Immunization with a chimeric tobacco mosaic virus containing an epitope of outer membrane protein F of Pseudomonas aeruginosa provides protection against challenge with P. aeruginosa

Staczek, John; Bendahmane, Mohammed; Gilleland, Linda B.; Beachy, Roger N.; Gilleland, H. E.

doi:10.1016/s0264-410x(99)00571-x

Cited by 91 publications

(66 citation statements)

References 30 publications

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“…Immunization with a modified cowpea mosaic virus expressing peptide 9 or 10 induced IgG-binding antibodies against OprF and opsonizing antibodies against P. aeruginosa in mice (22). Similar results were reported with the use of OprF peptides expressed by recombinant influenza virus (53).…”

Section: Figuresupporting

confidence: 82%

Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid

Worgall¹,

Krause²,

Rivara³

et al. 2005

J. Clin. Invest.

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Pseudomonas aeruginosa is an important opportunistic pathogen that can cause chronic and often life-threatening infections of the respiratory tract, particularly in individuals with cystic fibrosis (CF). Because infections with P. aeruginosa remain the major cause of the high morbidity and mortality of CF, a vaccine against P. aeruginosa would be very useful for preventing this disorder. The outer membrane protein F (OprF) of P. aeruginosa is a promising vaccine candidate and various B cell epitopes within OprF have been identified. Given that adenovirus (Ad) vectors have strong immunogenic potential and can function as adjuvants for genetic vaccines, the present study evaluates the immunogenic and protective properties of a novel replication-deficient Ad vector in which the Ad hexon protein was modified to include a 14-amino acid epitope of P. aeruginosa OprF (Epi8) in loop 1 of the hypervariable region 5 of the hexon (AdZ.Epi8). Immunization of C57BL/6 mice with AdZ.Epi8 resulted in detectable serum anti-P. aeruginosa and anti-OprF humoral responses. These responses were haplotype dependent, with higher serum anti-OprF titers in CBA mice than in BALB/c or C57BL/6 mice. AdZ.Epi8 induced Epi8-specific IFN-γ-positive CD4 and CD8 T cell responses and resulted in protection against a lethal pulmonary challenge with agar-encapsulated P. aeruginosa. Importantly, repeated administration of AdZ.Epi8 resulted in boosting of the anti-OprF humoral and anti-Epi8 cellular response, whereas no boosting effect was present in the response against the transgene β-galactosidase. These observations suggest that Ad vectors expressing pathogen epitopes in their capsid will protect against an extracellular pathogen and will allow boosting of the epitope-specific humoral response with repeated administration, a strategy that should prove useful in developing Ad vectors as vaccines where humoral immunity will be protective.

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Section: Figuresupporting

confidence: 82%

Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid

Worgall¹,

Krause²,

Rivara³

et al. 2005

J. Clin. Invest.

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“…A significantly ( P = 0.028) higher number (61.7%) of mice immunized with CPMV-PAE5 in FCA/FIA and challenged with the FD4 immunotype strain of P. aeruginosa (Table 3) The 5 x lo3 c.f.u. cut-off has been shown previously to represent a 95% decline from the mean number of bacteria found in the lungs of challenged, control mice on day 8 after challenge in this model (Staczek et al, 1998). Since approximately 5 x lo3 c.f.u.…”

Section: Cpmv-pae5 Immunization Protects Mice From P Aeruginosa C Hamentioning

confidence: 70%

“…Pooled sera from each of the groups were examined for titres of IgG antibodies against P. aerugittosa FD2 and FD4 by ELISA as described previoiisly (Hughes et al, 1992;Staczek et al, 1998). Briefly, plates (Dynatech; Immunlon 1) were coated with a suspension of whole P. aeruginosa (FD2 or FD4) cells prepared according to the method of Abdillahi & Poolman (1987).…”

Section: Introductionmentioning

confidence: 99%

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A chimaeric plant virus vaccine protects mice against a bacterial infection

Brennan¹,

Gilleland

Staczek

et al. 1999

Microbiology

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The plant virus cowpea mosaic virus (CPMV) is an efficient carrier of foreign peptides for the generation of strong humoral immune responses. Peptides derived from both viruses and bacteria are strongly immunogenic when displayed on the surface of CPMV and elicit high titres of peptide-specific antibody. However, the protective effects of antibodies generated using bacterial epitopes in this system have yet t o be demonstrated. In this study the ability of chimaeric virus particles (CVPs) t o afford protection against bacterial infection was assessed. Immunization of outbred mice with CPMV expressing a peptide derived from outer-membrane protein F of Pseudomonas aeruginosa (CPMV-PAE5) generated high titres of P. aeruginosa-specif ic IgG that opsonized the bacteria for phagocytosis by human neutrophils and afforded protection upon challenge with t w o different immunotypes of P. aeruginosa in a model of chronic pulmonary infection. When examined 8 d after challenge, CVP-immunized mice had fewer severe lung lesions and fewer bacteria in their lungs compared t o mice immunized with wild-type virus. Different levels of protection were seen with CPMV-PAE5 when Freund's or alum adjuvants were used. These studies highlight the ability of CVPs t o generate protective immunity against infectious disease agents.

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“…The initial agar bead model of chronic bronchopulmonary infection with mucoid P. aeruginosa was modified for use in mice by Starke et al (1987) and has frequently been used to study CF lung disease, for bacterial pathogenesis (Gosselin et al 1995, Morissette et al 1995, Stevenson et al 1995, Morissette et al 1996, Moser et al 1997, Gosselin et al 1998, Sapru et al 1999, Tam et al 1999, McMorran et al 2001, Dagenais et al 2005, and for evaluation of potential new treatments (Pier et al 1990, Staczek et al 1998, van Heeckeren et al 1998, Chmiel et al 1999, Wilmott et al 2000.…”

Section: Mouse Models Of P Aeruginosa Chronic Lung Infectionmentioning

confidence: 99%

Animal models of chronic lung infection with Pseudomonas aeruginosa: useful tools for cystic fibrosis studies

Kukavica-Ibrulj

Levesque

2008

Lab Anim

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SummaryCystic fibrosis (CF) is caused by a defect in the transmembrane conductance regulator (CFTR) protein that functions as a chloride channel. Dysfunction of the CFTR protein results in salty sweat, pancreatic insufficiency, intestinal obstruction, male infertility and severe pulmonary disease. In most patients with CF life expectancy is limited due to a progressive loss of functional lung tissue. Early in life a persistent neutrophylic inflammation can be demonstrated in the airways. The cause of this inflammation, the role of CFTR and the cause of lung morbidity by different CF-specific bacteria, mostly Pseudomonas aeruginosa, are not well understood. The lack of an appropriate animal model with multi-organ pathology having the characteristics of the human form of CF has hampered our understanding of the pathobiology and chronic lung infections of the disease for many years. This review summarizes the main characteristics of CF and focuses on several available animal models that have been frequently used in CF research. A better understanding of the chronic lung infection caused particularly by P. aeruginosa, the pathophysiology of lung inflammation and the pathogenesis of lung disease necessitates animal models to understand CF, and to develop and improve treatment.

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Immunization with a chimeric tobacco mosaic virus containing an epitope of outer membrane protein F of Pseudomonas aeruginosa provides protection against challenge with P. aeruginosa

Cited by 91 publications

References 30 publications

Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid

Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid

A chimaeric plant virus vaccine protects mice against a bacterial infection

Animal models of chronic lung infection with Pseudomonas aeruginosa: useful tools for cystic fibrosis studies

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