culture is an important task. Primary CM hepatocyte cultures were obtained in 1991 by Stephensen et al Cells were characterized by stable expression of apolipoproteins Al and E for over 66 days in culture and were used to characterize Callitrichid Hepatitis associated virus as the etiologic agent of Callitrichid hepatitis [ 38 ].…”

Section: Primary CM Cellsmentioning

confidence: 99%

“…CMs are naturally susceptible to multiple human viral, protozoan and bacterial pathogens [ 7 ] including Yellow fever [ 8 ], Epstein-Barr virus (EBV) and other Herpesviruses, hepatitis A virus [ 9 ], Junin virus, malaria, measles, hepatitis E virus [ 10 ]. With multiple methods of assessment of humoral and cell-based immune response developed in recent years, this makes CMs an optimal model for preclinical studies of safety and efficacy of novel regimens of prophylaxis and treatment of infectious diseases, including vaccines, immunotherapeutics and cell-based therapies [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Common Marmoset Cell Lines and Their Applications in Biomedical Research

Bayurova,

Zhitkevich,

Avdoshina

et al. 2023

Cells

Self Cite

View full text Add to dashboard Cite

Common marmosets (Callithrix jacchus; CMs) are small New World primates widely used in biomedical research. Early stages of such research often include in vitro experiments which require standardized and well-characterized CM cell cultures derived from different tissues. Despite the long history of laboratory work with CMs and high translational potential of such studies, the number of available standardized, well-defined, stable, and validated CM cell lines is still small. While primary cells and immortalized cell lines are mostly used for the studies of infectious diseases, biochemical research, and targeted gene therapy, the main current applications of CM embryonic stem cells and induced pluripotent stem cells are regenerative medicine, stem cell research, generation of transgenic CMs, transplantology, cell therapy, reproductive physiology, oncology, and neurodegenerative diseases. In this review we summarize the data on the main advantages, drawbacks and research applications of CM cell lines published to date including primary cells, immortalized cell lines, lymphoblastoid cell lines, embryonic stem cells, and induced pluripotent stem cells.

show abstract

Section: Primary CM Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Common Marmoset Cell Lines and Their Applications in Biomedical Research

Bayurova,

Zhitkevich,

Avdoshina

et al. 2023

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…The sequence heterogenicity of HVR is significantly associated with the number of hosts (Purdy et al., 2012). ORF2 encodes the capsid protein that is crucial for the assembly of viral particles (Li et al., 1997) and mediates the HEV binding and infection of cells, making it an effective target for vaccine development (Gordeychuk et al., 2022; Jimenez de Oya et al., 2012; Mazalovska & Kouokam, 2020). ORF3 encodes a cytoskeleton‐associated phosphoprotein (Zafrullah et al., 1997) and is responsible for efficiently releasing the virion from HEV‐infected cells (Ding et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Genomic characteristics and recombination patterns of swine hepatitis E virus in China

Bahoussi

Guo

Wang

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

Zoonotic hepatitis E, mainly caused by swine hepatitis E virus (sHEV), is endemic in China, causing great economic disruption and public health threats. Although recombination is critical for the evolution of viruses, there is a limited assessment of its occurrence among sHEVs. Herein, we analysed all available sHEV full-length genomes isolated in China during the past two decades (40 isolates) compared to 72 other sHEV strains isolated in different countries and determined that sHEV genotype 4 (sHEV4) dominates China. Eight potential natural recombination events were identified, four of which occurred in China and were mainly between sHEV4 strains, indicating the distinct character of China sHEV. One intergenotype recombination event was found in China, alarming the emergence of a new sHEV lineage that could become a critical threat to human health.

show abstract

Experimental Use of Common Marmosets (<i>Callithrix jacchus</i>) in Preclinical Trials of Antiviral Vaccines

Gordeychuk,

Gancharova,

Gulyaev

et al. 2024

Acta Naturae

View full text Add to dashboard Cite

Common marmoset (Callithrix jacchus, CM) is a New World primate species that is of interest for preclinical trials of immunobiological products. In this study, we describe the approaches to long-term laboratory breeding and maintenance of CMs. We also establish the reference values of the main complete blood count and serum chemistry parameters evaluated during preclinical trials of immunobiological products and describe the histological characteristics of CM lymphoid organs during the development of post-vaccination immune response. We show that CMs bred in laboratory conditions excluding background infectious pathology are a relevant model that allows for a high degree of reliability in characterizing the safety and immunogenicity profile of antiviral vaccines during preclinical trials.

show abstract

Immunization with recombinant ORF2 p551 protein protects common marmosets (Callithrix jacchus) against homologous and heterologous hepatitis E virus challenge

Cited by 5 publications

References 71 publications

Common Marmoset Cell Lines and Their Applications in Biomedical Research

Common Marmoset Cell Lines and Their Applications in Biomedical Research

Genomic characteristics and recombination patterns of swine hepatitis E virus in China

Experimental Use of Common Marmosets (<i>Callithrix jacchus</i>) in Preclinical Trials of Antiviral Vaccines

Contact Info

Product

Resources

About