Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

Crocquet‐Valdes, Patricia A.; Díaz-Montero, C. Marcela; Feng, Hui; Li, H.; Barrett, Alan D.T.; Walker, David H.

doi:10.1016/s0264-410x(01)00377-2

Cited by 68 publications

(48 citation statements)

References 31 publications

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“…As expected, OmpA and OmpB were identified as major antigens and as surface-exposed proteins in the present study. It has been shown that both proteins are able to stimulate protective immunity against rickettsiosis in laboratory animals (6,9,36,41). To the best of our knowledge, the immunogenicity of the remaining three antigenic proteinstranslation initiation factor IF-2, cell division protein FtsZ, and cysteinyl-tRNA synthetase-has not been described for other Rickettsia spp.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Rickettsia parkeri Strain Portsmouth

et al. 2009

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Rickettsia parkeri, a recently recognized pathogen of human, is one of several Rickettsia spp. in the United States that causes a spotted fever rickettsiosis. To gain insights into its biology and pathogenesis, we applied the proteomics approach to establish a two-dimensional gel proteome reference map and combined this technique with cell surface biotinylation to identify surface-exposed proteins of a low-passage isolate of R. parkeri obtained from a patient. We identified 91 proteins by matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry. Of these, 28 were characterized as surface proteins, including virulence-related proteins (e.g., outer membrane protein A [OmpA], OmpB, ␤-peptide, and RickA). Two-dimensional immunoblotting with serum from the R. parkeri-infected index patient was utilized to identify the immunoreactive proteins as potential targets for diagnosis and vaccine development. In addition to the known rickettsial antigens, OmpA and OmpB, we identified translation initiation factor 2, cell division protein FtsZ, and cysteinyl-tRNA synthetase as immunoreactive proteins. The proteome map with corresponding cell surface protein analysis and antigen detection will facilitate a better understanding of the mechanisms of rickettsial pathogenesis.Rickettsia parkeri, a member of the spotted fever group Rickettsia (SFGR), was first isolated from the Gulf Coast tick, Amblyomma maculatum, in 1937 (29). In 2004, the first confirmed human infection with R. parkeri was reported in a 40-year-old man from the Tidewater area of coastal Virginia. The agent was isolated in cell culture from an eschar biopsy specimen and designated the Portsmouth strain (28). Recently, the first recognized case of tick bite-associated human infection was described (43); however, the epidemiology of R. parkeri is not well defined. In the United States, R. parkeri has been detected in A. maculatum and A. americanum; the geographical overlap between R. parkeri and these ticks with that of the vectors of R. rickettsii (the etiological agent of Rocky Mountain spotted fever [RMSF]) suggests that many cases of R. parkeri infection have been misidentified as RMSF (27,35). For example, Western blot analysis of serum specimens from 15 U.S. patients previously diagnosed with RMSF identified four serum specimens reactive with a 120-kDa protein of R. parkeri, suggesting infection with R. parkeri rather than R. rickettsii (30). However, a serologic test specific for this pathogen is not available (43), and little is known about its biology.Due to their obligate intracellular nature, genetic manipulation of Rickettsia has proven difficult. Alternatively, protein expression profiles (proteomes) are utilized to identify the mechanisms of pathogenesis and differentiate rickettsial species recognizing host immune response specificity to cell surface molecules, referred to as outer membrane proteins (Omps). The presence or absence of some Omps allows for differentiation between the typhus group and the SFGR, and the response...

