Immunization against experimental murine salmonellosis with liposome-associated O-antigen

Desiderio, J. V.; Campbell, S G

doi:10.1128/iai.48.3.658-663.1985

Cited by 35 publications

(6 citation statements)

References 34 publications

(24 reference statements)

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“…Delayed type hypersensitivity (DTH) was checked using the method of Desiderio & Campbell (1985). The mice were divided into different groups: control (untreated saline); immunized (days 0 and 14) with 50 ìg IROMP, with 2 .…”

Section: Methodsmentioning

confidence: 99%

Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen

Chibber

Bhardwaj

2004

Journal of Medical Microbiology

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Vi polysaccharide and iron-regulated outer-membrane proteins (IROMPs) were extracted and purified from the standard strain of Salmonella typhi, Ty2. These were then conjugated by chemical coupling using the carbodimide method. Vi-IROMP conjugate was tested for its ability to protect against colonization by S. typhi in different organs. Mice immunized with 2 . 5 ìg Vi-IROMP conjugate showed the most protection, as the least bacterial colonization of spleen, liver and Peyer's patches was observed. Peritoneal macrophages obtained from conjugate-treated mice phagocytosed bacteria efficiently. Circulating antibodies and the delayed type hypersensitivity response elucidated by mouse foot-pad swelling was significantly higher in conjugate-treated animals. These results clearly demonstrate that an IROMP and polysaccharide conjugate of S. typhi prepared from the same strain has the potential to protect animals against challenge.

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Section: Methodsmentioning

confidence: 99%

Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen

Chibber

Bhardwaj

2004

Journal of Medical Microbiology

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“…Attempts aimed at enhancing the immunogenic properties of LPS and lipid A were also made earlier (4,8). Thus, Salmonella typhimurium LPS, incorporated into liposomes, effectively induced delayed-type hypersensitivity.…”

Section: Prepna Dosementioning

confidence: 99%

Toxicity and immunogenicity of Neisseria meningitidis lipopolysaccharide incorporated into liposomes

et al. 1992

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To obtain nontoxic and highly immunogenic lipopolysaccharide (LPS) for immunization, we incorporated Neisseria meningitidis LPS into Uposomes. Native LPS and its salts were incorporated by the method of dehydration-rehydration of vesicles or prolonged cosonication. The most complete incorporation of LPS into Uposomes and a decrease in toxicity were achieved by the method of dehydration-rehydration of vesicles. Three forms of LPS (H+ form, Mg2e salt, and triethanolamine salt) showed different solubilties in water, the acidic form of LPS, with the most pronounced hydrophobic properties, being capable of practically complete association with liposomal membranes. An evaluation of the activity of liposomal LPS in vitro (by the Limulus amoebocyte test) and in vivo (by monitoring the pyrogenic reaction in rabbits) revealed a decrease in endotoxin activity of up to 1,000-fold. In addition, the pyrogenic activity of liposomal LPS was comparable to that of a meningococcal polysaccharide vaccine. Liposomes had a pronounced adjuvant effect on the immune response to LPS. Thus, the level of anti-LPS plaque-forming ceHls in the spleens of mice immunized with liposomal LPS was 1 order of magnitude higher and could be observed for a longer time (until day 21, i.e., the term of observation) than in mice immunized with free LPS. The same regularity was revealed in a study done with an enzyme-linked immunosorbent assay. This study also established that antibodies induced by immunization belonged to the immunoglobulin M and G classes, which are capable of prolonged circulation. Moreover, liposomal LPS induced a pronounced immune response in CBA/N mice (defective in B lymphocytes of the LyB-5+ subpopulation). The latter results indicate that the immunogenic action of liposomal LPS occurs at an

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“…5 The treatment involves seven days of antibiotic therapy administered orally or by intravenous route. Existing carrier systems include liposomes, [6][7][8][9][10][11][12] microparticles 13,14 and nanoparticles. 15 These systems suffer from the limitation that they are not robust enough to be delivered orally.…”

Section: Introductionmentioning

confidence: 99%

Lipid coated mesoporous silica nanoparticles as an oral delivery system for targeting and treatment of intravacuolar Salmonella infections

2014

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Lipid coated mesoporous silica nanoparticle (L-MSN) were synthesized for oral delivery of ciprofloxacin for intracellular elimination of Salmonella pathogen. The particle size was found to be between 50-100 nm with a lipid coat of approximately 5 nm thickness. The lipid coating was achieved by sonication of liposomes with the MSN particles and evaluated by CLSM and FTIR studies. The L-MSN particles exhibited lower cytotoxicity compared to bare MSN particles. Ciprofloxacin, a fluoroquinolone antibiotic, loaded into the L-MSN particles showed enhanced antibacterial activity against free drug in in vitro assays. The lipid coat was found to aid in intravacuolar targeting of the drug cargo as observed by confocal microscopy studies. We also observed that a lower dose of antibiotic was sufficient to clear the pathogen from mice and increase their survivability using the L-MSN oral delivery system.

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Immunization against experimental murine salmonellosis with liposome-associated O-antigen

Cited by 35 publications

References 34 publications

Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen

Protection in a mouse peritonitis model mediated by iron-regulated outer-membrane protein of Salmonella typhi coupled to its Vi antigen

Toxicity and immunogenicity of Neisseria meningitidis lipopolysaccharide incorporated into liposomes

Lipid coated mesoporous silica nanoparticles as an oral delivery system for targeting and treatment of intravacuolar Salmonella infections

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