Immunization with newly transformed <i>Schistosoma mansoni</i> schistosomula tegument elicits tegument damage, reduction in egg and parasite burden

Melo, Tatiane Teixeira de; Araújo, Juliano Michel de; Durães, Fernanda do Valle; Caliari, Marcelo Vidigal; Oliveira, Sérgio C.; Coelho, Paulo Marcos Zech; Fonseca, Cristina Toscano

doi:10.1111/j.1365-3024.2010.01244.x

Cited by 30 publications

(23 citation statements)

References 50 publications

(52 reference statements)

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“…As mentioned earlier, the tegument is a promising source of potential targets for the development of an antischistosomiasis vaccine. In a recent publication, it was reported that a preparation containing tegument antigens extracted from S. mansoni schistosomula when used as vaccine in mice elicited reduction in the number of eggs trapped in the liver, the number of eggs eliminated in the faeces, the number of hepatic granulomas and parasite burden [19]. However, the work by Teixeira de Melo et al [19] is different from our study since we are using here only GPI-anchored proteins and not the entire tegument.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent publication, it was reported that a preparation containing tegument antigens extracted from S. mansoni schistosomula when used as vaccine in mice elicited reduction in the number of eggs trapped in the liver, the number of eggs eliminated in the faeces, the number of hepatic granulomas and parasite burden [19]. However, the work by Teixeira de Melo et al [19] is different from our study since we are using here only GPI-anchored proteins and not the entire tegument. Several tegument and tegument-associated proteins have been evaluated as vaccine candidates in the last years, such as Sm29 [9], TSP2 [8], Sm22.6 [23], a Stomatin-Like Protein-2 [24], Sm-p80 (a subunit of calpain) [25], ECL (Sm200) [26], Sm25 [27, 28] among many others (for a better review see Pinheiro et al 2011 [29]).…”

Section: Discussionmentioning

confidence: 99%

“…The use of preparations containing tegument proteins as antigens to immunize and protect mice was earlier shown to be feasible [19]. Herein, we evaluated the potential of GPI-anchored proteins of S. mansoni to elicit an immunological response able to protect mice against cercarial challenge and reduce the pathology associated with schistosomiasis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Vaccination with Enzymatically Cleaved GPI-Anchored Proteins fromSchistosoma mansoniInduces Protection against Challenge Infection

Martins

Pinheiro

Figueiredo

et al. 2012

Clinical and Developmental Immunology

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The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vaccination with Enzymatically Cleaved GPI-Anchored Proteins fromSchistosoma mansoniInduces Protection against Challenge Infection

Martins

Pinheiro

Figueiredo

et al. 2012

Clinical and Developmental Immunology

View full text Add to dashboard Cite

show abstract

“…A strong evidence of the potentiality of S. mansoni tegument-bound antigens as vaccine candidates was recently demonstrated using an extract of tegument proteins from lung-stage schistosomula named Smteg [77]. Smteg-immunized mice showed a Th1 type of immune response associated with 48% reduction in worm burden, 65% reduction in liver eggs, 60% reduction in fecal eggs, and 41% reduction in liver granuloma [77].…”

Section: Tegument Proteins As Vaccine Candidatesmentioning

confidence: 99%

“…Smteg-immunized mice showed a Th1 type of immune response associated with 48% reduction in worm burden, 65% reduction in liver eggs, 60% reduction in fecal eggs, and 41% reduction in liver granuloma [77]. …”

Section: Tegument Proteins As Vaccine Candidatesmentioning

confidence: 99%

Computational Vaccinology: An Important Strategy to Discover New PotentialS. mansoniVaccine Candidates

Pinheiro

Martins

Assis

et al. 2011

Journal of Biomedicine and Biotechnology

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The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. Several papers on Schistosoma mansoni vaccine and drug development have been published in the past few years, representing an important field of study. The advent of technologies that allow large-scale studies of genes and proteins had a remarkable impact on the screening of new and potential vaccine candidates in schistosomiasis. In this postgenomic scenario, bioinformatic technologies have emerged as important tools to mine transcriptomic, genomic, and proteomic databases. These new perspectives are leading to a new round of rational vaccine development. Herein, we discuss different strategies to identify potential S. mansoni vaccine candidates using computational vaccinology.

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Molecular characterization and identification of Th1 epitopes of a Schistosoma japonicum protein similar to prosaposin

Zhang

Zhao

et al. 2013

Parasitol Res

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The tegument of schistosomula contains T cell antigens that might simulate the protective mechanisms of the radiation-attenuated vaccine in a mouse model of schistosomiasis. Immune mechanisms mediated by the CD4+ Th1 response are important in the RAV model. To rapidly identify Th1 epitopes in molecules from the Schistosoma japonicum schistosomula tegument, this study analyzed S. japonicum proteomics data. Preliminary experiments identified a protein similar to prosaposin (SjPSAP) from the tegument of schistosomula. We confirmed that SjPSAP was present in the tegument of the parasite using an indirect immunofluorescence assay. We then identified Th cell epitopes in SjPSAP using in silico prediction combined with experimental validation. From the SjPSAP sequence, we used several algorithms to predict 11 promiscuous Th cell epitopes that might bind to both murine and human MHC class II molecules. To validate the in silico predictions, proliferation and cytokine production profiles of spleen lymphocytes from BALB/c mice immunized with the 11 predicted peptides were measured in vitro using a modified methyl thiazolyl tetrazolium assay and flow cytometry. The results showed that 4 of the 11 predicted peptides induced a recall CD4+ Th1 response in vitro. We measured direct binding of the four peptides predicted to induce a response to antigen-presenting cells from BALB/c mice using a fluorometric method and found that the peptides bound to both I-Ad and I-Ed mouse molecules. These results demonstrated that potentially protective Th1-type epitopes in SjPSAP molecules could be identified rapidly by combining in silico prediction with experimental validation. This strategy could be a fast method for identifying Th1 epitopes in a schistosoma antigen with features such as large size or poor expression of recombinant antigens.

show abstract

Immunization with newly transformed Schistosoma mansoni schistosomula tegument elicits tegument damage, reduction in egg and parasite burden

Cited by 30 publications

References 50 publications

Vaccination with Enzymatically Cleaved GPI-Anchored Proteins fromSchistosoma mansoniInduces Protection against Challenge Infection

Vaccination with Enzymatically Cleaved GPI-Anchored Proteins fromSchistosoma mansoniInduces Protection against Challenge Infection

Computational Vaccinology: An Important Strategy to Discover New PotentialS. mansoniVaccine Candidates

Molecular characterization and identification of Th1 epitopes of a Schistosoma japonicum protein similar to prosaposin

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