Immunization of Rabbits with Nematode<i>Ascaris lumbricoides</i>Antigens Induces Antibodies Cross-Reactive to House Dust Mite<i>Dermatophagoides farinae</i>Antigens

Nakazawa, Takahiro; Khan, Azharul Islam; Yasueda, Hiroshi; Saito, Akemi; Fukutomi, Yuma; Takai, Toshiro; Zaman, Khalequz; Yunus, Muhammad; Takeuchi, Haruko; Iwata, Tadahisa; Akiyama, Kazuo

doi:10.1271/bbb.120626

Cited by 12 publications

(16 citation statements)

References 26 publications

(42 reference statements)

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“…Indeed IgE antibody cross reactivity has been experimentally demonstrated between the GSTs from the nematode W. bancrofti and cockroach (Bla g 5) [25]. Also, immunization of rabbits with A. lumbricoides had shown to induce cross-reactive antibodies that recognize antigens in the extract of the mite D. farinae [27]. Older studies in our laboratory showed that 10% of non-immunotherapy-treated asthmatic patients had IgE response to Schistosoma japonicum GST [28], even though this helminth does not exist in our country.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic and Immunochemical Characterization of Glutathione Transferase as a New Allergen of the Nematode Ascaris lumbricoides

et al. 2013

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Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential to understand both helminth immunity and allergy. Glutathione transferases (GSTs) from cockroach and house dust mites are clinically relevant allergens and comparative studies between them and the GST from A. lumbricoides (GSTA) are necessary to evaluate their allergenicity. We sought to analyze the allergenic potential of GSTA in connection with the IgE response to non-parasitic GSTs. IgE to purified GSTs from Ascaris (nGSTA and rGSTA), house dust mites (rDer p 8, nBlo t 8 and rBlo t 8), and cockroach (rBla g 5) was measured by ELISA in subjects from Cartagena, Colombia. Also, multidimensional proteomic approaches were used to study the extract of A. lumbricoides and investigate the existence of GST isoforms. We found that among asthmatics, the strength of IgE levels to GSTA was significantly higher than to mite and cockroach GSTs, and there was a strong positive correlation between IgE levels to these molecules.Specific IgE to GSTA was found in 13.2% of controls and 19.5% of asthmatics. In addition nGSTA induced wheal and flare in skin of sensitized asthmatics indicating that it might be of clinical relevance for some patients. Frequency and IgE levels to GSTA were higher in childhood and declined with age. At least six GST isoforms in A. lumbricoides bind human IgE. Four isoforms were the most abundant and several amino acid substitutions were found, mainly on the N-terminal domain. In conclusion, a new allergenic component of Ascaris has been discovered; it could have clinical impact in allergic patients and influence the diagnosis of mite and cockroach allergy in tropical environments.

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Section: Discussionmentioning

confidence: 99%

“…Still, its allergens have not been fully identified. There is cross reactivity among several IgE-binding components of Ascaris and other invertebrates such as domestic mites [5]–[7] and cockroaches [8]. Because of their potential impact on protective immunity to Ascaris and the pathogenesis and diagnosis of allergic diseases (e.g.…”

Section: Introductionmentioning

confidence: 99%

Proteomic and Immunochemical Characterization of Glutathione Transferase as a New Allergen of the Nematode Ascaris lumbricoides

et al. 2013

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“…[116][117][118][119][120][121][122][123][124][125][126][127][128] We have demonstrated that helminth infection can be associated with increased IgE responses to many different allergens, especially those structurally related to parasite antigens. 116 Individuals infected with filarial parasites were shown to have higher levels of IgE against HDM and cockroach extracts (that have several major allergens with homologues in filarial parasites) but not against timothy grass extract, an allergen extract with just a few minor allergen homologues in helminths.…”

Section: -89mentioning

confidence: 99%

Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond

Santiago

Nutman

2016

The American Society of Tropical Medicine and Hygiene

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Abstract. There is much debate about the interaction between helminths and allergic disease. The "Hygiene Hypothesis," a very popular concept among scientists and the lay public, states that infections, especially during childhood, can protect against allergic diseases. Indeed, helminth infections are known to induce regulatory responses in the host that can help the control of inflammation (including allergic inflammation). However, these infections also induce type-2-associated immune responses including helminth-specific IgE that can cross-react against environmental allergens and mediate IgE-driven effector responses. Thus, it is the delicate balance between the parasites' anti-and pro-allergenic effects that define the helminth/allergy interface.The immune system has evolved, in large part, through its interaction with microbes and parasites, an interaction that drives specific or specialized immune responses to deal with the widely varying groups of microorganisms. For example, parasite-derived induction of interleukin (IL)-4, IL-5, and IL-13 coordinate the prototypical responses to metazoan helminth pathogens, 1 whereas viral-and bacterial-specific induction of Type 1 and Type 2 interferons are required for control of these types of infections.

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“…A prospecmast cell degranulation [3,42]. In addition, structural and immunological similarities have been observed between allergens and parasitic antigens [75,103]. Paradoxically, even if both diseases are typically associated with Th2 polarization, epidemiological and experimental evidence shows that Th2-type allergic immune response such as sensitization to aeroallergens, airway hyperresponsivenes, eczema, rhinitis and asthma are reduced in subjects infected with helminth parasites [35,58].…”

Section: Examples Of Negative Association Between Helminth Infectionsmentioning

confidence: 99%

Allergie und Würmer: Sollen wir uns gute alte Freunde zurückholen?

Schabussova

Wiedermann

2014

Wien Med Wochenschr

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During the past 10 years, many exciting and important studies have found that certain helminth infections protect against the development of allergic diseases. Not surprisingly, several clinical trials investigated the effects of deliberate exposure to parasites like porcine whipworm (Trichuris suis) or hookworm (Necator americanus) to develop "helminth therapies". Although they proved to be a safe option to control aberrant inflammation, the final goal is to identify the parasite-derived immunnomodulatory molecules responsible for protective effects.

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Immunization of Rabbits with NematodeAscaris lumbricoidesAntigens Induces Antibodies Cross-Reactive to House Dust MiteDermatophagoides farinaeAntigens

Cited by 12 publications

References 26 publications

Proteomic and Immunochemical Characterization of Glutathione Transferase as a New Allergen of the Nematode Ascaris lumbricoides

Proteomic and Immunochemical Characterization of Glutathione Transferase as a New Allergen of the Nematode Ascaris lumbricoides

Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond

Allergie und Würmer: Sollen wir uns gute alte Freunde zurückholen?

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