Immunization against dental caries

Bowen, Willis H.; Cohen, B. J.; Cole, Michael F.; Colman, G.

doi:10.1038/sj.bdj.4803490

Cited by 87 publications

(36 citation statements)

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“…The variability of previous reports (Hayashi et al, 1972;Tanzer et al, 1973 ;Taubman and Smith, 1974;Bowen et al, 1975) may be due to fluctuation in the number of S. mutans in plaque samples, and the quantitative errors inherent in single estimations and the technique of bacteriological counting. In a longitudinal study, such fluctuations can be detected and a continuous assessment of differences between immunised and control animals can be made.…”

Section: Discussionmentioning

confidence: 89%

A Sequential Bacteriological and Serological Investigation of Rhesus Monkeys Immunised Against Dental Caries with Streptococcus Mutans

Caldwell

Challacombe

Lehner

1977

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 89%

A Sequential Bacteriological and Serological Investigation of Rhesus Monkeys Immunised Against Dental Caries with Streptococcus Mutans

Caldwell

Challacombe

Lehner

1977

Journal of Medical Microbiology

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“…An earlier report from this laboratory described successful protection of monkeys immunized with walls of S. mutans (Bowen et al, 1975). Walls treated with trypsin, on the other hand, fail to confer protection suggesting that the protective antigen might be protein in nature (Colman & Cohen, 1979).…”

Section: Introductionmentioning

confidence: 99%

“…Of particular concern in the case of S. mutans is the demonstration of an antigenic relationship between S. mutans and human heart tissue (van de Rijn et al, 1976). An earlier report from this laboratory described successful protection of monkeys immunized with walls of S. mutans (Bowen et al, 1975). Walls treated with trypsin, on the other hand, fail to confer protection suggesting that the protective antigen might be protein in nature (Colman & Cohen, 1979).…”

mentioning

confidence: 99%

Wall-associated Protein Antigens of Streptococcus mutans

Russell

1979

Journal of General Microbiology

231

203

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109When heat-killed whole organisms of Streptococcus mutans strain Ingbritt (serotype c ) were injected into rabbits, antibodies to at least 12 antigens were detectable by crossed immunoelectrophoresis. In contrast, when rabbits were immunized with organisms which had been subjected to extraction with the detergent sodium dodecyl sulphate (SDS), antibodies to only two protein antigens were found. These two proteins (A and B), while existing in a form apparently closely associated with peptidoglycan, could also be recovered from homogenates of whole organisms after sonication and from culture filtrates. Antigenic material was excreted throughout growth. SDS-polyacrylamide gradient gel electrophoresis showed A to have a molecular weight of 29000, while B had a molecular weight of 190000. Antigen B was purified to apparent homogeneity as judged by SDS-polyacrylamide gel electrophoresis and isoelectric focusing. All of six strains of serotype c examined produced antigen B. Strains of serotypes e and f also produce antigenically identical proteins and strains of serotypes d and g produce proteins which cross-reacted with antigen B. Antigen B was specifically precipitated by rabbit antiserum to human heart tissue. I N T R O D U C T I O NExperiments from a number of laboratories have clearly shown that immunization of animals with Streptococcus mutans or its subcellular fractions can confer protection against dental caries induced by this organism (Bowen et al., 1976), but the mechanism of this protection and the bacterial antigens involved remain unknown. For the development of an effective vaccine it is desirable to identify the antigen(s) involved in eliciting protective antibody, and the means by which protection is conferred. Identification and purification of such an antigen would aid in the understanding of the mechanism of protection, allow monitoring of vaccine preparations for efficacy, and facilitate study of its relationship with any toxic components of a crude vaccine. Of particular concern in the case of S. mutans is the demonstration of an antigenic relationship between S. mutans and human heart tissue (van de Rijn et al., 1976).An earlier report from this laboratory described successful protection of monkeys immunized with walls of S. mutans (Bowen et al., 1975). Walls treated with trypsin, on the other hand, fail to confer protection suggesting that the protective antigen might be protein in nature (Colman & Cohen, 1979). The present paper describes studies of proteins associated with S. mutans walls and the purification of one wall-associated antigen. The purified antigen is also demonstrated to be antigenically related to human heart tissue. M E T H O D R. R. B. R U S S E L Lyeast extract, 3 g; NaCI, 2 g; K2HP04, 2 g; NaHCO,, 1 g; glucose, 5 g; water, 1 1) and the chemically defined (CD) medium of Janda & Kuramitsu (1976) supplemented with 0.2% (w/v) Na,CO, (Terleckyj & Shockman, 1975). Antisera. New Zealand white rabbits (each 2 to 3 kg) were used for the preparation of antisera. Injection schedule...

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“…The results with crude GTase preparations as antigens in animals have been variable (2,11,19,37), and no conclusion can be drawn at this point. In this connection, it was recently demonstrated (1) that antisera from monkeys immunized with GTase of S. mutans strain FA1 reduced the synthesis of glucan from sucrose.…”

mentioning

confidence: 99%