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Rickettsia parkeri Strain Portsmouth

et al. 2009

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“…Crocquet-Valdes et al (2001) demonstraram o potencial imunoestimulador da proteína rOmpA ao utiliza-la como vacina recombinante em camundongos, onde os mesmos apresentaram uma significativa resposta imune ao serem desafiados com R. conorii. De maneira semelhante, Sumner et al (1995) inocularam uma proteína recombinante de rOmpA em cobaias, os quais desenvolveram anticorpos reativos contra R. rickettsii.…”

Section: Discussionunclassified

Detecção de proteínas imunorreativas de Rickettsia sp. cepa Mata Atlântica

et al. 2017

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RESUMO.-A Febre Maculosa Brasileira (FMB) é uma doença infecciosa, transmitida por carrapatos ao homem. Uma nova riquetsiose humana foi descrita como causadora de Febre Maculosa no Estado de São Paulo, sendo denominada de Rickettsia sp. cepa Mata Atlântica. O presente trabalho teve como objetivo detectar e identificar proteínas com potencial de estimular o sistema imune de hospedeiro mamí-fero, desta nova cepa descrita. Para tanto, foi realizado a extração proteica total de Rickettsia sp. cepa Mata Atlântica. As proteínas extraídas foram fracionadas por eletroforese. As bandas proteicas foram transferidas para membranas de nitrocelulose por migração elétrica e submetidas à téc-nica de Western-blot, para detecção proteica. Ao todo sete proteínas imunorreativas foram detectadas. Duas proteínas apresentaram maior abundancia, com peso molecular, de 200 e 130 kDa respectivamente. Através da comparação de mapas proteômicos existentes e pelo peso molecular que estas proteínas apresentaram, sugere-se que as duas proteínas detectadas representem rOmpA (200 kDa) e rOmpB (130 kDa). As demais proteínas detectadas apresentaram menor ocorrência e peso molecular inferior a 78 kDa, podendo representar membros da família de antígenos de superfície celular (Sca -Surface cell antigen). As proteínas detectadas poderão servir como base de estudo na elabo-

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“…15 OmpA-and OmpBinduced protective immunity involves both complement-mediated killing in mammalian blood 12 and the activation of antigenspecific memory CD4 C and CD8 C T cells. 13 YbgF and TolC have been previously identified as major SEPs in rickettsiae. YbgF, a periplasmic protein, is an abundant surface protein and major sero-reactive antigen of rickettsiae.…”

Section: Discussionmentioning

confidence: 99%

“…11 Both OmpA and OmpB have the ability to elicit efficient protection against R. rickettsii infection. 12,13 Other Sca proteins have not been demonstrated to be protective antigens of R. rickettsii.…”

Section: Introductionmentioning

confidence: 99%

Rickettsia rickettsiiouter membrane protein YbgF induces protective immunity in C3H/HeN mice

Gong

Xiong

et al. 2015

Human Vaccines & Immunotherapeutics

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Rickettsia rickettsii is the etiological agent of Rocky Mountain spotted fever (RMSF). YbgF and TolC are outer membrane-associated proteins of R. rickettsii that play important roles in its interaction with host cells. We investigated the immunogenicity of YbgF and TolC for protection against RMSF. We immunized C3H/HeN mice with recombinant R. rickettsii YbgF (rYbgF) or TolC (rTolC). Rickettsial burden and impairment in the lungs, spleens, and livers of rYbgFimmunized mice were significantly lower than in rTolC-immunized mice. The ratio of IgG2a to IgG1 in rYbgF-immunized mice continued to increase over the course of our experiments, while that in rTolC-immunized mice was reduced. The proliferation and cytokine secretion of CD4 C and CD8 C T cells isolated from R. rickettsii-infected mice were analyzed following antigen stimulation. The results indicated that proliferation and interferon (IFN)-g secretion of CD4 C or CD8 C T cells in R. rickettsii-infected mice were significantly greater than in uninfected mice after stimulation with rYbgF. YbgF is a novel protective antigen of R. rickettsii. Protection conferred by YbgF is dependent upon IFN-g-producing CD4 C and CD8 C T cells and IgG2a, which act in synergy to control R. rickettsii infection.

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Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

Cited by 68 publications

References 31 publications

Proteomic Analysis of Rickettsia parkeri Strain Portsmouth

Proteomic Analysis of Rickettsia parkeri Strain Portsmouth

Detecção de proteínas imunorreativas de Rickettsia sp. cepa Mata Atlântica

Rickettsia rickettsiiouter membrane protein YbgF induces protective immunity in C3H/HeN mice

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