Immunization with autologous CD46 generates a strong autoantibody response in rats that targets spermatozoa

Mizuno, Masashi; Harris, Claire L.; Morgan, B. Paul

doi:10.1016/j.jri.2006.08.001

Cited by 8 publications

(6 citation statements)

References 46 publications

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“…Its specific expression in the epididymis and its demonstrated role in the initiation of sperm maturation makes Bin1b a promising candidate for male contraception. The present findings are consistent with several recent studies showing that inhibition of the epididymal ß-defensin molecules in the rat, mouse, or monkey through immunization can inhibit sperm function and fertility (5,(13)(14)(15) and indicating the potential of Bin1b as an immunocontraceptive.…”

Section: Figuresupporting

confidence: 93%

Immunization with Bin1b decreases sperm motility with compromised fertility in rats

Zhang

Chen

et al. 2010

Fertility and Sterility

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Section: Figuresupporting

confidence: 93%

Immunization with Bin1b decreases sperm motility with compromised fertility in rats

Zhang

Chen

et al. 2010

Fertility and Sterility

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“…This high-titre polyclonal antiserum was affinity-purified against non-conjugated peptide immobilised to an agarose gel support using a Sulfolink kit (Perbio Science, Cramlington, UK) and eluted with 100 mM glycine buffer (pH2.8). A rat anti-rat CD46 polyclonal antiserum generated using a recombinant fusion protein comprising IgG-Fc and rat SCR2-3 CD46 domains was a kind gift of B. P. Morgan, C. L. Harris and M. Mizuno, Cardiff University School of Medicine [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

Clift

Andrlikova

Frolikova

et al. 2009

Reprod Biol Endocrinol

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Background: In rodents, the cell surface complement regulatory protein CD46 is expressed solely on the spermatozoal acrosome membrane. Ablation of the CD46 gene is associated with a faster acrosome reaction. Sperm from Apodemus flavicollis (yellow-necked field mice), A. microps (pygmy field mice) and A. sylvaticus (European wood mice) fail to express CD46 protein and exhibit a more rapid acrosome reaction rate than Mus (house mice) or BALB/c mice. A. agrarius (striped field mice) belong to a different Apodemus subgenus and have pronounced promiscuity and large relative testis size. The aim of this study was to determine whether A. agrarius sperm fail to express CD46 protein and, if so, whether A. agrarius have a faster acrosome reaction than Mus.

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“…The immune antiserum gave an OD 405 nm 1.40G0.01 at 1:3200 dilution in ELISA against BSApeptide, whereas it was 0.11G0.01 at the same dilution against BSA alone; pre-immune serum gave OD 405 nm values of 0.13G0.01 and 0.12G0.01 at 1:3200 dilution against BSA-peptide and BSA alone respectively. We also included a polyclonal rat anti-rat CD46 antiserum (Mizuno et al 2007).…”

Section: Cd46 Protein Is Not Expressed In a Sylvaticus Testis Or Spermmentioning

confidence: 99%

“…A polyclonal antiserum raised against a recombinant rat CD46 SCR123 fusion protein by immunisation of female rats was a kind gift of B P Morgan, C L Harris and M Mizuno, Cardiff University School of Medicine (Mizuno et al 2007). Seven separate mouse monoclonal antibodies (MM1,2,3,4,5,8 and 9) raised against the rat CD46 SCR123 fusion protein (Mizuno et al 2004 were also a kind gift of B P Morgan, C L Harris and M Mizuno.…”

Section: Antibodiesmentioning

confidence: 99%

“…Thus, CD46 protein is expressed in rodents only by mature sperm and spermatozoal precursors in testes with active spermatogenesis (Mizuno et al 2004). Additionally, anti-CD46 antibody may not block in vivo fertilisation in the rat (Mizuno et al 2007). The CD46 gene in rodents contains 11 functional exons, in comparison with 14 in humans, and includes a single STP and a single CYTexon; alternative splicing of rodent membrane-bound CD46 has rarely been described.…”

Section: Introductionmentioning

confidence: 99%

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Rapid sperm acrosome reaction in the absence of acrosomal CD46 expression in promiscuous field mice (Apodemus)

Johnson

Clift²,

Andrlikova³

et al. 2007

Reproduction

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There is pronounced promiscuity and sperm competition in long-tailed field mice (Apodemus sylvaticus). These mice have evolved unusual sperm behaviour favouring rapid fertilisation, including dynamic formation of sperm trains and their subsequent dissociation. The cell surface complement regulatory (CReg) protein CD46 is broadly expressed in eutherian mammals other than rodents, in which it is expressed solely on the spermatozoal acrosomal membrane. Ablation of the CD46 gene has been associated with a faster acrosome reaction (AR) rate in inbred laboratory mice. Here, we demonstrate that wild-caught field mice of three species, A. sylvaticus, A. flavicollis and A. microps, exhibit a more rapid AR than wild-caught house mice Mus musculus or inbred laboratory BALB/c mice. We also demonstrate that wild-caught field mice of these three species, unlike house mice, produce alternatively spliced transcripts of testicular CD46 mRNA lacking exons 5-7 or 6-7, together with an extended 3 0 -and often truncated 5 0 -utr, leading to failure to express any sperm CD46 protein in both the testis and epididymis. Male field mice may therefore have traded expression of this CReg protein for acrosomal instability, providing a novel genus-specific strategy to favour rapid fertilisation and competitive advantage in the promiscuous reproductive behaviour of wild field mice.

show abstract

Immunization with autologous CD46 generates a strong autoantibody response in rats that targets spermatozoa

Cited by 8 publications

References 46 publications

Immunization with Bin1b decreases sperm motility with compromised fertility in rats

Immunization with Bin1b decreases sperm motility with compromised fertility in rats

Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

Rapid sperm acrosome reaction in the absence of acrosomal CD46 expression in promiscuous field mice (Apodemus)

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