Immunization with Outer Membrane Proteins of Pasteurella multocida (6:B) Provides Protection in Mice

Abstract

The immunoprotective efficacy of Pasteurella multocida (6:B) outer membrane proteins (OMPs) was examined in the mouse model. Bacterial OMPs were extracted using sarkosyl method and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. Prototype vaccines were prepared using OMPs with adjuvants including dioleoyl phosphatidyl choline-based liposome and Montanide ISA206 water-in oil-in water emulsion. Antibody response to the vaccine was monitored using indirect enzyme linked immunosorbent assay. The results of the study showed that immunized mice had high titre with both the formulations. The vaccinated mice were able to survive a live virulent bacterial challenge. Based on the findings of the study it can be inferred that OMPs are important determinants of immunoprotection hence can serve as vaccine candidates against haemorrhagic septicaemia.

Outer Membrane Proteome Analysis of Indian Strain ofPasteurella multocidaSerotype B:2 by MALDI-TOF/MS Analysis

“…However, the 125 kDa protein which is only present in iron-limited condition and 70 kDa protein which is common for both iron-limited and iron-rich condition were found to be immunodominant with hyperimmune sera that rose against P. multocida serotype B:2 in bovine calves. More or less similar observations have been reported in the western blot analysis by previous workers [ 8 , 19 , 24 – 26 , 31 , 37 ]. 32 kDa and 37 kDa protein have been reported to be the most immunogenic proteins of P. multocida serotype B isolates.…”

“…32 kDa and 37 kDa protein have been reported to be the most immunogenic proteins of P. multocida serotype B isolates. Except for these proteins, some authors also reported 16, 20, 28, 44, 48, 50, 56, 86, and 90 kDa proteins to be immunogenic [ 8 , 19 , 24 – 26 , 31 , 37 ]. These differences in the observations could be due to the different serotypes used in the study and/or individual animal factors in eliciting the immune response to these proteins.…”

“…The key antigens of P. multocida B:2 that evoke protective immunity against HS in cattle have still not been well defined, but its OMPs have been found as protective antigens [ 6 , 8 , 9 ]. Boyce et al [ 5 ] have studied the OMPs of P. multocida during infection of the natural host in chickens and by subjecting sarcosine-insoluble membrane fractions to 2-DE and 1-DE followed by MALDI-TOF/MS and nano-LC MS/MS analysis and have identified 35 proteins.…”

Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice

“…Oil-based adjuvants (emulsions) have been used in vaccine formulations since 1945 (Aucouturier et al, 2006). Montanide ISA 206 is a safe water-in-oil-in-water emulsion commonly used in vaccine studies against animal pathogens including P. multocida (Basagoudanavar et al, 2006;Dupuis et al, 2006). It has been shown that CpG ODN in combination with Montanide ISA 206 synergistically induced higher immune responses to VP1 antigen of FMDV in mice and cattle as compared to an either CpG ODN or Montanide only formulation (Ren et al, 2011).…”

“…Recent studies have shown that several P. multocida outer membrane proteins (OMPs) contributed to the pathogenesis and possess immunogenic and bactericidal properties (Basagoudanavar et al, 2006;Lee et al, 2007;Tan et al, 2010). OmpH is an antigenic, surface-exposed and conserved OMP porin that is detected in 100% of bovine isolates investigated and has been mooted as a potential vaccine candidate (Dabo et al, 2008b).…”