Immunization of Chickens with VP2 Protein of Infectious Bursal Disease Virus Expressed in Arabidopsis thaliana

Wu, Hongzhuan; Singh, Narendra K.; Locy, Robert D.; Scissum-Gunn, Karyn; Giambrone, J. J.

doi:10.1637/7074

Cited by 41 publications

(26 citation statements)

References 22 publications

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“…Denatured VP2 does not induce protection in chickens (Fahey et al, 1989), and denatured and renatured VP2 also lost the ability to induce neutralizing antibodies in chickens (Ö ppling et al, 1989). Different expression systems have been used, such as Escherichia coli (Rong et al, 2007), yeast (Fahey et al, 1991), fowlpoxvirus (Bayliss et al, 1991;Heine & Boyle, 1993), baculovirus (Vakharia et al, 1993), Semliki Forest virus (Phenix et al, 2001), and even plant expression systems (Wu et al, 2004). Either the VP2 encoding region alone or the polyprotein gene was used for expression, and polyhistidine tags have been used for effective protein purification.…”

Section: Introductionmentioning

confidence: 99%

Current status of vaccines against infectious bursal disease

Müller

Mundt

Eterradossi³

et al. 2012

Avian Pathology

195

145

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Section: Introductionmentioning

confidence: 99%

Current status of vaccines against infectious bursal disease

Müller

Mundt

Eterradossi³

et al. 2012

Avian Pathology

195

145

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“…Plant-expressed proteins from infectious bursal disease virus and avian reovirus were previously tested successfully in chicken (29,30,31). The expression of influenza A virus antigen using plants was demonstrated recently, and protective efficacy has been investigated with mouse and ferret models (7,19,20).…”

mentioning

confidence: 99%

Immunization with Plant-Expressed Hemagglutinin Protects Chickens from Lethal Highly Pathogenic Avian Influenza Virus H5N1 Challenge Infection

Kalthoff

Giritch²,

Geisler³

et al. 2010

J Virol

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Highly pathogenic avian influenza (HPAI) is a striking disease in susceptible poultry, which leads to severe economic losses. Inactivated vaccines are the most widely used vaccines in avian influenza virus (AIV) vaccination programs. However, these vaccines interfere with the serological detection of wild-type AIV infections in immunized populations. The use of vaccines that allow differentiation between infected and vaccinated animals (DIVA strategy) would stop current stamping-out policies. Therefore, novel vaccination strategies are needed to allow improved protection of animals and humans against HPAI virus (HPAIV) infection. The presented study analyzed for the first time the immunogenic capacity of plant-expressed full-length hemagglutinin (rHA0) of HPAIV H5N1 in several vaccine formulations within the highly relevant host species chicken. We were able to express plant-expressed rHA0 at high levels and could show that, when administered with potent adjuvants, it is highly immunogenic and can fully protect chicken against lethal challenge infection. Real-time reverse transcription (RT)-PCR and serological tests demonstrated only marginally increased virus replication in animals vaccinated with plant-derived rHA0 compared to animals immunized with an inactivated reference vaccine. In addition, the use of plant-expressed rHA0 also allowed an easy serological differentiation of vaccinated from AIV-infected animals based on antibodies against the influenza virus NP protein.Highly pathogenic avian influenza (AI) (HPAI) is a striking disease in susceptible poultry, which leads to severe economic losses (21). Since 2003, the H5N1 HPAI epidemic has claimed over 220 million poultry and other birds either through direct mortality from infection or from preemptive culling (22). The implementation of vaccination of poultry as a tool for the reduction of the viral load in the environment and, thus, for decreasing the risk of transmission within poultry-and, as a consequence, to humans-is still a discussed topic. Inactivated vaccines are the most widely used vaccines in AI vaccination programs. They are particularly addressed to protect adult chickens, turkeys, and other birds in emergency situations, e.g., when ring vaccination is used in an area of an HPAI virus (HPAIV) outbreak or when prophylactic vaccination is used in a region where H5 or H7 AI virus (AIV) infections are endemic. However, these vaccines limited the serological detection of wild-type AIV infections in immunized populations, as wild-type infection could be detectable only through higher antibody titers to nonstructural proteins or if the neuraminidase subtype of the vaccine differed from the subtype of the introduced wild-type virus (28).The use of vaccines that fit in any case to the strategy of differentiating infected from vaccinated animals (DIVA) would make a strong case for turning away from current stamping-out policies in many countries. Vaccines that consist of only one major antigenic protein related to influenza A virus (e.g., hemagglutini...

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“…However, vaccines were administered by injection and also required purification steps (Farrance et al, 2011). It has been suggested that the use of plant- based oral vaccines for booster immunization, with injected vaccination for primary immunization, may be more effective than current strategies (Wu et al, 2004;Thanavala et al, 2005). Here we present data on the evaluation of efficacy, especially infection-preventing effectiveness, of a plant-based oral vaccine against protozoan infectious disease.…”

Section: Discussionmentioning

confidence: 99%

Booster responses by oral vaccination with transgenic plants against chicken leucocytozoonosis

et al. 2013

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We developed a transgenic potato (TrP/R7) expressing the recombinant R7 (rR7) antigen for use as an oral vaccine to protect against a chicken protozoan disease, chicken leucocytozoonosis. The TrP/R7 potato was produced by Agrobacterium tumefaciens-mediated transformation and regeneration, and the R7 gene insertion into potato chromosomes was confirmed by genomic polymerase chain reaction and Southern hybridization. rR7 antigen expression in TrP/R7 potato was also confirmed by sandwich enzyme-linked immunosorbent assay and western blotting using an antibody against the second-generation schizont of Leucocytozoon caulleryi. A transgenic potato clone with the highest rR7 antigen expression (3 mg rR7 antigen per gram of fresh-weight potato leaves) was selected, cultivated, and used in oral administration experiments to examine its ability to boost immunity. Chickens were immunized with chicken leucocytozoonosis vaccine ''Hokken'' by injection, and chickens that developed moderate levels of antibody titres were fed with TrP/R7 leaves. Chickens fed with TrP/R7 leaves showed increased antibody responses. In contrast, chickens fed with non-transgenic potato leaves showed a continuous decrease in antibody titres. Furthermore, chickens fed with TrP/R7 potato leaves showed strong resistance against experimental challenge with L. caulleryi infection. This study demonstrates the use of a plant-based oral vaccine to boost immunity against a protozoan disease.

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Immunization of Chickens with VP2 Protein of Infectious Bursal Disease Virus Expressed in Arabidopsis thaliana

Cited by 41 publications

References 22 publications

Current status of vaccines against infectious bursal disease

Current status of vaccines against infectious bursal disease

Immunization with Plant-Expressed Hemagglutinin Protects Chickens from Lethal Highly Pathogenic Avian Influenza Virus H5N1 Challenge Infection

Booster responses by oral vaccination with transgenic plants against chicken leucocytozoonosis

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