Immunity to the protozoan parasite Eimeria nieschulzi in inbred CD-F rats

Liburd, E.M.; Armstrong, W. D.; Mahrt, Jerome L.

doi:10.1016/0008-8749(73)90208-6

Cited by 25 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The course of infection with E. vermiformis was compared in four groups of mice, namely, thymectomized L3T4+ (8) 58.8 ± 4.2t 9.4 t 0.5t 0.47 ± 0.09t 0.73 ± 0.09t None (11) 52.9 ± 3.1t 9.0 ± 0.4t 0.53 ± 0.05t 0.74 ± 0.08t aMice were thymectomized at 4.5 weeks of age, followed by two injections, each of 400 iLg, of an antibody in 0.2 ml (or 0.2 ml of 10% fetal calf serum for controls) given i.v. 10 and 16 days after thymectomy.…”

Section: Resultsmentioning

confidence: 99%

“…This host-parasite relationship is similar in many respects to those in the systems of agricultural importance, and the mouse as host offers many advantages for immunologic investigations. Earlier attempts to transfer immunity adoptively have been successful in the rat (11,18,20) and in domestic fowl (23), but they necessitated the injection of very large numbers of cells. This requirement appeared to apply also in the initial work with mice, when mixtures of spleen and mesenteric lymph node (MLN) cells were used, but subsequently, it was found that protection was readily transferred with 5 x 107 MLN cells (32).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mediation of immunity to Eimeria vermiformis in mice by L3T4+ T cells

et al. 1988

View full text Add to dashboard Cite

Immunity to infection with Eimeria vermiformis was transferred in NIH mice by both the nylon wooladherent (B-cell-enriched) and nonadherent (T-cell-enriched) fractions of lymphocytes (spleen and mesenteric lymph node) taken from infected donors. Transfer was more variable with the adherent fraction, and when contaminating T cells were removed by treatment with anti-Thyl monoclonal antibody (MAb) and complement, this fraction lost all protective activity. The protective effect of T-cell-enriched populations of mesenteric lymphocytes was abrogated by treatment with anti-L3T4 MAb and complement in vitro before transfer or by opsonization with this MAb in vitro before intravenous inoculation into recipients. Similar treatments of cells with anti-Lyt2 MAb did not have this effect, confirming that Thyl+ L3T4+ cells mediate the adoptive transfer of immunity to E. vermiformis. Thyl+ L3T4+ cells were also shown to limit the replication of E. vermiformis in primary infections: mice depleted of this subset (by thymectomy followed by intravenous injection of anti-L3T4 MAb) passed greater numbers of oocysts over a longer period of time than did mice similarly depleted of Lyt2+ cells.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Mediation of immunity to Eimeria vermiformis in mice by L3T4+ T cells

et al. 1988

View full text Add to dashboard Cite

show abstract

“…As a preliminary to the exploitation of this feature for the analysis of protective immune mechanisms it was necessary to establish a reliable procedure for the adoptive transfer of immunity. In coccidiosis adoptive transfer has previously been described in rats infected with E. nieschulzi (Wittchow 1972, Rommel & Heydorn 1971, Liburd, Armstrong & Mahrt 1973, Ogilvie & Rose 1977 and in chickens infected with E. maxima (Rose & Hesketh 1982). Common to all these reports has been the necessity to transfer very large numbers of cells in order to confer a substantial degree of protection.…”

Section: Introductionmentioning

confidence: 99%

Immunity to coccidiosis: adoptive transfer in NIH mice challenged with Eimeria vermiformis

Rose

Wakelin

Joysey

et al. 1988

Parasite Immunology

View full text Add to dashboard Cite

The development of a reliable model for the adoptive transfer of immunity to coccidiosis (infection with Eimeria vermiformis in NIH mice) is described. More than 10(8) of a mixture of spleen and mesenteric lymph node (MLN) cells, given either intravenously or intraperitoneally, were required to transfer a significant degree of protection. Dividing cells, present in the donors at 10 or 14 days after priming, but not at 5 or 19 days, were shown to be the effectors. When examined separately, MLN cells were found to be superior to spleen cells, and the injection of as few as 5 x 10(7) was capable of substantially reducing the oocyst output from a challenge inoculum. The recipients of cells from primed mice had earlier, and sometimes higher, titres of specific antibodies in the serum but, overall, there was no correlation between these titres and protection. Further characterization of the cells responsible for adoptively transferring immunity to this infection should now be possible.

show abstract

“…Although cell-mediated immunity (CMI) has been shown to play a major role in resistance to coccidia in other animal species (Klesius et al, 1975;Klesius et al, 1976;Liburd et al, 1973), little published work of this nature is available for chickens. Morita et al (1973) reported that a CMI response for E. tenella infections in chickens was detected by splenic cell migration inhibition.…”

Section: Introductionmentioning

confidence: 99%

Avian Coccidiosis: Evidence for a Cell-Mediated Immune Response

1980

View full text Add to dashboard Cite

A cell-mediated immune (CMI) response to chicken coccidia oocyst antigen extracts was shown in Single Comb White Leghorn chickens vaccinated with a commercially available coccidiosis vaccine (CocciVac® D) by delayed hypersensitivity (DH) and leukocyte stimulation. A particulate oocyst antigen from Eimeria necatrix gave positive DH wattle reaction in 10 of 20, 9-week-old chickens vaccinated at 4 weeks of age and 10 of 20, 33-week-old chickens which received 3 additional vaccinations at monthly intervals starting at 18 weeks of age. In contrast, only 4 of 20, 31-week-old chickens which received the 3 additional vaccinations gave positive stimulation reactions with blood derived leukocytes against alkali soluble oocyst antigen extracts from particulate oocyst antigens of E. necatrix and E. maxima. Forty unvaccinated control chickens did not produce significant DH reactions, although 1 of 20, 31-week-old control chickens did produce a positive stimulation response. The ability of the chicken to mount a measurable CMI response correlated with disease resistance. All chickens which produced a significant CMI response were totally refractory to challenge with either E. necatrix at 9 weeks or E. tenella at 3 3 weeks, while all but 1 unvaccinated chicken, which failed to produce positive cell-mediated immunity (CMI) reactions were susceptible to challenge. In addition, 2 of 20 and 2 of 10 vaccinated chickens which did not produce a significant CMI response at 9 and 33 weeks of age, respectively, were susceptible to challenge.

show abstract

Immunity to the protozoan parasite Eimeria nieschulzi in inbred CD-F rats

Cited by 25 publications

References 14 publications

Mediation of immunity to Eimeria vermiformis in mice by L3T4+ T cells

Mediation of immunity to Eimeria vermiformis in mice by L3T4+ T cells

Immunity to coccidiosis: adoptive transfer in NIH mice challenged with Eimeria vermiformis

Avian Coccidiosis: Evidence for a Cell-Mediated Immune Response

Contact Info

Product

Resources

About