Immunhistologischer Nachweis von Typ I- und Typ III-Kollagen in Leberbiopsien: Frühe und späte Veränderungen bei alkoholischer Lebererkrankung

Ott, Ursula; Hahn, Eckhart G.; Moshudis, E; Jc, Bode; Martini, G. A.

doi:10.1007/978-3-642-85452-1_149

Cited by 4 publications

(2 citation statements)

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“…Whether fibronectin is associated with the perisinusoidal basement membrane or with underlying collagen fibrils (Schaffner and Popper, 1963) remains to be determined by better resolving techniques such as immunoelectronmicroscopy. Collagens and procollagens type I and III have been demonstrated in the perisinusoidal space by immunofluorescence studies (Gay et al, 1975;Ott et al, 1977;Wick et al, 1978) and codistribution of these collagens with fibronectin was found in cell culture studies .…”

Section: Discussionmentioning

confidence: 98%

“…These changes may cause severe restrictions in the normal function of liver cells. Some progress has been made recently in analysing human liver fibrosis at the level of interstitial collagens using biochemical (Rojkind and Martinez-Palomo, 1976;Seyer et al, 1977;Rojkind et al, 1979) and immunohistological methods (Ott et al, 1977;Wick et al, 1978). The data showed increased deposition of collagens type I and III in both periportal and intralobular areas.…”

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confidence: 99%

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Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin.

Hahn¹,

Wick²,

Pencev³

et al. 1980

Gut

380

163

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SUMMARY Specific antibodies to collagen type IV, laminin, and fibronectin were used to localise these proteins by indirect immunofluorescence in frozen sections of normal and fibrotic liver. In normal livers distinct staining was found in basement membranes of blood and lymph vessels, of bile ducts and ductules and around nerve axons. Positive reactions for type IV collagen and fibronectin were also observed in the perisinusoidal space, while hepatocytes and most of the interstitial matrix of portal fields remained unstained. Liver specimens obtained from patients with alcoholic liver disease (fatty liver, hepatitis or cirrhosis) and chronic active hepatitis showed a more intense reaction with the antibodies in the perisinusoidal space including now distinct staining for laminin. These patterns were particularly prominent at borders between fibrotic septa and remnants of parenchyma or pseudolobules. Strong reactions were also found for type IV collagen and fibronectin in the periportal interstitium and in large fibrotic areas. The findings support previous electronmicroscopical and chemical evidence for increased basement membrane production in human liver fibrosis and demonstrate that this may involve different proteins and occur at different anatomical sites.

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin.

Hahn¹,

Wick²,

Pencev³

et al. 1980

Gut

380

163

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Alcoholic liver disease: Information in search of knowledge?

1981

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Connective tissue components of the normal and fibrotic liver

et al. 1981

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The first part of this review describes the chemistry, the occurrence and the metabolism of extracellular connective tissue components in the liver. The normal liver contains typical connective tissue proteins (collagens, structural glycoproteins and proteoglycans) not only in vessel walls, perivascular areas and in the capsule, but they occur also in small amounts in the parenchyma, mainly in the space of Disse along the sinusoidal walls. The "interstitial" collagens type I and III represent the major amount of collagen in the normal as well as in fibrotic liver, showing a relative increase of type III in fibrosis. Basement membrane collagens type IV and V as well as the cysteine-rich collagenous components "7 S collagen" and "short chain collagen" have been shown to occur in extracts prepared after limited pepsin digestion. In the normal liver, basement membrane collagen can hardly be detected within the parenchyma by immunofluorescence microscopy; increased occurrence, however, can be shown along the sinusoids even in early stages of chronic liver diseases. The glycoprotein fibronectin was shown to be distributed very similarly to collagens type I and III, whereas the basement membrane specific glycoprotein laminin is restricted to vessel walls and the epithelial layer of bile ductuli in the normal liver but is also found in the parenchyma in fibrosis. Occurrence of proteoglycans is increased in fibrosis: a change in the composition of glycosaminoglycans from mainly heparan sulfate in the normal to dermatan- and chondroitin sulfate in the fibrotic liver was observed. It is not yet clear which cell type is mainly responsible for increased connective tissue synthesis in fibrosis. The occurrence of cells resembling smooth muscle cells ("myofibroblasts") in connective tissue septa of fibrotic livers and the fact that similar cells which actively synthesize collagen grow from explants of fibrotic livers may indicate the significance of this cell type in the process of liver fibrosis.

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Immunhistologischer Nachweis von Typ I- und Typ III-Kollagen in Leberbiopsien: Frühe und späte Veränderungen bei alkoholischer Lebererkrankung

Cited by 4 publications

References 6 publications

Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin.

Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin.

Alcoholic liver disease: Information in search of knowledge?

Connective tissue components of the normal and fibrotic liver

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