Immunization with Peptide-Functionalized Carbon Nanotubes Enhances Virus-Specific Neutralizing Antibody Responses

Pantarotto, Davide; Partidos, Charalambos D.; Hoebeke, Johan; Brown, F.; Kramer, Ed; Briand, Jean‐Paul; Muller, Sylviane; Prato, Maurizio; Bianco, Alberto

doi:10.1016/j.chembiol.2003.09.011

Cited by 497 publications

(292 citation statements)

References 38 publications

(1 reference statement)

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“…If an antigen is linked to a CNT, the resulting construct could be more easily phagocytosed and rapidly introduced into the immune system, thus improving the antibody response. Pantarotto et al [53,54] demonstrated the potential of using CNTs for animal 90 immunization. In their seminal work, a peptide from the foot-and-mouth disease virus (FMDV) was covalently coupled to amino-derivatized single-walled carbon nanotubes (SWNTs), and this conjugate was administered to mice.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their 25 properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strobilurin fungicide, as a model analyte. An azoxystrobin derivative bearing a carboxylated spacer arm (hapten AZc6) was covalently coupled to bovine serum albumin (BSA), and the resulting BSA-AZc6 conjugate was 30 covalently linked to four functionalized CNTs of different shapes and sizes, varying in diameter and length. These four types of CNT-based constructs were obtained using efficient, fast, and easy functionalization procedures based on microwave-assisted chemistry. New Zealand rabbits and BALB/c mice were immunized with BSA-AZc6 alone and with the four CNT-BSA-AZc6 constructs, both with and without Freund's adjuvant. The IgG-type antibody responses were assessed 35 in terms of the titer and affinity, paying special attention to the relationship between the immune response and the size and shape of the employed CNTs. Immunization with CNT-BSA-AZc6 resulted in enhanced titers and excellent affinities for azoxystrobin. More important, remarkable IgG responses were obtained even in the absence of an adjuvant, thus proving the self-adjuvanting capability of CNTs. Immunogens were able to produce strong anti-azoxystrobin immune responses 40 in rabbits even when administered at a BSA-AZc6 conjugate dose as low as 0.05 g. The short and thick CNT-BSA-AZc6 construct produced the best antibody response under all tested conditions.

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Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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“…Efforts have been made to assess cytotoxicity and to comprehensively enhance CNT biocompatibility. Cellular assays have been studied extensively using several cell types such as skin (HaCaT), lung (A549), pharynx (FaDu), blood-cell elements (macrophages), kidneys (HEK293) and disease-specific animal models such as human volunteers, rats, pigs, mice and rabbits [18][19][20][21][22][23][24][25][26][27][28][29]. Skin, lungs and blood-borne cells are relatively more challenging but are easily accessible organs in a given CNT-polluted environment [30].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity, biophysics and biomechanics of cells treated with functionalized hybrid nanomaterials

Subbiah

Ramasundaram

et al. 2013

J. R. Soc. Interface.

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Hybrids consisting of carboxylated, single-walled carbon nanotube (c-SWNT)-silver nanoparticles (AgNPs)-DNA-poly vinyl alcohol (PVA) are synthesized via sequential functionalization to mimic the theragnostic (therapy and diagnosis) system. Carboxylation of SWNT has minimized the metal impurities with plenty of -COOH groups to produce hybrid (c-SWNT-AgNPs). The hybrid is further wrapped with DNA (hybrid-DNA) and encapsulated with PVA as hybrid composite (HC). Materials were tested against human alveolar epithelial cells (A549), mouse fibroblasts cells (NIH3T3) and human bone marrow stromal cells (HS-5). The composition-sensitive physico-chemical interactions, biophysics and biomechanics of materials-treated cells are evaluated. The cell viability was improved for HC, hybrid-PVA and c-SWNT when compared with SWNT and hybrid. SWNT and hybrid showed cell viability less than 60% at high dose (40 mg ml 21 ) and hybrid-PVA and HC retained 80% or more cell viability. The treatment of hybrid nanomaterials considerably changed cell morphology and intercellular interaction with respect to the composition of materials. Peculiarly, PVA-coated hybrid was found to minimize the growth of invadopodia of A549 cells, which is responsible for the proliferation of cancer cells. Surface roughness of cells increased after treatment with hybrid, where cytoplasmic regions specifically showed higher roughness. Nanoindentation results suggest that changes in biomechanics occurred owing to possible internalization of the hybrid. The changes in force spectra of treated cells indicated a possible greater interaction between the cells and hybrid with distinct stiffness and demonstrated the surface adherence and internalization of hybrid on or inside the cells.

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“…Both methods demonstrated that the SWNTs-linked peptide retained the structural features required for recognition by anti-FMDV peptide monoclonal and polyclonal antibodies [148,211].…”

Section: Presentation and Immunogenic Potential Of Peptide Antigens Amentioning

confidence: 99%

Carbon Nanotube‐based Vectors for Delivering Immunotherapeutics and Drugs

Bianco

Pastorin

et al. 2003

Nanotechnologies for the Life Sciences

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Immunization with Peptide-Functionalized Carbon Nanotubes Enhances Virus-Specific Neutralizing Antibody Responses

Cited by 497 publications

References 38 publications

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Evaluation of cytotoxicity, biophysics and biomechanics of cells treated with functionalized hybrid nanomaterials

Carbon Nanotube‐based Vectors for Delivering Immunotherapeutics and Drugs

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